CAJ | 학술논문

目的鉴定并分析白念珠菌氟康唑敏感株与耐药株的差异表达膜蛋白质.方法以同一亲本来源的白念珠菌氟康唑敏感株CA-3和耐药株CA-16为研究对象,以双向聚丙烯酰胺凝胶电泳(2D-PAGE)技术分析菌株之间膜蛋白图谱的差异,以基质辅助激光解吸电离飞行时间串联质谱(MALDI-TOF/TOF-MS)鉴定表达差异的蛋白质,通过白念珠菌蛋白质数据库进行检索分析.结果白念珠菌氟康唑耐药株较敏感株有22种差异表达蛋白质点,其中有6种蛋白质上调,为Adh1p、Csp37p、Pgk1p、Pgk1p和2个未命名蛋白质(gi227305312、gi53954641);16种蛋白质下调,分别为Aco1p、Aco1p、Hsp78p、Gut2p、Sdh12p、Ilv2p、Ndh51p、Ndh51p、Atp1p、Pda1p、Srb1p、Idh1p、Tdh1p、Cyt1p、Cox4p、Cox13p.结论白念珠菌氟康唑耐药株较敏感株差异膜蛋白主要涉及能量代谢、线粒体功能等.
목적 감정병분석백념주균불강서민감주여내약주적차이표체막단백질.방법 이동일친본래원적백념주균불강서민감주CA-3화내약주CA-16위연구대상,이쌍향취병희선알응효전영(2D-PAGE)기술분석균주지간막단백도보적차이,이기질보조격광해흡전리비행시간천련질보(MALDI-TOF/TOF-MS)감정표체차이적단백질,통과백념주균단백질수거고진행검색분석.결과 백념주균불강서내약주교민감주유22충차이표체단백질점,기중유6충단백질상조,위Adh1p、Csp37p、Pgk1p、Pgk1p화2개미명명단백질(gi227305312、gi53954641);16충단백질하조,분별위Aco1p、Aco1p、Hsp78p、Gut2p、Sdh12p、Ilv2p、Ndh51p、Ndh51p、Atp1p、Pda1p、Srb1p、Idh1p、Tdh1p、Cyt1p、Cox4p、Cox13p.결론 백념주균불강서내약주교민감주차이막단백주요섭급능량대사、선립체공능등.
Objective To identify and analyze plasma membrane proteins differentially expressed between fluconazole-sensitive and-resistant C.albicans strains.Methods Two C.albicans strains from a same parent,including the fluconazole-sensitive C.albicans strain CA-3 and fluconazole-resistant C.albicans strain CA-16,served as the subject of this study.Plasma membrane proteins were isolated from both of the C.albicans strains,and subjected to two-dimensional polyacrylamide gel electrophoresis analysis for the screening of differentially expressed proteins,which were then identified by using matrix assisted laser desorption/ionization time-of-flight mass spectrometry.The resultant data were searched against a protein database for C.albicans.Results Twentytwo proteins were identified to be differentially expressed between the fiuconazole-resistant and-sensitive C.albicans strain.Of them,6 proteins (Adh1p,Csp37p,Pgk1p,Pgk1p and 2 unnamed proteins,i.e.,gi227305312and gi53954641) were highly expressed,while 16 proteins (Aco1p,Aco1p,Hsp78p,Gut2p,Sdh12p,Ilv2p,Ndh51p,Ndh51p,Atp1p,Pda1p,Srb1p,Idh1p,Tdh1p,Cyt1p,Cox4p,Cox13p) were lowly expressed in the fluconazole-resistant C.albicans strain compared with the fluconazole-sensitive strain.Conclusion The plasma membrane proteins differentially expressed between fluconazole-sensitive and-resistant C.albicans strain are mainly implicated in energy metabolism and mitochondrial function.