中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
5期
676-677
,共2页
高效液相色谱%七叶灵颗粒%柚皮苷
高效液相色譜%七葉靈顆粒%柚皮苷
고효액상색보%칠협령과립%유피감
High performance liquid chromatography%Qiyelin granules%Naringin
目的 建立用高效液相色谱法测定七叶灵颗粒中柚皮苷含量的方法.方法 使用KromasilC18色谱柱(250.0mm×4.6 mm,5μm),流动相为乙腈∶4%冰醋酸(17∶ 83),检测波长为224 nm,流速:1 ml/min,柱温30℃.结果 该色谱条件下显示能良好分离柚皮苷峰,避免杂质干扰,柚皮苷在0.08~2.00 μg范围线性关系良好(r=0.9999),精密度相对标准偏差(RSD)=1.97%(n=5).柚皮苷平均加样回收率为102.60%,RSD=1.86% (n =6).结论 用高效液相色谱法测定七叶灵颗粒中柚皮苷含量方法简便,重现性好,经过多批制剂检测,结果稳定,可用于七叶灵颗粒中柚皮苷的含量测定.
目的 建立用高效液相色譜法測定七葉靈顆粒中柚皮苷含量的方法.方法 使用KromasilC18色譜柱(250.0mm×4.6 mm,5μm),流動相為乙腈∶4%冰醋痠(17∶ 83),檢測波長為224 nm,流速:1 ml/min,柱溫30℃.結果 該色譜條件下顯示能良好分離柚皮苷峰,避免雜質榦擾,柚皮苷在0.08~2.00 μg範圍線性關繫良好(r=0.9999),精密度相對標準偏差(RSD)=1.97%(n=5).柚皮苷平均加樣迴收率為102.60%,RSD=1.86% (n =6).結論 用高效液相色譜法測定七葉靈顆粒中柚皮苷含量方法簡便,重現性好,經過多批製劑檢測,結果穩定,可用于七葉靈顆粒中柚皮苷的含量測定.
목적 건립용고효액상색보법측정칠협령과립중유피감함량적방법.방법 사용KromasilC18색보주(250.0mm×4.6 mm,5μm),류동상위을정∶4%빙작산(17∶ 83),검측파장위224 nm,류속:1 ml/min,주온30℃.결과 해색보조건하현시능량호분리유피감봉,피면잡질간우,유피감재0.08~2.00 μg범위선성관계량호(r=0.9999),정밀도상대표준편차(RSD)=1.97%(n=5).유피감평균가양회수솔위102.60%,RSD=1.86% (n =6).결론 용고효액상색보법측정칠협령과립중유피감함량방법간편,중현성호,경과다비제제검측,결과은정,가용우칠협령과립중유피감적함량측정.
Objective To establish a high performance liquid chromatography method for determination naringin in Qiyelin granules.Methods Kromasil C18 column(250.0 mm × 4.6 mm,5 μm) was used; the mobile phase was acetonitrile∶ 4% acetic acid (17∶ 83).The detection wavelength was 224 nm; the flow rate was 1 ml/min and the column temperature was 30 °C.Results This method determinated the naringin effectively and avoided the impurity.The linear range of naringin was at 0.08-2 μg (r =0.9999),relative standard deviation (RSD) was 1.97 % (n =5).The average recovery of naringin was 102.6%,RSD =1.86% (n =6).Conclusions The method of Qiyeling particles in naringin determined by high performance liquid chromatography is simple and repeatable.The result is stable.It can be used for determination the content of naringin in Qiyelin granules.