中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
11期
1605-1607
,共3页
章岳峰%张静%林加锋%丁云开%叶松道
章嶽峰%張靜%林加鋒%丁雲開%葉鬆道
장악봉%장정%림가봉%정운개%협송도
海洛因依赖者%辅助性T细胞1%辅助性T细胞2
海洛因依賴者%輔助性T細胞1%輔助性T細胞2
해락인의뢰자%보조성T세포1%보조성T세포2
Heroin addicts%Helper T cell 1%Helper T cell 2
目的 研究海洛因依赖者血清中辅助性T细胞1(Th1)/Th2类细胞因子的表达水平,进而对海洛因依赖者的免疫状况进行评价.方法 选择2012年7月至2013年6月于温州市三垟强制隔离戒毒所收容的50例海洛因依赖者作为海洛因依赖组,选择同期于我院行健康体检的50例正常人作为正常对照组.采取放射免疫法检测2组受试对象血清中白细胞介素2(IL-2)、y干扰素(IFN-y)、肿瘤坏死因子α(TNF-α)、TNF-β、IL-4、IL-6、IL-8、IL-10和IL-17的表达水平.结果 海洛因依赖组血清中Th1细胞因子IL-2、IFN-γ、TNF-α和TNF-β水平均明显低于正常对照组[分别为(118±38) ng/L比(249±76) ng/L、(263±78) ng/L比(492±154) ng/L、(103±22) ng/L比(121±23) ng/L、(72±26) ng/L比(118±34) ng/L],差异均有统计学意义(t=3.154,t=4.518,t=2.459,t=3.089,均P＜0.05)；Th2细胞因子IL-4、IL-6、IL-8、IL-10和IL-17的表达水平均明显高于正常对照组[分别为(252±72) ng/L比(124±32) ng/L、(4.4±1.6) ng/L比(2.8±1.0) ng/L、(409±112)ng/L比(214±67) ng/L、(12.1±3.4)ng/L比(8.2±2.0)ng/L、(164±50) ng/L比(95±28)ng/L],差异均有统计学意义(t=3.157,t =2.589,t=3.872,t=2.711,=3.255,均P＜0.05).结论 海洛因依赖者血清中Th 1/Th2类细胞因子水平较正常对照严重失衡,提示其免疫功能较差,易获得外界感染,需给予足够重视.
目的 研究海洛因依賴者血清中輔助性T細胞1(Th1)/Th2類細胞因子的錶達水平,進而對海洛因依賴者的免疫狀況進行評價.方法 選擇2012年7月至2013年6月于溫州市三垟彊製隔離戒毒所收容的50例海洛因依賴者作為海洛因依賴組,選擇同期于我院行健康體檢的50例正常人作為正常對照組.採取放射免疫法檢測2組受試對象血清中白細胞介素2(IL-2)、y榦擾素(IFN-y)、腫瘤壞死因子α(TNF-α)、TNF-β、IL-4、IL-6、IL-8、IL-10和IL-17的錶達水平.結果 海洛因依賴組血清中Th1細胞因子IL-2、IFN-γ、TNF-α和TNF-β水平均明顯低于正常對照組[分彆為(118±38) ng/L比(249±76) ng/L、(263±78) ng/L比(492±154) ng/L、(103±22) ng/L比(121±23) ng/L、(72±26) ng/L比(118±34) ng/L],差異均有統計學意義(t=3.154,t=4.518,t=2.459,t=3.089,均P＜0.05)；Th2細胞因子IL-4、IL-6、IL-8、IL-10和IL-17的錶達水平均明顯高于正常對照組[分彆為(252±72) ng/L比(124±32) ng/L、(4.4±1.6) ng/L比(2.8±1.0) ng/L、(409±112)ng/L比(214±67) ng/L、(12.1±3.4)ng/L比(8.2±2.0)ng/L、(164±50) ng/L比(95±28)ng/L],差異均有統計學意義(t=3.157,t =2.589,t=3.872,t=2.711,=3.255,均P＜0.05).結論 海洛因依賴者血清中Th 1/Th2類細胞因子水平較正常對照嚴重失衡,提示其免疫功能較差,易穫得外界感染,需給予足夠重視.
