中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
6期
411-414
,共4页
许发明%许爱娥%吴纪龙%欧阳杰
許髮明%許愛娥%吳紀龍%歐暘傑
허발명%허애아%오기룡%구양걸
白癜风%CD8阳性T淋巴细胞%肿瘤坏死因子α%干扰素Ⅱ型%受体,白细胞介素2%茶多酚
白癜風%CD8暘性T淋巴細胞%腫瘤壞死因子α%榦擾素Ⅱ型%受體,白細胞介素2%茶多酚
백전풍%CD8양성T림파세포%종류배사인자α%간우소Ⅱ형%수체,백세포개소2%다다분
Vitiligo%CD8-positive T-lymphocytes%Tumor necrosis factor alpha%Interferon type Ⅱ%Receptors,interleukin-2%Tea polyphenols
目的 检测CD8+T淋巴细胞分泌细胞因子及受体在白癜风患者外周血中的表达情况及意义,并探讨茶多酚对其表达的影响.方法 分离和培养12例进展期白癜风、12例稳定期患者和10例健康人外周血CD8+T淋巴细胞,与茶多酚100 mg/L共培养,酶联免疫吸附试验(ELISA)检测茶多酚处理前后肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ和白介素-2受体(IL-2R)的浓度.用t检验和方差分析检测不同组间及处理前后各因子水平的差异.结果 进展期白癜风组、稳定期白癜风组和健康对照组外周血CD8+T淋巴细胞分泌TNF-α的浓度依次降低,分别为(191.302±6.077) ng/L、(175.966±2.467) ng/L、(173.664±3.600) ng/L,IFN-γ的浓度依次升高,分别为(280.182±36.07) ng/L、(371.670±24.352)ng/L、(447.147±8.432)ng/L,IL-2R的浓度同样依次升高,分别为(8.375±0.161) μg/L、(8.845±0.161) μg/L、(9.345±0.125) μg/L.进展期白癜风组与稳定期白癜风组和健康对照组相比较,3种指标差异均有统计学意义(P＜ 0.05或0.01).经茶多酚处理2d后,进展期白癜风组、稳定期白癜风组和健康对照组TNF-α水平均明显降低至(164.797±1.784) ng/L、(166.150±3.576) ng/L、(155.028±5.759) ng/L,与处理前比较差异均有统计学意义(均P＜0.05)；稳定期白癜风组IFN-γ浓度升高,而进展期白癜风组和健康对照组则有降低,各组IL-2R水平均有升高,但差异均无统计学意义(均P＞ 0.05).结论 白癜风患者外周血CD8+T淋巴细胞分泌的细胞因子及受体的变化可能与白癜风诱发或进展相关.茶多酚可能通过降低CD8+T淋巴细胞分泌TNF-α治疗白癜风.
目的 檢測CD8+T淋巴細胞分泌細胞因子及受體在白癜風患者外週血中的錶達情況及意義,併探討茶多酚對其錶達的影響.方法 分離和培養12例進展期白癜風、12例穩定期患者和10例健康人外週血CD8+T淋巴細胞,與茶多酚100 mg/L共培養,酶聯免疫吸附試驗(ELISA)檢測茶多酚處理前後腫瘤壞死因子(TNF)-α、榦擾素(IFN)-γ和白介素-2受體(IL-2R)的濃度.用t檢驗和方差分析檢測不同組間及處理前後各因子水平的差異.結果 進展期白癜風組、穩定期白癜風組和健康對照組外週血CD8+T淋巴細胞分泌TNF-α的濃度依次降低,分彆為(191.302±6.077) ng/L、(175.966±2.467) ng/L、(173.664±3.600) ng/L,IFN-γ的濃度依次升高,分彆為(280.182±36.07) ng/L、(371.670±24.352)ng/L、(447.147±8.432)ng/L,IL-2R的濃度同樣依次升高,分彆為(8.375±0.161) μg/L、(8.845±0.161) μg/L、(9.345±0.125) μg/L.進展期白癜風組與穩定期白癜風組和健康對照組相比較,3種指標差異均有統計學意義(P＜ 0.05或0.01).經茶多酚處理2d後,進展期白癜風組、穩定期白癜風組和健康對照組TNF-α水平均明顯降低至(164.797±1.784) ng/L、(166.150±3.576) ng/L、(155.028±5.759) ng/L,與處理前比較差異均有統計學意義(均P＜0.05)；穩定期白癜風組IFN-γ濃度升高,而進展期白癜風組和健康對照組則有降低,各組IL-2R水平均有升高,但差異均無統計學意義(均P＞ 0.05).結論 白癜風患者外週血CD8+T淋巴細胞分泌的細胞因子及受體的變化可能與白癜風誘髮或進展相關.茶多酚可能通過降低CD8+T淋巴細胞分泌TNF-α治療白癜風.
