CAJ | 학술논문

目的探讨特应性皮炎(AD)患者外周血调节性T细胞(Treg细胞)及皮损组织中miR-31、Foxp3的表达水平及其相关性.方法用流式细胞仪检测37例AD患者外周血Treg细胞的比例,实时荧光定量RT-PCR检测miR-31及Treg细胞特异性转录因子Foxp3 mRNA的表达水平；并以33例性别、年龄匹配的健康儿童做对照.检测5例急性期AD患者皮损与皮损周围组织中miR-31及Foxp3 mRNA的表达水平,并以5例健康人皮肤组织标本为对照.采用独立样本t检验、单因素方差分析和线性相关进行统计学分析.结果 AD患者外周血Treg细胞比例明显低于健康对照组(2.12％±0.60％比4.99％±1.27％,P＜0.01)；miR-31 mRNA表达水平明显高于健康对照组(6.01±1.76比1.62±0.51,P＜0.01)；Foxp3 mRNA表达水平显著低于健康对照组(0.78±0.17比1.87±0.71,P＜0.01).AD患者皮损、皮损周围组织及健康人皮肤组织中miR-31及Foxp3 mRNA表达水平间差异均有统计学意义.miR-31表达水平:皮损＞皮损周围组织＞健康人皮肤组织,F=54.501,P＜0.01;Foxp3表达水平:皮损＜皮损周围组织＜健康人皮肤组织,F=37.837,P＜0.01.AD患者外周血miR-31 mRNA表达水平与疾病严重程度评分呈正相关(r=0.417,P=0.010),与Treg细胞比例(r=-0.404,P=0.013)及Foxp3 mRNA表达水平呈负相关(r=-0.409,P=0.012)；皮损及皮损周围组织中miR-31 mRNA表达水平与Foxp3 mRNA表达水平呈负相关(r=-0.392,P=0.032).结论 AD患者miR-31高表达及Foxp3低表达可能与AD的发病相关.
목적 탐토특응성피염(AD)환자외주혈조절성T세포(Treg세포)급피손조직중miR-31、Foxp3적표체수평급기상관성.방법 용류식세포의검측37례AD환자외주혈Treg세포적비례,실시형광정량RT-PCR검측miR-31급Treg세포특이성전록인자Foxp3 mRNA적표체수평；병이33례성별、년령필배적건강인동주대조.검측5례급성기AD환자피손여피손주위조직중miR-31급Foxp3 mRNA적표체수평,병이5례건강인피부조직표본위대조.채용독립양본t검험、단인소방차분석화선성상관진행통계학분석.결과 AD환자외주혈Treg세포비례명현저우건강대조조(2.12％±0.60％비4.99％±1.27％,P＜0.01)；miR-31 mRNA표체수평명현고우건강대조조(6.01±1.76비1.62±0.51,P＜0.01)；Foxp3 mRNA표체수평현저저우건강대조조(0.78±0.17비1.87±0.71,P＜0.01).AD환자피손、피손주위조직급건강인피부조직중miR-31급Foxp3 mRNA표체수평간차이균유통계학의의.miR-31표체수평:피손＞피손주위조직＞건강인피부조직,F=54.501,P＜0.01;Foxp3표체수평:피손＜피손주위조직＜건강인피부조직,F=37.837,P＜0.01.AD환자외주혈miR-31 mRNA표체수평여질병엄중정도평분정정상관(r=0.417,P=0.010),여Treg세포비례(r=-0.404,P=0.013)급Foxp3 mRNA표체수평정부상관(r=-0.409,P=0.012)；피손급피손주위조직중miR-31 mRNA표체수평여Foxp3 mRNA표체수평정부상관(r=-0.392,P=0.032).결론 AD환자miR-31고표체급Foxp3저표체가능여AD적발병상관.
Objective To determine the proportion of peripheral blood regulatory T (Treg) cells as well as mRNA expressions of miR-31 and Foxp3 in peripheral blood and skin lesions from patients with atopic dermatitis (AD),and to assess their association.Methods Peripheral blood samples were obtained from 37patients with AD and 33 age-and sex-matched healthy controls,and biopsy samples from the lesional and perilesional skin of 5 patients with AD as well as from the normal skin of 5 healthy controls.Flow cytometry was performed to determine the percentage of Treg cells (CD4+CD25+Foxp3+ T cells) in peripheral blood,and fluorescence-based real time quantitative reverse transcription (RT)-PCR to quantify the mRNA expressions of miR-31 and Foxp3 (a Treg cell-specific transcription factor) in peripheral blood and skin samples.Independent samples t test was carried out to compare the percentage of Treg cells as well as the mRNA expressions of miR31 and Foxp3 in peripheral blood between the patients and controls,one-way analysis of variance to compare the mRNA expressions of miR-31 and Foxp3 in biopsy samples between the patients and controls,and Pearson correlation analysis to evaluate the relationship between miR-31 mRNA expression level,SCORing Atopic Dermatitis (SCORAD) score,Treg cell percentage,and Foxp3 mRNA expression level.Results The patients with AD showed decreased Treg cell percentage (2.12％ ± 0.60％ vs.4.99％ ± 1.27％,P ＜0.01) and Foxp3 mRNA expression level (0.78 ± 0.17 vs.1.87 ± 0.71,P ＜0.01),but increased miR-31 mRNA expression level (6.01 ± 1.76 vs.1.62 ± 0.51,P ＜0.01)in peripheral blood compared with the healthy controls.The expression level of miR-31 mRNA sequentially decreased (F =54.501,P ＜0.01),but the expression of Foxp3 mRNA sequentially increased (F =37.837,P ＜0.01) from lesional skin,perilesional skin to normal skin tissues.The miR-31 mRNA expression level was positively correlated with SCORAD score (r =0.417,P =0.010),but negatively correlated with Treg cell percentage (r =-0.404,P =0.013) and Foxp3 mRNA level (r =-0.409,P=0.012) in peripheral blood of patients with AD.There was a negative correlation between the mRNA expression level of miR-31 and Foxp3 in lesional and peri-lesional skin tissues of the patients (r =-0.392,P =0.032).Conclusion The upregulated miR-31 expression and downregulated Foxp3 expression may be associated with the development of AD.