中国医药
中國醫藥
중국의약
CHINA MEDICINE
2013年
z1期
47-48
,共2页
李弘%袁彩霞%秦琴%宋海霞%曹莹丽%孙喜娟
李弘%袁綵霞%秦琴%宋海霞%曹瑩麗%孫喜娟
리홍%원채하%진금%송해하%조형려%손희연
体外受精%鼠胚实验%质量控制%受精率%优胚率
體外受精%鼠胚實驗%質量控製%受精率%優胚率
체외수정%서배실험%질량공제%수정솔%우배솔
Vitro fertilization%Mouse embryo test%Quality control%Fertility rate%Grade 1-2 embryo rate
目的 利用小鼠体外受精技术对我院新建人类体外受精实验室进行质量控制.方法 手术获取小鼠配子,经体外受精或卵胞浆内单精子显微注射后,形成胚胎体外培养5d,观察受精率、优胚率、囊胚率.结果 本研究进行11个常规体外受精周期,取卵371枚,受精率82.2％(305枚),优胚率91.3％ (274枚),2-细胞囊胚形成率85.3％(256枚)；10个周期卵胞浆内单精子显微注射,取卵子206枚,受精率84.5％(174枚),优胚率92.9％(157枚),2-细胞囊胚形成率89.9％(152枚).结论 通过鼠胚实验对我科新建IVF实验室进行质量控制,结果符合标准.
目的 利用小鼠體外受精技術對我院新建人類體外受精實驗室進行質量控製.方法 手術穫取小鼠配子,經體外受精或卵胞漿內單精子顯微註射後,形成胚胎體外培養5d,觀察受精率、優胚率、囊胚率.結果 本研究進行11箇常規體外受精週期,取卵371枚,受精率82.2％(305枚),優胚率91.3％ (274枚),2-細胞囊胚形成率85.3％(256枚)；10箇週期卵胞漿內單精子顯微註射,取卵子206枚,受精率84.5％(174枚),優胚率92.9％(157枚),2-細胞囊胚形成率89.9％(152枚).結論 通過鼠胚實驗對我科新建IVF實驗室進行質量控製,結果符閤標準.
목적 이용소서체외수정기술대아원신건인류체외수정실험실진행질량공제.방법 수술획취소서배자,경체외수정혹란포장내단정자현미주사후,형성배태체외배양5d,관찰수정솔、우배솔、낭배솔.결과 본연구진행11개상규체외수정주기,취란371매,수정솔82.2％(305매),우배솔91.3％ (274매),2-세포낭배형성솔85.3％(256매)；10개주기란포장내단정자현미주사,취란자206매,수정솔84.5％(174매),우배솔92.9％(157매),2-세포낭배형성솔89.9％(152매).결론 통과서배실험대아과신건IVF실험실진행질량공제,결과부합표준.
Objective To carry out the quality control of new human in vitro fertilization (IVF) laboratory,IVF of mouse were studied.Methods The gametes of mouse were got by surgery,treated by IVF and intracytoplasmic sperm injection(ICSI),the embryos were cultured for five days in vitro,fertility rate,grade 1-2 embryo rate and rate of cleavage to blastulas were investigated.Results 11 cycles of IVF were finished,271 eggs were gained,the fertility rate was 82.2％ and grade 1-2 embryo rate was 91.3％,the rate of 2-cell was growing to blastulas was 85.3％.Ten cycle of ICSI were finished,206 oocytes at metaphase Ⅱ (M Ⅱ)were gained,the fertility rate was 84.5％ and grade 1-2 embryo rate was 92.9％,the rate of 2-cell was growing to blastulas was 89.9％.Conclusion The quality control of culture condition in human IVF technology is tested by mouse embryo,the data accord with standards.
