CAJ | 학술논문

目的探讨BIOMED-2系统T细胞受体(TCR)γ引物组合在蕈样肉芽肿(MF)患者不同来源标本中TCRγ基因重排检测中的价值.方法收集28例MF患者15份石蜡组织样本、14份新鲜皮损及18份全血组织样本,提取DNA,利用BIOMED-2系统TCRγ引物组合进行TCRγ基因重排检测,比较3组不同来源样本的检出率并进行统计学分析.用SPSS13.0软件进行统计分析.各组之间阳性率采用x2检验或Fisher确切概率法检验.结果 15份石蜡包埋组织标本3份TCRγ基因重排阳性,18份血液标本11份阳性,14份新鲜皮损标本12份阳性,3组之间阳性率差异有统计学意义(x2=13.047,P＜ 0.01),新鲜皮损TCRγ基因重排阳性率显著高于石蜡组织.201 1年的6例石蜡标本3份基因重排阳性,阳性率显著高于其余9例2011年之前的石蜡标本(Fisher精确概率法,P=0.044),与14例新鲜组织标本TCRγ基因重排阳性率(12/14)相比差异无统计学意义(Fisher精确概率法,P=0.131).血液标本TCRγ基因重排阳性率低于新鲜皮损,两组之间差异无统计学意义(x2=2.358,P＞0.05).结论 BIOMED-2系统TCRγ引物组合适用于检测MF患者不同组织样本的TCR基因重排,更适用于新鲜的组织标本.
목적 탐토BIOMED-2계통T세포수체(TCR)γ인물조합재심양육아종(MF)환자불동래원표본중TCRγ기인중배검측중적개치.방법 수집28례MF환자15빈석사조직양본、14빈신선피손급18빈전혈조직양본,제취DNA,이용BIOMED-2계통TCRγ인물조합진행TCRγ기인중배검측,비교3조불동래원양본적검출솔병진행통계학분석.용SPSS13.0연건진행통계분석.각조지간양성솔채용x2검험혹Fisher학절개솔법검험.결과 15빈석사포매조직표본3빈TCRγ기인중배양성,18빈혈액표본11빈양성,14빈신선피손표본12빈양성,3조지간양성솔차이유통계학의의(x2=13.047,P＜ 0.01),신선피손TCRγ기인중배양성솔현저고우석사조직.201 1년적6례석사표본3빈기인중배양성,양성솔현저고우기여9례2011년지전적석사표본(Fisher정학개솔법,P=0.044),여14례신선조직표본TCRγ기인중배양성솔(12/14)상비차이무통계학의의(Fisher정학개솔법,P=0.131).혈액표본TCRγ기인중배양성솔저우신선피손,량조지간차이무통계학의의(x2=2.358,P＞0.05).결론 BIOMED-2계통TCRγ인물조합괄용우검측MF환자불동조직양본적TCR기인중배,경괄용우신선적조직표본.
Objective To estimate the value of BIOMED-2 primers for the detection of T cell receptor γ (TCR-γ) gene rearrangements in different types of specimens from patients with mycosis fungoides (MF).Methods Totally,15 paraffin-embedded tissue specimens,14 fresh tissue specimens and 18 whole blood specimens were obtained from 28 patients with MF,and subjected to DNA extraction.BIOMED-2 multiplex PCR tubes TCRγ (A+B) were used for the analysis of TCRγgene rearrangements.Data were processed by SPSS 13.0 software,and statistical analysis was done by chi-square test and Fisher's exact probability test.Results TCR-γ gene rearrangements were detected in 3 paraffin-embedded tissue specimens,11 fresh tissue specimens and 12 blood specimens,with significant differences in the detection rate between the three samples (x2 =13.047,P ＜ 0.01).The fresh tissue samples showed a significantly higher detection rate than the paraffin-embedded tissue samples (X2 =12.523,P ＜ 0.01).The detection rate of TCRγgene rearrangements was 3/6 in paraffin-embedded tissue samples collected in 2011,significantly higher than that in the other 9 paraffin-embedded tissue samples collected before 2011 (Fisher's exact probability test,P =0.044),but similar to that in 14 fresh tissue specimens (12/14,Fisher's exact probability test,P =0.044).Decreased detection rate of TCRγ gene rearrangements was observed in blood samples compared with fresh tissue specimens,but no statistical difference was observed between the two types of specimens (x2 =2.358,P ＞ 0.05).Conclusions BIOMED-2 multiplex PCR tubes TCRγ(A+B) are suitable for the detection of clonal rearrangements of TCRγgene in different types of specimens,especially in fresh tissue specimens,from patients with MF.