中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
2期
225-229
,共5页
实验性腹主动脉瘤%弹力蛋白酶%氯化钙%大鼠
實驗性腹主動脈瘤%彈力蛋白酶%氯化鈣%大鼠
실험성복주동맥류%탄력단백매%록화개%대서
Experimental abdominal aortic aneurysm%Elastase%Calcium chloride%Rat
目的 联合应用弹力蛋白酶和氯化钙浸润的方法建立病理学特征符合腹主动脉瘤特点的大鼠肾下型腹主动脉瘤模型.方法 选取雄性Sprague-Dawle大鼠的左肾动脉远心端1 cm腹主动脉段给予腔内弹力蛋白酶(40 U/ml)和腔外氯化钙(0.5 mol/L)处理(模型组,n=9),灌注组(n=6)(弹力蛋白酶和氯化钙溶液用0.9％氯化钠注射液代替)和假手术组(n=6)(仅正常开腹游离血管)作为对照组.分别于术前、术后3、7和10 d超声检测腹主动脉(瘤)直径变化.术后10 d取材固定,游标卡尺测量腹主动脉(瘤)直径,行组织切片苏木精-伊红染色和弹力蛋白Elastin yon Gieson(EVG)染色,计算机图像半定量分析比较3组血管壁厚度,弹力纤维含量参数的变化.结果 每组手术均在20 min内完成,围手术过程无技术失误和死亡.术后10 d,模型组腹主动脉直径以及扩张率分别为(2.75 ±0.49)mm、(68.4±22.6)％,高于灌注组[(1.86±0.16)mm,(6.3±3.7)％]和假手术组[(1.81±0.11)mm,(4.6±3.4)％].模型组、灌注组和假手术组术前、术后3、7和10 d动脉内径依时间变化分别为(1.68 ±0.18)mm,(2.37±0.26) mm,(2.69±0.34)mm,(2.81±0.38)mm;(1.71 ±0.15)mm,(1.79±0.21)mm,(1.83±0.19)mm,(1.82±0.24) mm;(1.66 0.19)mm,(1.64 0.14)mm,(1.63 ±0.22) mm,(1.61 ±0.13)mm.其中模型组动脉直径依时间变化明显,灌注组和假手术组2组无明显变化.苏木精-伊红染色示术后10 d模型组血管壁厚度为(136 ±6) μm明显小于灌注组的(177±5)μm和假手术组的(178±6)μm,灌注和假手术组2组差异无统计学意义(P＜0.05).弹力蛋白EVG染色示模型组弹力蛋白含量为(5.8±2.9)％明显低于灌注组的(17.1±6.4)％和假手术组的(16.7±7.1)％,上述结果灌注组和假手术组2组差异无统计学意义.结论 联合应用腔内弹力蛋白酶灌注和腔外氯化钙浸润处理可简单、有效地构建稳定性高、可复制性强的大鼠腹主动脉瘤模型,该模型在病理学特征上可有效模拟人腹主动脉瘤细胞外基质破坏特点,为腹主动脉瘤发病机制和干预治疗的研究提供良好的动物模型.
目的 聯閤應用彈力蛋白酶和氯化鈣浸潤的方法建立病理學特徵符閤腹主動脈瘤特點的大鼠腎下型腹主動脈瘤模型.方法 選取雄性Sprague-Dawle大鼠的左腎動脈遠心耑1 cm腹主動脈段給予腔內彈力蛋白酶(40 U/ml)和腔外氯化鈣(0.5 mol/L)處理(模型組,n=9),灌註組(n=6)(彈力蛋白酶和氯化鈣溶液用0.9％氯化鈉註射液代替)和假手術組(n=6)(僅正常開腹遊離血管)作為對照組.分彆于術前、術後3、7和10 d超聲檢測腹主動脈(瘤)直徑變化.術後10 d取材固定,遊標卡呎測量腹主動脈(瘤)直徑,行組織切片囌木精-伊紅染色和彈力蛋白Elastin yon Gieson(EVG)染色,計算機圖像半定量分析比較3組血管壁厚度,彈力纖維含量參數的變化.結果 每組手術均在20 min內完成,圍手術過程無技術失誤和死亡.術後10 d,模型組腹主動脈直徑以及擴張率分彆為(2.75 ±0.49)mm、(68.4±22.6)％,高于灌註組[(1.86±0.16)mm,(6.3±3.7)％]和假手術組[(1.81±0.11)mm,(4.6±3.4)％].模型組、灌註組和假手術組術前、術後3、7和10 d動脈內徑依時間變化分彆為(1.68 ±0.18)mm,(2.37±0.26) mm,(2.69±0.34)mm,(2.81±0.38)mm;(1.71 ±0.15)mm,(1.79±0.21)mm,(1.83±0.19)mm,(1.82±0.24) mm;(1.66 0.19)mm,(1.64 0.14)mm,(1.63 ±0.22) mm,(1.61 ±0.13)mm.其中模型組動脈直徑依時間變化明顯,灌註組和假手術組2組無明顯變化.囌木精-伊紅染色示術後10 d模型組血管壁厚度為(136 ±6) μm明顯小于灌註組的(177±5)μm和假手術組的(178±6)μm,灌註和假手術組2組差異無統計學意義(P＜0.05).彈力蛋白EVG染色示模型組彈力蛋白含量為(5.8±2.9)％明顯低于灌註組的(17.1±6.4)％和假手術組的(16.7±7.1)％,上述結果灌註組和假手術組2組差異無統計學意義.結論 聯閤應用腔內彈力蛋白酶灌註和腔外氯化鈣浸潤處理可簡單、有效地構建穩定性高、可複製性彊的大鼠腹主動脈瘤模型,該模型在病理學特徵上可有效模擬人腹主動脈瘤細胞外基質破壞特點,為腹主動脈瘤髮病機製和榦預治療的研究提供良好的動物模型.
