中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
4期
482-484
,共3页
范莹%张晓杉%罗松辉%黄超
範瑩%張曉杉%囉鬆輝%黃超
범형%장효삼%라송휘%황초
糖尿病,2型%维生素D%心肌肥厚
糖尿病,2型%維生素D%心肌肥厚
당뇨병,2형%유생소D%심기비후
Diabetes,type 2%Vitamin D%Cardiac hypertrophy
目的 建立糖尿病大鼠心肌肥厚模型,观察维生素D对2型糖尿病大鼠心肌肥厚干预作用并分析其机制.方法 将采用链脲佐菌素(STZ)腹腔注射建立糖尿病模型成功后并经超声检查确认存在心肌肥厚的19只雄性Wistar大鼠随机分为糖尿病心肌肥厚组(7只)、胰岛素干预组(6只)、维生素D干预组(6只),6只健康Wistar大鼠作为正常对照组.胰岛素组皮下注射常规胰岛素25 U/kg 1次/d,持续28 d；维生素D组给予1,25-(OH)2D32 μg/(kg·d)灌胃,持续28 d.每周检测各组大鼠空腹血糖及胰岛素水平；全部动物于4周后处死,测定左心室质量指数、心肌胶原含量、心肌胶原容积分数,并用蛋白质印迹法检测维生素D受体表达情况.结果 与正常对照组比较,糖尿病心肌肥厚组大鼠胰岛素水平明显降低[(3.8±1.3) mU/L比(24.4 ±2.5)mU/L] (P ＜0.05),空腹血糖,左心室质量指数、心肌胶原含量、心肌胶原容积分数及维生素D受体均明显增加[(28.7±0.5)mmol/L比(4.8±1.1) mmol/L,(2.40±0.46) mg/g比(1.92±0.25) mg/g,(1.93±0.42)mg/g比(1.02±0.28)mg/g,(6.51±1.28)％比(2.64±0.63)％,0.51(VDR/GAPDH比值)比0.24] (P ＜0.05)；与糖尿病心肌肥厚组比较,胰岛素干预组或维生素D干预组胰岛素浓度明显升高,余各项指标明显降低(P＜0.05).结论 维生素D对2型糖尿病大鼠心肌肥厚有一定逆转作用,其机制可能与调节血糖水平、刺激胰岛素分泌间接作用,和/或通过其受体对肥厚心肌直接作用有关.
目的 建立糖尿病大鼠心肌肥厚模型,觀察維生素D對2型糖尿病大鼠心肌肥厚榦預作用併分析其機製.方法 將採用鏈脲佐菌素(STZ)腹腔註射建立糖尿病模型成功後併經超聲檢查確認存在心肌肥厚的19隻雄性Wistar大鼠隨機分為糖尿病心肌肥厚組(7隻)、胰島素榦預組(6隻)、維生素D榦預組(6隻),6隻健康Wistar大鼠作為正常對照組.胰島素組皮下註射常規胰島素25 U/kg 1次/d,持續28 d；維生素D組給予1,25-(OH)2D32 μg/(kg·d)灌胃,持續28 d.每週檢測各組大鼠空腹血糖及胰島素水平；全部動物于4週後處死,測定左心室質量指數、心肌膠原含量、心肌膠原容積分數,併用蛋白質印跡法檢測維生素D受體錶達情況.結果 與正常對照組比較,糖尿病心肌肥厚組大鼠胰島素水平明顯降低[(3.8±1.3) mU/L比(24.4 ±2.5)mU/L] (P ＜0.05),空腹血糖,左心室質量指數、心肌膠原含量、心肌膠原容積分數及維生素D受體均明顯增加[(28.7±0.5)mmol/L比(4.8±1.1) mmol/L,(2.40±0.46) mg/g比(1.92±0.25) mg/g,(1.93±0.42)mg/g比(1.02±0.28)mg/g,(6.51±1.28)％比(2.64±0.63)％,0.51(VDR/GAPDH比值)比0.24] (P ＜0.05)；與糖尿病心肌肥厚組比較,胰島素榦預組或維生素D榦預組胰島素濃度明顯升高,餘各項指標明顯降低(P＜0.05).結論 維生素D對2型糖尿病大鼠心肌肥厚有一定逆轉作用,其機製可能與調節血糖水平、刺激胰島素分泌間接作用,和/或通過其受體對肥厚心肌直接作用有關.
목적 건립당뇨병대서심기비후모형,관찰유생소D대2형당뇨병대서심기비후간예작용병분석기궤제.방법 장채용련뇨좌균소(STZ)복강주사건립당뇨병모형성공후병경초성검사학인존재심기비후적19지웅성Wistar대서수궤분위당뇨병심기비후조(7지)、이도소간예조(6지)、유생소D간예조(6지),6지건강Wistar대서작위정상대조조.이도소조피하주사상규이도소25 U/kg 1차/d,지속28 d；유생소D조급여1,25-(OH)2D32 μg/(kg·d)관위,지속28 d.매주검측각조대서공복혈당급이도소수평；전부동물우4주후처사,측정좌심실질량지수、심기효원함량、심기효원용적분수,병용단백질인적법검측유생소D수체표체정황.결과 여정상대조조비교,당뇨병심기비후조대서이도소수평명현강저[(3.8±1.3) mU/L비(24.4 ±2.5)mU/L] (P ＜0.05),공복혈당,좌심실질량지수、심기효원함량、심기효원용적분수급유생소D수체균명현증가[(28.7±0.5)mmol/L비(4.8±1.1) mmol/L,(2.40±0.46) mg/g비(1.92±0.25) mg/g,(1.93±0.42)mg/g비(1.02±0.28)mg/g,(6.51±1.28)％비(2.64±0.63)％,0.51(VDR/GAPDH비치)비0.24] (P ＜0.05)；여당뇨병심기비후조비교,이도소간예조혹유생소D간예조이도소농도명현승고,여각항지표명현강저(P＜0.05).결론 유생소D대2형당뇨병대서심기비후유일정역전작용,기궤제가능여조절혈당수평、자격이도소분비간접작용,화/혹통과기수체대비후심기직접작용유관.
Objective To investigate left ventricular hypertrophy in type 2 diabetes rats and to explore the effects of vitamin D on the rats.Methods Forty Wistar rats were injected intraperitoneally with STZ and 37 rats were induced as diabetes successfully.After 8 weeks fostered,19 rats were identified as diabetes with left ventricular hypertrophy by ultrasound examination.They were randomly treated with insulin and vitamin D as experimental group.Six healthy Wistar rats were used as control.Fasting blood glucose and insulin were measured weekly.Left ventricular mass index,myocardial collagen content,myocardial collagen volume fraction and vitamin D receptors were detected at 4 weeks later.Results Compared to the normal control group,insulin level decreased significantly (P ＜ 0.05) but the fasting blood glucose level,left ventricular mass index,myocardial collagen content,myocardial collagen volume fraction and expression of vitamin D receptors were increased significantly in experimental group (P ＜ 0.05).Compared to the model group,insulin level in insulin treated group and vitamin D treated group increased significantly (P ＜ 0.05),and the other index decreased significantly (P ＜ 0.05).Conclusion Vitamin D can reverse cardiac hypertrophy in diabetes rats and the mechanism may involve reducing blood glucose and stimulating insulin secretion,and (or) reversing myocardial hypertrophy via vitamin D receptors directly.
