中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
7期
457-461
,共5页
王亚哲%张嵩%郑姝颖%江滨%刘艳荣%陈珊珊%路瑾
王亞哲%張嵩%鄭姝穎%江濱%劉豔榮%陳珊珊%路瑾
왕아철%장숭%정주영%강빈%류염영%진산산%로근
白血病,髓样%树突细胞%浆细胞
白血病,髓樣%樹突細胞%漿細胞
백혈병,수양%수돌세포%장세포
Leukemia,myeloid%Dendritic cells%Plasma cells
患者女,19岁,左下肢暗红色斑丘疹1年余,右腹股沟可触及一樱桃大小淋巴结.皮肤肿物组织病理检查:皮肤正常结构消失,可见淋巴样肿瘤细胞弥漫浸润,细胞中等大小,核仁不清楚;免疫组化:CD4(++)、CD56(++)、CD123(+++),TdT部分阳性.骨髓穿刺行流式细胞检查:异常细胞占59.9%,表达CD123st(IL-3Ra)、HLA-DRst、CD56、CD304 (BDCA-4)、CD7、CD11b、CD33、CD36、CXCR4和CD13dim,部分细胞表达CD4和CD117.透射电镜观察骨髓细胞:可见大量形态大小均一的淋巴样细胞,细胞表面可见粗大突起,胞核胞质比例高,胞核圆形居中,有呈脑回样深切迹,异染色质块状边集,核仁多见,少量胞质,其中可见内质网、线粒体等细胞器,内质网均呈浆细胞样同心圆状排列.骨髓细胞表现兼具树突细胞及浆细胞特征.诊断:母细胞性浆细胞样树突细胞肿瘤.
患者女,19歲,左下肢暗紅色斑丘疹1年餘,右腹股溝可觸及一櫻桃大小淋巴結.皮膚腫物組織病理檢查:皮膚正常結構消失,可見淋巴樣腫瘤細胞瀰漫浸潤,細胞中等大小,覈仁不清楚;免疫組化:CD4(++)、CD56(++)、CD123(+++),TdT部分暘性.骨髓穿刺行流式細胞檢查:異常細胞佔59.9%,錶達CD123st(IL-3Ra)、HLA-DRst、CD56、CD304 (BDCA-4)、CD7、CD11b、CD33、CD36、CXCR4和CD13dim,部分細胞錶達CD4和CD117.透射電鏡觀察骨髓細胞:可見大量形態大小均一的淋巴樣細胞,細胞錶麵可見粗大突起,胞覈胞質比例高,胞覈圓形居中,有呈腦迴樣深切跡,異染色質塊狀邊集,覈仁多見,少量胞質,其中可見內質網、線粒體等細胞器,內質網均呈漿細胞樣同心圓狀排列.骨髓細胞錶現兼具樹突細胞及漿細胞特徵.診斷:母細胞性漿細胞樣樹突細胞腫瘤.
환자녀,19세,좌하지암홍색반구진1년여,우복고구가촉급일앵도대소림파결.피부종물조직병리검사:피부정상결구소실,가견림파양종류세포미만침윤,세포중등대소,핵인불청초;면역조화:CD4(++)、CD56(++)、CD123(+++),TdT부분양성.골수천자행류식세포검사:이상세포점59.9%,표체CD123st(IL-3Ra)、HLA-DRst、CD56、CD304 (BDCA-4)、CD7、CD11b、CD33、CD36、CXCR4화CD13dim,부분세포표체CD4화CD117.투사전경관찰골수세포:가견대량형태대소균일적림파양세포,세포표면가견조대돌기,포핵포질비례고,포핵원형거중,유정뇌회양심절적,이염색질괴상변집,핵인다견,소량포질,기중가견내질망、선립체등세포기,내질망균정장세포양동심원상배렬.골수세포표현겸구수돌세포급장세포특정.진단:모세포성장세포양수돌세포종류.
A 19-year-old female presented with a dark erythematous maculopapule measuring 2 cm in diameter on the left lower limb for more than one year.Physical examination revealed an enlarged cherry-like lymph node in the right inguinal region.Histopathology of the lesion revealed that the normal structure of skin disappeared absolutely,and there was a diffuse infiltrate of medium-sized lymphoid tumor cells with unclear nucleoli.Immunohistochemically,the tumor cells strongly expressed CD4,CD56 and CD123,and partially expressed terminal deoxynucleotidyl transferase (TdT).Flow cytometric analysis of bone marrow aspirates showed that abnormal cells amounted to 59.9%,and were positive for CD123st (strong),human leucocyte antigen-DRst (strong),CD56,CD304 (blood dendritic cell antigen-4),CD7,CD11b,CD33,CD36 and chemokine (C-X-C motif) receptor 4,CD13 (dim),CD4 (partial) and CD117 (partial).Transmission electron microscopy of bone marrow cells revealed that there were massive uniformly sized lymphoid cells with thick processes on cell surfaces,a high nucleus/cytoplasm ratio,centrally located round nuclei occasionally with deep gyrus-like notches,clumped marginated heterochromatin and multiple nucleoli.Moreover,a small amount of cytoplasm was observed in the lymphoid cells with the presence of mitochondria and endoplasmic reticulum arranged in concentric circles,which were characteristic of plasma cells.The features of both dendritic cells and plasma cells were found in the tumor cells through transmission electron microscopy.Based on the above findings,a diagnosis of blastic plasmacytoid dendritic cell neoplasm (BPDCN) was made.