목적 연합응용탄력단백매화록화개침윤적방법건립병이학특정부합복주동맥류특점적대서신하형복주동맥류모형.방법 선취웅성Sprague-Dawle대서적좌신동맥원심단1 cm복주동맥단급여강내탄력단백매(40 U/ml)화강외록화개(0.5 mol/L)처리(모형조,n=9),관주조(n=6)(탄력단백매화록화개용액용0.9％록화납주사액대체)화가수술조(n=6)(부정상개복유리혈관)작위대조조.분별우술전、술후3、7화10 d초성검측복주동맥(류)직경변화.술후10 d취재고정,유표잡척측량복주동맥(류)직경,행조직절편소목정-이홍염색화탄력단백Elastin yon Gieson(EVG)염색,계산궤도상반정량분석비교3조혈관벽후도,탄력섬유함량삼수적변화.결과 매조수술균재20 min내완성,위수술과정무기술실오화사망.술후10 d,모형조복주동맥직경이급확장솔분별위(2.75 ±0.49)mm、(68.4±22.6)％,고우관주조[(1.86±0.16)mm,(6.3±3.7)％]화가수술조[(1.81±0.11)mm,(4.6±3.4)％].모형조、관주조화가수술조술전、술후3、7화10 d동맥내경의시간변화분별위(1.68 ±0.18)mm,(2.37±0.26) mm,(2.69±0.34)mm,(2.81±0.38)mm;(1.71 ±0.15)mm,(1.79±0.21)mm,(1.83±0.19)mm,(1.82±0.24) mm;(1.66 0.19)mm,(1.64 0.14)mm,(1.63 ±0.22) mm,(1.61 ±0.13)mm.기중모형조동맥직경의시간변화명현,관주조화가수술조2조무명현변화.소목정-이홍염색시술후10 d모형조혈관벽후도위(136 ±6) μm명현소우관주조적(177±5)μm화가수술조적(178±6)μm,관주화가수술조2조차이무통계학의의(P＜0.05).탄력단백EVG염색시모형조탄력단백함량위(5.8±2.9)％명현저우관주조적(17.1±6.4)％화가수술조적(16.7±7.1)％,상술결과관주조화가수술조2조차이무통계학의의.결론 연합응용강내탄력단백매관주화강외록화개침윤처리가간단、유효지구건은정성고、가복제성강적대서복주동맥류모형,해모형재병이학특정상가유효모의인복주동맥류세포외기질파배특점,위복주동맥류발병궤제화간예치료적연구제공량호적동물모형.
Objective To introduce a new,simple technique using a combination of intraluminal elastaseinfusion and extraluminal calcium chloride exposure to set up abdominal aortic aneurysm (AAAs) that simulates human aneurysms.Methods Experimental models of AAAs were created in rats by a 20-minute application of intraluminal elastase (30U) and extraluminal calcium chloride (0.5 mol/L) in the 1 cm segment of infrarenal abdominal aorta (model group,n =9).A single application of elastase (Saline infusion group,n =6) or calcium chloride (sham group,n =6) was used as control.The aorta in each group was measured by ultrasonic detection before and after operation (3 d,7 d and 10 d) and was harvested at 10 d.Vascular wall thickness,lastolytic activity and histology in the treated aorta were evaluated among the three groups.Results The surgical procedure in each group was similarly completed for approximate 20 minutes and performed without any technical failure or operative death.At the 10th day,the artery diameter and the dilation ratio were (2.75 ± 0.49) mm and (68.4 ±22.6) ％ in model group,(1.86 ± 0.16) mm and (6.3 ± 3.7) ％ in saline infusion group,(1.81 ± 0.11) mm,(4.6± 3.4)％ in sham group,respectively.The wall thickness in model group(136 ± 6)μm was significantly higher than that in saline infusion group(177 ±5)pm and in sham group(178 ±6)μm.Less elastin content in the aortic wall was observed in model group.According to the time course of artery size measured by ultrasonic detection before and after operation (3,7 and 10 d),the artery diameter of the model group changed more significantly than other 2 groups.Conclusions The rat AAA model using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure is simple and easy to perform.It is highly reliable and reproducible to create a saccular aneurvsm similar to human AAAs.
