中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
2期
109-112
,共4页
安英华%金英姬%方宇辉%金哲虎
安英華%金英姬%方宇輝%金哲虎
안영화%금영희%방우휘%금철호
瘢痕%瘦素%抗体%转化生长因子β1%兔
瘢痕%瘦素%抗體%轉化生長因子β1%兔
반흔%수소%항체%전화생장인자β1%토
Cicatrix%Leptin%Antibodies%Transforming growth factor β1%Rabbits
目的 探讨瘦素抗体对兔耳增生性瘢痕组织内转化生长因子(TGF)-β1mRNA表达的影响.方法 新西兰大白兔15只,每只大白兔双耳腹侧作直径约7 mm,深达软骨膜的圆形切口共6个,共获得兔耳增生性瘢痕模型90个,随机分为3组.生理氯化钠液组:生理氯化钠液外用40 d;瘦素抗体外用组:2 ng/ml瘦素抗体外用40 d;瘦素抗体外用+注射组(联合用药组):2 ng/ml瘦素抗体外用14 d后,每周注射1次2 ng/ml瘦素抗体,共3次.术后第40天各组均取瘢痕组织,计算瘢痕增生指数、组织病理学检查及测定瘢痕组织内TGF-β1mRNA表达.结果 瘦素抗体外用组和联合用药组瘢痕增生指数及瘢痕组织中TGF-β1 mRNA的表达明显下降,与生理氯化钠液组相比较,差异具有统计学意义,但两组之间比较,差异无统计学意义.组织病理学表明,生理氯化钠液组毛细血管增生明显,有大量成纤维细胞,核大、密集,排列不规则;瘦素抗体外用组与联合用药组成纤维细胞数量明显较少,核较小,排列相对规则.结论 瘦素抗体治疗能降低兔耳增生性瘢痕组织内的TGF-β1mRNA表达.
目的 探討瘦素抗體對兔耳增生性瘢痕組織內轉化生長因子(TGF)-β1mRNA錶達的影響.方法 新西蘭大白兔15隻,每隻大白兔雙耳腹側作直徑約7 mm,深達軟骨膜的圓形切口共6箇,共穫得兔耳增生性瘢痕模型90箇,隨機分為3組.生理氯化鈉液組:生理氯化鈉液外用40 d;瘦素抗體外用組:2 ng/ml瘦素抗體外用40 d;瘦素抗體外用+註射組(聯閤用藥組):2 ng/ml瘦素抗體外用14 d後,每週註射1次2 ng/ml瘦素抗體,共3次.術後第40天各組均取瘢痕組織,計算瘢痕增生指數、組織病理學檢查及測定瘢痕組織內TGF-β1mRNA錶達.結果 瘦素抗體外用組和聯閤用藥組瘢痕增生指數及瘢痕組織中TGF-β1 mRNA的錶達明顯下降,與生理氯化鈉液組相比較,差異具有統計學意義,但兩組之間比較,差異無統計學意義.組織病理學錶明,生理氯化鈉液組毛細血管增生明顯,有大量成纖維細胞,覈大、密集,排列不規則;瘦素抗體外用組與聯閤用藥組成纖維細胞數量明顯較少,覈較小,排列相對規則.結論 瘦素抗體治療能降低兔耳增生性瘢痕組織內的TGF-β1mRNA錶達.
목적 탐토수소항체대토이증생성반흔조직내전화생장인자(TGF)-β1mRNA표체적영향.방법 신서란대백토15지,매지대백토쌍이복측작직경약7 mm,심체연골막적원형절구공6개,공획득토이증생성반흔모형90개,수궤분위3조.생리록화납액조:생리록화납액외용40 d;수소항체외용조:2 ng/ml수소항체외용40 d;수소항체외용+주사조(연합용약조):2 ng/ml수소항체외용14 d후,매주주사1차2 ng/ml수소항체,공3차.술후제40천각조균취반흔조직,계산반흔증생지수、조직병이학검사급측정반흔조직내TGF-β1mRNA표체.결과 수소항체외용조화연합용약조반흔증생지수급반흔조직중TGF-β1 mRNA적표체명현하강,여생리록화납액조상비교,차이구유통계학의의,단량조지간비교,차이무통계학의의.조직병이학표명,생리록화납액조모세혈관증생명현,유대량성섬유세포,핵대、밀집,배렬불규칙;수소항체외용조여연합용약조성섬유세포수량명현교소,핵교소,배렬상대규칙.결론 수소항체치료능강저토이증생성반흔조직내적TGF-β1mRNA표체.
Objective To estimate the effect of leptin antibody on transforming growth factor (TGF)-β1 mRNA expression in hypertrophic scar model in rabbit ears.Methods Fifteen New Zealand white rabbits were included in this study.Three circular incisions which measured 7 mm in diameter and reached the perichondrium,were made in each ear of these rabbits to establish 90 models of hypertrophic scar.After the operation,these models were randomly and equally divided into 3 groups to be treated with topical sodium chloride physiological solution for 40 days (saline group),topical leptin antibody of 2 ng/ml for 40 days (leptin antibody group),and topical leptin antibody of 2 ng/ml for 14 days followed by injection of leptin antibody of 2 ng/ml once a week for 3 weeks (combination group).Scar tissue was resected from these rabbit ears at 40 days after the operation,followed by the determination of scar elevation index,histopathological examination by using haematoxylin and eosin staining,and quantification of TGF-β31 mRNA expression by real-time fluorescence-based PCR.SPSS 13.0 software was used for data processing.Statistical analysis was carried out by one-way analysis of variance.Results A significant decrease was observed in scar elevation index (2.33 ± 0.33 and 2.35 ± 0.22 vs.3.33 ± 0.41,both P ＜0.05) and TGF-β1 mRNA expression in the leptin antibody group and combination group compared with the control group,whereas no significant difference was observed between the leptin antibody group and combination group in either of the two parameters.Pathologically,there was an apparent proliferation of capillaries in the saline group with numerous irregularly and densely arranged fibroblasts with large nuclei,while relatively few fibroblasts with small nuclei,which were arranged in a more regular way,were observed in the leptin antibody group and combination group.Conclusion Leptin antibody treatment can reduce the expression of TGF-β1 mRNA in hypertrophic scar tissue in rabbit ears.
