CAJ | 학술논문

目的探讨脂肪因子Apelin-13对3T3-L1脂肪细胞水通道蛋白7(AQP7)基因表达的影响.方法体外培养3T3-L1脂肪细胞,以油红O染色鉴定为成熟脂肪细胞后,分为阴性对照组(无干预),不同浓度(10-9、10-8、10-7、10-6nmol/L) Apelin-13干预组(均干预24 h),阳性对照组(10-5 nmol/L罗格列酮干预24 h)和10-7 nmol/L Apelin-13干预不同时间(0、12、24、36、48 h)组.用反转录-聚合酶链反应检测各组细胞AQP7 mRNA表达水平.结果阴性对照组、10-9、10-8、10-7、10-6 nmol/L Apelin-13和阳性对照组AQP7表达水平分别为(0.22±0.02)、(0.29±0.07)、(0.36±0.05)、(0.43±0.05)、(0.31±0.06)、(0.32±0.08),阳性对照组与阴性对照组之间差异有统计学意义(P＜0.05);与阴性对照组比较,10-8、10-7 nmol/L Apelin-13能明显刺激AQP7 mRNA表达(P ＜0.05);10-8、10-7 nmol/L Apelin-13组与阳性对照组比较,10-7 nmol/L Apelin-13能明显刺激AQP7 mRNA表达(P＜0.05);10-8、10-7 nmol/L Apelin-13组间差异无统计学意义.在时间组中,10-7 nmol/L Apelin-13的0、12、24、36、48 h各组灰度比值分别为(0.27±0.09)、(0.43±0.07)、(0.55±0.10)、(0.42±0.08)、(0.33±0.09),12、24、36 h组AQP7 mRNA表达较0h组能明显刺激AQP7 mRNA表达(P＜0.05),作用24 h表达最高;但12、24、36h3组之间AQP7mRNA表达差异无统计学意义.结论 Apelin-13在一定程度上能增加3T3-L1脂肪细胞AQP7 mRNA表达的水平,并分别以10-7 nmol/L和24 h为最佳作用浓度和时间.
목적 탐토지방인자Apelin-13대3T3-L1지방세포수통도단백7(AQP7)기인표체적영향.방법 체외배양3T3-L1지방세포,이유홍O염색감정위성숙지방세포후,분위음성대조조(무간예),불동농도(10-9、10-8、10-7、10-6nmol/L) Apelin-13간예조(균간예24 h),양성대조조(10-5 nmol/L라격렬동간예24 h)화10-7 nmol/L Apelin-13간예불동시간(0、12、24、36、48 h)조.용반전록-취합매련반응검측각조세포AQP7 mRNA표체수평.결과 음성대조조、10-9、10-8、10-7、10-6 nmol/L Apelin-13화양성대조조AQP7표체수평분별위(0.22±0.02)、(0.29±0.07)、(0.36±0.05)、(0.43±0.05)、(0.31±0.06)、(0.32±0.08),양성대조조여음성대조조지간차이유통계학의의(P＜0.05);여음성대조조비교,10-8、10-7 nmol/L Apelin-13능명현자격AQP7 mRNA표체(P ＜0.05);10-8、10-7 nmol/L Apelin-13조여양성대조조비교,10-7 nmol/L Apelin-13능명현자격AQP7 mRNA표체(P＜0.05);10-8、10-7 nmol/L Apelin-13조간차이무통계학의의.재시간조중,10-7 nmol/L Apelin-13적0、12、24、36、48 h각조회도비치분별위(0.27±0.09)、(0.43±0.07)、(0.55±0.10)、(0.42±0.08)、(0.33±0.09),12、24、36 h조AQP7 mRNA표체교0h조능명현자격AQP7 mRNA표체(P＜0.05),작용24 h표체최고;단12、24、36h3조지간AQP7mRNA표체차이무통계학의의.결론 Apelin-13재일정정도상능증가3T3-L1지방세포AQP7 mRNA표체적수평,병분별이10-7 nmol/L화24 h위최가작용농도화시간.
Objective To investigate a dependent manner of adipocykine Apelin-13 on Aquaporin (AQP7) gene expression in 3T3-L1 adipocyte.Methods The fully differentiated 3T3-L1 adipocytes identified by red oil O staining were divided into groups including negative control group,Apelin-13 treated groups with concentrations of 10-9,10-8,10-7,10-6 nmol/L for24 h and with concentration of 10-7 nmol/L for0,12,24,36 and 48 h and positive control group treated with rosiglitazone for 24 h with the concentration of 10-5 nmol/L.The expression of AQP7 mRNA was measured according to the reverse trans cription-polymerase reaction.Results The expressions of AQP7 mRNA in the negative control group,Apelin-13 treated groups with concentration of 10-9,10-8,10-7,10-6 nmol/L and positive control group were (0.22 ± 0.02),(0.29 ± 0.07),(0.36 ± 0.05),(0.43 ± 0.05),(0.31 ± 0.06) and (0.32 ± 0.08) respectively.There were significant differences between positive control group and negative control group (P ＜ 0.05); compared with negative control group,10 8,10 7 nmol/L Apelin-13 significantly stimulated AQP7 mRNA expression (P ＜ 0.05) ; 10 8,10-7 nmol/L Apelin-13 group compared with positive control group,10 7 nmol/L Apelin-13 significantly stimulated AQP7 mRNA expression (P ＜0.05); there showed no statistical difference between 10-8 and 10-7 nmol/L Apelin-13 group.In the time group,the garg-scale ratio of 0,12,24,36,48 h of 10-7 nmol/L Apelin-13 were (0.27 ±0.09),(0.43 ± 0.07),(0.55 ± 0.10),(0.42 ± 0.08),(0.33 ± 0.09) respectively.12,24,36 h group compared with 0 h group significantly stimulated AQP7 mRNA expression (P ＜ 0.05),and the highest expression at 24 h ; but there were no significant differences between the 12,24,36 h group.Conclusion To some extent,Apelin-13 increases AQP7 mRNA expression in 3T3-L1 adipocytes with the excellence concentration of 10-7 nmol/L treatment for 24 h.