목적 연구해락인의뢰자혈청중보조성T세포1(Th1)/Th2류세포인자적표체수평,진이대해락인의뢰자적면역상황진행평개.방법 선택2012년7월지2013년6월우온주시삼양강제격리계독소수용적50례해락인의뢰자작위해락인의뢰조,선택동기우아원행건강체검적50례정상인작위정상대조조.채취방사면역법검측2조수시대상혈청중백세포개소2(IL-2)、y간우소(IFN-y)、종류배사인자α(TNF-α)、TNF-β、IL-4、IL-6、IL-8、IL-10화IL-17적표체수평.결과 해락인의뢰조혈청중Th1세포인자IL-2、IFN-γ、TNF-α화TNF-β수평균명현저우정상대조조[분별위(118±38) ng/L비(249±76) ng/L、(263±78) ng/L비(492±154) ng/L、(103±22) ng/L비(121±23) ng/L、(72±26) ng/L비(118±34) ng/L],차이균유통계학의의(t=3.154,t=4.518,t=2.459,t=3.089,균P＜0.05)；Th2세포인자IL-4、IL-6、IL-8、IL-10화IL-17적표체수평균명현고우정상대조조[분별위(252±72) ng/L비(124±32) ng/L、(4.4±1.6) ng/L비(2.8±1.0) ng/L、(409±112)ng/L비(214±67) ng/L、(12.1±3.4)ng/L비(8.2±2.0)ng/L、(164±50) ng/L비(95±28)ng/L],차이균유통계학의의(t=3.157,t =2.589,t=3.872,t=2.711,=3.255,균P＜0.05).결론 해락인의뢰자혈청중Th 1/Th2류세포인자수평교정상대조엄중실형,제시기면역공능교차,역획득외계감염,수급여족구중시.
Objective To research the expression levels of helper T cell 1 (Th1)/Th2 cytokines in heroin addicts,and to evaluate the immune status of heroin addicts.Methods Fifty cases of heroin addicts accommodated in Wenzhou detoxification from July 2012 to June 2013 were selected as the research objects.50 normal healthy people in the same period were selected as the control group.Interleukin 2 (IL-2),γinterferon (IFN-γ),tumor necrosis factor alpha (TNF alpha),TNF-3,IL-4,IL-6,IL-8,IL-10 and IL-17 expression levels in venous serum of the two groups were determined by radioimmunoassay.Results Th1 cytokines IL-2,IFN-γ,TNF-α and TNF-β level in serum of heroin-dependent group were significantly lower than those in the normal control group[(118 ± 38) ng/L vs (249 ± 76) ng/L,(263 ± 78) ng/L vs (492 ± 154) ng/L,(103 ± 22) ng/L vs (121 ± 23) ng/L,(72 ± 26) ng/L vs (118 ± 34) ng/L,respectively] ; the differences were statistically significant (t =3.154,t =4.518,t =2.459,t =3.089,all P ＜0.05); Th2 cytokines IL-4,IL-6,IL-8,IL-10 and IL-17 level in serum of heroin-dependent group were significantly higher than those in the normal control group [(252 ± 72) ng/L vs (124 ± 32) ng/L,(4.4 ± 1.6)ng/L vs (2.8 ± 1.0)ng/L,(409 ± 112)ng/L vs (214 ±67) ng/L,(12.1 ± 3.4) ng/L vs (8.2 ±2.0) ng/L,(164 ± 50) ng/L vs (95 ± 28) ng/L,respectively] ; the differences were statistically significant (t =3.157,t =2.589,t =3.872,t =2.711,t =3.255,all P ＜ 0.05).Conclusions Th1/Th2 cytokine levels in the serum of heroin addicts group are imbalanced and these people have poor immune function compared to normal control group.Heroin addicts is easy to get outside infections and need to be given adequate attention.