목적 검측CD8+T림파세포분비세포인자급수체재백전풍환자외주혈중적표체정황급의의,병탐토다다분대기표체적영향.방법 분리화배양12례진전기백전풍、12례은정기환자화10례건강인외주혈CD8+T림파세포,여다다분100 mg/L공배양,매련면역흡부시험(ELISA)검측다다분처리전후종류배사인자(TNF)-α、간우소(IFN)-γ화백개소-2수체(IL-2R)적농도.용t검험화방차분석검측불동조간급처리전후각인자수평적차이.결과 진전기백전풍조、은정기백전풍조화건강대조조외주혈CD8+T림파세포분비TNF-α적농도의차강저,분별위(191.302±6.077) ng/L、(175.966±2.467) ng/L、(173.664±3.600) ng/L,IFN-γ적농도의차승고,분별위(280.182±36.07) ng/L、(371.670±24.352)ng/L、(447.147±8.432)ng/L,IL-2R적농도동양의차승고,분별위(8.375±0.161) μg/L、(8.845±0.161) μg/L、(9.345±0.125) μg/L.진전기백전풍조여은정기백전풍조화건강대조조상비교,3충지표차이균유통계학의의(P＜ 0.05혹0.01).경다다분처리2d후,진전기백전풍조、은정기백전풍조화건강대조조TNF-α수평균명현강저지(164.797±1.784) ng/L、(166.150±3.576) ng/L、(155.028±5.759) ng/L,여처리전비교차이균유통계학의의(균P＜0.05)；은정기백전풍조IFN-γ농도승고,이진전기백전풍조화건강대조조칙유강저,각조IL-2R수평균유승고,단차이균무통계학의의(균P＞ 0.05).결론 백전풍환자외주혈CD8+T림파세포분비적세포인자급수체적변화가능여백전풍유발혹진전상관.다다분가능통과강저CD8+T림파세포분비TNF-α치료백전풍.
Objective To detect the level of cytokines and their receptors secreted by peripheral blood CD8 + T lymphocytes from vitiligo patients,and to evaluate the influence of tea polyphenols on their secretion.Methods CD8+ T lymphocytes were isolated from the peripheral blood of 12 patients with progressive vitiligo,12 patients with stable vitiligo and 10 healthy controls,and cultured in vitro for 20 days.Then,some CD8+ T lymphocytes were treated with tea polyphenols of 100 mg/L for two days.Enzyme-linked immunosorbent assay (ELISA) was carried out to determine the levels of tumor necrosis factor (TNF)-α,interferon (IFN)-γ and interleukin-2 receptor (IL-2R) in the culture supernatants of CD8+ T lymphocytes before and after the treatment with tea polyphenols.Analysis of variance and t test were conducted to assess differences in these parameters among these groups and changes between pretreatment and posttreatment,respectively.Results There was a sequential decrease in the level of TNF-α ((191.302 ± 6.077) vs.(175.966 ± 2.467) vs.(173.664 ± 3.600) ng/L,F =4.784,P ＜ 0.05),but a sequential increase in that of IFN-γ ((280.182 ± 36.070) vs.(371.670 ± 24.352) vs.(447.147 ± 8.432) ng/L,F=9.036,P＜ 0.01) and IL-2R ((8.375 ± 0.161) vs.(8.845 ± 0.161) vs.(9.345 ±0.125) ng/L,F =9.639,P ＜ 0.01) in the culture supernatant of CD8+ T lymphocytes from patients with progressive vitiligo to patients with stable vitiligo and healthy controls.After two days of tea polyphenol treatment,a statistical decrease was observed in the level of TNF-α in the culture supernatant of CD8 + T lymphocytes from patients with progressive vitiligo ((164.797 ± 1.784) ng/L,P ＜ 0.01),patients with stable vitiligo ((166.150 ± 3.576) ng/L,P ＜ 0.05) and healthy controls ((155.028 ± 5.759) ng/L,P ＜ 0.05),but no statistical changes were noted for IFN-γor IL-2R (all P ＞ 0.05) despite a slight elevation in the level of IFN-γin the culture supernatant of CD8+ T lymphocytes from patients with stable vitiligo and in that of IL-2R from all the three groups as well as a mild reduction in that of IFN-γ from patients with progressive vitiligo and healthy controls.Conclusions The changes of cytokines and their receptors secreted by CD8 + T lymphocytes may be associated with the induction and progression of vitiligo.Tea polyphenols may treat vitiligo via suppressing the secretion of TNF-α by CD8+ T lymphocytes.
