中国医药
中國醫藥
중국의약
CHINA MEDICINE
2014年
5期
657-660
,共4页
邱晓伟%程世翔%涂悦%张赛
邱曉偉%程世翔%塗悅%張賽
구효위%정세상%도열%장새
颅脑创伤%应激性溃疡%动物模型%脑皮质损伤撞击仪
顱腦創傷%應激性潰瘍%動物模型%腦皮質損傷撞擊儀
로뇌창상%응격성궤양%동물모형%뇌피질손상당격의
Traumatic brain injury%Stress ulcer%Animal model%Cortical contusion impact
目的 应用电子脑皮质撞击仪(eCCI)建立颅脑创伤后应激性溃疡的动物模型,为进一步研究颅脑损伤所致应激性溃疡的发生机制及治疗方法奠定基础.方法 选取健康雄性Spraque-Dawley(SD)大鼠20只,按照随机数字表法分为假手术组和颅脑创伤组,每组10只.应用eCCI对颅脑创伤组大鼠顶叶大脑皮质区行精确撞击(打击速率4 m/s,打击时间200 ms,打击深度4 mm).假手术组仅用牙科钻开骨窗(5 mm ×5 mm),消毒后缝合头皮但不做打击.伤后48 h应用多普勒血流量计测定2组大鼠脑和胃黏膜表面局部血流量,苏木精-伊红染色观察脑和胃组织病理学改变.结果 致伤48 h后,颅脑创伤组脑组织血流量较假手术组明显下降[(40.2±1.9)ml/(min·100 g)比(107.6±3.4) ml/(min·100 g)](P ＜0.01).假手术组和颅脑创伤组胃黏膜表面血流量比较,差异有统计学意义[(32.9±2.0)ml/(min·100 g)比(42.1±1.0)ml/(min·100 g)](P＜0.01).致伤48 h后,光镜下假手术组大鼠脑组织正常,结构完整,胞核蓝染,胞质呈粉红色;颅脑创伤组大鼠脑组织损伤灶周围神经元数量明显减少,结构排列紊乱,局部脑组织变性坏死,逐渐形成瘢痕,伴大量红细胞渗出.假手术组大鼠胃组织基本正常,黏膜表层结构完整,黏膜下层未见明显嗜酸性炎细胞渗出;颅脑创伤组大鼠胃组织可见黏膜表层细胞、腺胃细胞变性、脱落,伴红细胞渗出,黏膜下可见嗜酸性炎细胞浸润.结论 大鼠颅脑创伤后存在应激性溃疡,应用脑皮质撞击仪可成功建立颅脑创伤后应激性溃疡的大鼠模型.
目的 應用電子腦皮質撞擊儀(eCCI)建立顱腦創傷後應激性潰瘍的動物模型,為進一步研究顱腦損傷所緻應激性潰瘍的髮生機製及治療方法奠定基礎.方法 選取健康雄性Spraque-Dawley(SD)大鼠20隻,按照隨機數字錶法分為假手術組和顱腦創傷組,每組10隻.應用eCCI對顱腦創傷組大鼠頂葉大腦皮質區行精確撞擊(打擊速率4 m/s,打擊時間200 ms,打擊深度4 mm).假手術組僅用牙科鑽開骨窗(5 mm ×5 mm),消毒後縫閤頭皮但不做打擊.傷後48 h應用多普勒血流量計測定2組大鼠腦和胃黏膜錶麵跼部血流量,囌木精-伊紅染色觀察腦和胃組織病理學改變.結果 緻傷48 h後,顱腦創傷組腦組織血流量較假手術組明顯下降[(40.2±1.9)ml/(min·100 g)比(107.6±3.4) ml/(min·100 g)](P ＜0.01).假手術組和顱腦創傷組胃黏膜錶麵血流量比較,差異有統計學意義[(32.9±2.0)ml/(min·100 g)比(42.1±1.0)ml/(min·100 g)](P＜0.01).緻傷48 h後,光鏡下假手術組大鼠腦組織正常,結構完整,胞覈藍染,胞質呈粉紅色;顱腦創傷組大鼠腦組織損傷竈週圍神經元數量明顯減少,結構排列紊亂,跼部腦組織變性壞死,逐漸形成瘢痕,伴大量紅細胞滲齣.假手術組大鼠胃組織基本正常,黏膜錶層結構完整,黏膜下層未見明顯嗜痠性炎細胞滲齣;顱腦創傷組大鼠胃組織可見黏膜錶層細胞、腺胃細胞變性、脫落,伴紅細胞滲齣,黏膜下可見嗜痠性炎細胞浸潤.結論 大鼠顱腦創傷後存在應激性潰瘍,應用腦皮質撞擊儀可成功建立顱腦創傷後應激性潰瘍的大鼠模型.
