CAJ | 학술논문

目的探讨成纤维细胞生长因子(FGF10)单克隆抗体局部外用对银屑病豚鼠模型的治疗作用.方法应用5％盐酸普萘洛尔搽剂外涂豚鼠耳背部皮肤,诱导银屑病样动物模型.分别设置空白组、模型组、丁酸氢化可的松治疗组、FGF10抗体高(0.188 g/L)、中(0.094 g/L)、低(0.063g/L)剂量治疗组.治疗2周后观察银屑病样模型的病理变化.采用SPSS 13.0统计软件进行统计学分析,Baker评分比较应用秩和检验,单一核细胞计数及表皮厚度比较应用方差分析(ANOVA),多个样本均数的两两比较应用最少显著差法(LSD).结果各治疗组上述各项检测指标均低于模型组(均P＜ 0.05).FGF10抗体高剂量组炎细胞计数与空白组差异无统计学意义(t=0.77,P＞0.05),其余各治疗组炎细胞计数高于空白组及FGF10抗体高剂量组(均P＜0.05).FGF10抗体各治疗组表皮厚度均高于丁酸氢化可的松治疗组,差异均有统计学意义(均P＜0.05).FGF10抗体各治疗组组间表皮厚度比较,差异无统计学意义(均P＞0.05).结论 FGF10单克隆抗体对银屑病样豚鼠模型中异常病理改变有显著调节作用,能影响角质形成细胞增殖分裂,还能显著抑制银屑病模型中炎症反应.
목적 탐토성섬유세포생장인자(FGF10)단극륭항체국부외용대은설병돈서모형적치료작용.방법 응용5％염산보내락이차제외도돈서이배부피부,유도은설병양동물모형.분별설치공백조、모형조、정산경화가적송치료조、FGF10항체고(0.188 g/L)、중(0.094 g/L)、저(0.063g/L)제량치료조.치료2주후관찰은설병양모형적병리변화.채용SPSS 13.0통계연건진행통계학분석,Baker평분비교응용질화검험,단일핵세포계수급표피후도비교응용방차분석(ANOVA),다개양본균수적량량비교응용최소현저차법(LSD).결과 각치료조상술각항검측지표균저우모형조(균P＜ 0.05).FGF10항체고제량조염세포계수여공백조차이무통계학의의(t=0.77,P＞0.05),기여각치료조염세포계수고우공백조급FGF10항체고제량조(균P＜0.05).FGF10항체각치료조표피후도균고우정산경화가적송치료조,차이균유통계학의의(균P＜0.05).FGF10항체각치료조조간표피후도비교,차이무통계학의의(균P＞0.05).결론 FGF10단극륭항체대은설병양돈서모형중이상병리개변유현저조절작용,능영향각질형성세포증식분렬,환능현저억제은설병모형중염증반응.
Objective To evaluate the therapeutic effect of local application of an anti-fibroblast growth factor 10 (FGF10) monoclonal antibody (MoAb) on a guinea pig model of psoriasis-like skin inflammation.Methods A model of psoriasis-like skin inflammation was established by applying 5％ propranolol hydrochloride emulsion to the dorsal skin of ears of 45 guinea pigs,which were then classified into 5 groups:model group receiving no treatment,hydrocortisone butyrate group,high-,medium-and low-dose MoAb groups receiving topical treatment with hydrocortisone butyrate,anti-FGF10 MoAb of 0.188 g/L,0.094 g/L and 0.063 g/L,respectively,twice daily.Nine guinea pigs receiving no propranolol challenge nor treatment served as the blank control group.The response of guinea pigs to propranolol hydrochloride emulsion and therapeutic agents,such as scratch behavior,hair status and skin status,was observed and recorded.After two weeks of treatment,all the guinea pigs were sacrificed,and skin samples were resected from the ears followed by the evaluation of Baker score,measurement of epidermal thickness,and enumeration of mononuclear cells.SPSS 13.0 software was used for data processing.Baker score was compared by rank sum test,mononuclear cell count and epidermal thickness by analysis of variance.Pairwise comparisons were carried out by the least significant difference (LSD) procedure.Results The number of mononuclear cells was similar between the high-dose MoAb group and blank control group (t =0.77,P ＞ 0.05),but higher in the other treatment groups than in the high-dose MoAb group and blank control group (all P ＜ 0.05).Increased epidermal thickness was observed in the three MoAb groups compared with the hydrocortisone butyrate group (all P ＜ 0.05),while there was no statistical difference between the three MoAb groups (all P ＞ 0.05).Conclusions The anti-FGF10 MoAb obviously attenuates the pathological changes,affects the proliferation of keratinocytes,and markedly suppresses the inflammation,in the psoriasis-like skin model in guinea pigs.