中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
6期
401-403
,共3页
祃丽娟%赵广%顾伟杰%牛建荣%邵丽芳%孟如松%闫文厅%成玉
祃麗娟%趙廣%顧偉傑%牛建榮%邵麗芳%孟如鬆%閆文廳%成玉
마려연%조엄%고위걸%우건영%소려방%맹여송%염문청%성옥
热%黑素细胞%HaCaT细胞%α促黑素
熱%黑素細胞%HaCaT細胞%α促黑素
열%흑소세포%HaCaT세포%α촉흑소
Heat%Melanocytes%HaCaT cells%α-MSH
目的 探讨热处理后体外培养的人表皮黑素细胞和人角质形成细胞(HaCaT细胞)分泌α黑素细胞刺激素(α-MSH)的变化.方法 将体外培养的黑素细胞及HaCaT细胞分别给予热处理(42℃,每天60 min,连续3d),实时荧光定量PCR法(RT-PCR)及酶联免疫吸附测定法(ELISA)检测两种细胞分泌的α-MSH的变化.结果 黑素细胞热处理组(1.5862±0.3262)与对照组(1.0000±0.2715)相比,α-MSH mRNA表达均明显增加(P＜0.05)；HaCaT细胞热处理组(1.8013±0.1216)与对照组(1.0000±0.2532)相比,mRNA水平也有明显增加(P＜0.05).ELISA结果:黑素细胞热处理组(0.3142±0.0469)蛋白表达水平明显高于对照组(0.2557±0.0330,P＜ 0.05)；HaCaT细胞热处理组(0.4632±0.0345)蛋白水平也明显高于对照组(0.4389±0.0399,P＜ 0.05).结论 热处理可促进人黑素细胞及HaCaT细胞α-MSH分泌.
目的 探討熱處理後體外培養的人錶皮黑素細胞和人角質形成細胞(HaCaT細胞)分泌α黑素細胞刺激素(α-MSH)的變化.方法 將體外培養的黑素細胞及HaCaT細胞分彆給予熱處理(42℃,每天60 min,連續3d),實時熒光定量PCR法(RT-PCR)及酶聯免疫吸附測定法(ELISA)檢測兩種細胞分泌的α-MSH的變化.結果 黑素細胞熱處理組(1.5862±0.3262)與對照組(1.0000±0.2715)相比,α-MSH mRNA錶達均明顯增加(P＜0.05)；HaCaT細胞熱處理組(1.8013±0.1216)與對照組(1.0000±0.2532)相比,mRNA水平也有明顯增加(P＜0.05).ELISA結果:黑素細胞熱處理組(0.3142±0.0469)蛋白錶達水平明顯高于對照組(0.2557±0.0330,P＜ 0.05)；HaCaT細胞熱處理組(0.4632±0.0345)蛋白水平也明顯高于對照組(0.4389±0.0399,P＜ 0.05).結論 熱處理可促進人黑素細胞及HaCaT細胞α-MSH分泌.
목적 탐토열처리후체외배양적인표피흑소세포화인각질형성세포(HaCaT세포)분비α흑소세포자격소(α-MSH)적변화.방법 장체외배양적흑소세포급HaCaT세포분별급여열처리(42℃,매천60 min,련속3d),실시형광정량PCR법(RT-PCR)급매련면역흡부측정법(ELISA)검측량충세포분비적α-MSH적변화.결과 흑소세포열처리조(1.5862±0.3262)여대조조(1.0000±0.2715)상비,α-MSH mRNA표체균명현증가(P＜0.05)；HaCaT세포열처리조(1.8013±0.1216)여대조조(1.0000±0.2532)상비,mRNA수평야유명현증가(P＜0.05).ELISA결과:흑소세포열처리조(0.3142±0.0469)단백표체수평명현고우대조조(0.2557±0.0330,P＜ 0.05)；HaCaT세포열처리조(0.4632±0.0345)단백수평야명현고우대조조(0.4389±0.0399,P＜ 0.05).결론 열처리가촉진인흑소세포급HaCaT세포α-MSH분비.
Objetive To evaluate the in vitro effect of heat treatment on the secretion of α-melanocytestimulating hormone (α-MSH) by human melanocytes and HaCaT keratinocytes.Methods Cultured normal human melanocytes from forskin tissue and HaCaT cells were treated with hyperthermia at 42 ℃ for 1 hour per day for 3 days.Subsequently,real time fluorescence-based quantitative PCR and enzyme-linked immunosorbent assay (ELISA) were performed to detect the mRNA and protein expressions of α-MSH,respectively.Results The mRNA expression level of α-MSH was significantly higher in heat-treated melanocytes and HaCaT cells than in those untreated (1.5862 ± 0.3262 vs.1.0000 ± 0.2715,P ＜ 0.05; 1.8013 ± 0.1216 vs.1.0000 ± 0.2532,P ＜ 0.05).Moreover,ELISA showed a statistical increase in the supematant level of α-MSH protein in heat-treated melanocytes and HaCaT cells compared with those untreated (0.3142 ± 0.0469 vs.0.2557 ± 0.0330,P ＜ 0.05; 0.4632 ± 0.0345 vs.0.4389 ± 0.0399,P ＜ 0.05).Conclusion Heat treatment can promote the secretion of α-MSH by melanocytes and HaCaT cells.