목적 응용전자뇌피질당격의(eCCI)건립로뇌창상후응격성궤양적동물모형,위진일보연구로뇌손상소치응격성궤양적발생궤제급치료방법전정기출.방법 선취건강웅성Spraque-Dawley(SD)대서20지,안조수궤수자표법분위가수술조화로뇌창상조,매조10지.응용eCCI대로뇌창상조대서정협대뇌피질구행정학당격(타격속솔4 m/s,타격시간200 ms,타격심도4 mm).가수술조부용아과찬개골창(5 mm ×5 mm),소독후봉합두피단불주타격.상후48 h응용다보륵혈류량계측정2조대서뇌화위점막표면국부혈류량,소목정-이홍염색관찰뇌화위조직병이학개변.결과 치상48 h후,로뇌창상조뇌조직혈류량교가수술조명현하강[(40.2±1.9)ml/(min·100 g)비(107.6±3.4) ml/(min·100 g)](P ＜0.01).가수술조화로뇌창상조위점막표면혈류량비교,차이유통계학의의[(32.9±2.0)ml/(min·100 g)비(42.1±1.0)ml/(min·100 g)](P＜0.01).치상48 h후,광경하가수술조대서뇌조직정상,결구완정,포핵람염,포질정분홍색;로뇌창상조대서뇌조직손상조주위신경원수량명현감소,결구배렬문란,국부뇌조직변성배사,축점형성반흔,반대량홍세포삼출.가수술조대서위조직기본정상,점막표층결구완정,점막하층미견명현기산성염세포삼출;로뇌창상조대서위조직가견점막표층세포、선위세포변성、탈락,반홍세포삼출,점막하가견기산성염세포침윤.결론 대서로뇌창상후존재응격성궤양,응용뇌피질당격의가성공건립로뇌창상후응격성궤양적대서모형.
Objective To establish of a traumatic brain injury-induced stress ulcer model in rats with an electric cortical contusion impactor (eCCI) for stress ulcer (SU).Methods A total of twenty Spraque-Dawley rats were selected and divided into sham group and brain injury group; each group had 10 cases.The parietal lobe brain cortexes in rats of brain injury group were given eCCI (4 m/s velocity for TBI,impact time was 200 ms,impact injury at 4 mm depth of penetration).Bone window of sham group was opened by dental drill (5 mm × 5 mm) after disinfection suture of the scalp.Regional blood flow of brain and gastric tissues in rats were determined by ultrasonic doppler blood flow meter after 48 hours of injury; histopathological changes of brain and gastric tissues were observed.Results Fourty eight hours after TBI,the mucosal blood flows in the brain surface in brain injury group were reduced compared with those in sham group (P ＜ 0.01) [(40.2 ± 1.9) ml/(min · 100 g) vs (107.6 ± 3.4) mL/(min · 100 g)].There was significant difference on regional blood flow between sham group and brain injury group(P＜0.01) [(32.9 ±2.0)ml/(min · 100 g) vs (42.1 ± 1.0)ml/(min · 100 g)].48 h after TBI,brain tissue of brain injury group was normal; structure was complete,the nucleus was stained blue and cytoplasm was pink.The number of neurons was decreased with structural disorder.The structure of gastric mucosal was disordered with mucosal surface cells and glandular stomach cell degeneration.Eosinophilic infiltrations of inflammatory cells in the sub mucosa of gastric were observed.Conclusions There is SU following TBI induced by electric cortical contusion impactor.The SU animal model induced by TBI in rats can be established successfully.
