中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
7期
475-479
,共5页
瘢痕疙瘩%半乳糖β-1,4-糖苷键%胶原Ⅰ型%蓖麻蛋白
瘢痕疙瘩%半乳糖β-1,4-糖苷鍵%膠原Ⅰ型%蓖痳蛋白
반흔흘탑%반유당β-1,4-당감건%효원Ⅰ형%비마단백
Keloid%Galβ-1,4GlcNAc%Collagen type Ⅰ%Ricin
目的 探讨半乳糖β-1,4-糖苷键在瘢痕疙瘩组织中的合成、定位及糖蛋白半乳糖基化在瘢痕疙瘩形成机制中的作用.方法 Lectin印迹法观察瘢痕疙瘩、增生性瘢痕和正常皮肤组织中糖蛋白的糖基化水平；饱和苦味酸-天狼猩红偏振光法观察组织学结构及胶原的形态与分布.分析组织中Ⅰ、Ⅲ型胶原的比例；蓖麻凝集素-Ⅰ免疫荧光组化法分析组织中半乳糖β-1,4-糖苷键的表达与定位,并与Ⅰ型前胶原α1共定位.结果 经糖链染色,与正常皮肤组织相比,瘢痕疙瘩组织在约30 000和40 000处糖蛋白的半乳糖β-1,4-糖苷键表达增高.偏振光显微镜显示,瘢痕疙瘩组织中含有大量Ⅰ型胶原纤维,约占(71.53±4.03)％,Ⅰ、Ⅲ型胶原比例为2.56±0.53,高于正常皮肤组织(P＜0.05).免疫荧光发现,半乳糖β-1,4-糖苷键均匀分布在瘢痕疙瘩组织成纤维细胞胞膜和胞质内,与正常皮肤组织相比其表达量明显增加,且与Ⅰ型前胶原α1存在共定位.结论 瘢痕疙瘩组织中存在着半乳糖β-1,4-糖苷键的表达变化,且主要定位于成纤维细胞,提示半乳糖β-1,4-糖苷键可能参与瘢痕疙瘩修复过程中纤维过度增生相关因子的修饰调控.
目的 探討半乳糖β-1,4-糖苷鍵在瘢痕疙瘩組織中的閤成、定位及糖蛋白半乳糖基化在瘢痕疙瘩形成機製中的作用.方法 Lectin印跡法觀察瘢痕疙瘩、增生性瘢痕和正常皮膚組織中糖蛋白的糖基化水平；飽和苦味痠-天狼猩紅偏振光法觀察組織學結構及膠原的形態與分佈.分析組織中Ⅰ、Ⅲ型膠原的比例；蓖痳凝集素-Ⅰ免疫熒光組化法分析組織中半乳糖β-1,4-糖苷鍵的錶達與定位,併與Ⅰ型前膠原α1共定位.結果 經糖鏈染色,與正常皮膚組織相比,瘢痕疙瘩組織在約30 000和40 000處糖蛋白的半乳糖β-1,4-糖苷鍵錶達增高.偏振光顯微鏡顯示,瘢痕疙瘩組織中含有大量Ⅰ型膠原纖維,約佔(71.53±4.03)％,Ⅰ、Ⅲ型膠原比例為2.56±0.53,高于正常皮膚組織(P＜0.05).免疫熒光髮現,半乳糖β-1,4-糖苷鍵均勻分佈在瘢痕疙瘩組織成纖維細胞胞膜和胞質內,與正常皮膚組織相比其錶達量明顯增加,且與Ⅰ型前膠原α1存在共定位.結論 瘢痕疙瘩組織中存在著半乳糖β-1,4-糖苷鍵的錶達變化,且主要定位于成纖維細胞,提示半乳糖β-1,4-糖苷鍵可能參與瘢痕疙瘩脩複過程中纖維過度增生相關因子的脩飾調控.
목적 탐토반유당β-1,4-당감건재반흔흘탑조직중적합성、정위급당단백반유당기화재반흔흘탑형성궤제중적작용.방법 Lectin인적법관찰반흔흘탑、증생성반흔화정상피부조직중당단백적당기화수평；포화고미산-천랑성홍편진광법관찰조직학결구급효원적형태여분포.분석조직중Ⅰ、Ⅲ형효원적비례；비마응집소-Ⅰ면역형광조화법분석조직중반유당β-1,4-당감건적표체여정위,병여Ⅰ형전효원α1공정위.결과 경당련염색,여정상피부조직상비,반흔흘탑조직재약30 000화40 000처당단백적반유당β-1,4-당감건표체증고.편진광현미경현시,반흔흘탑조직중함유대량Ⅰ형효원섬유,약점(71.53±4.03)％,Ⅰ、Ⅲ형효원비례위2.56±0.53,고우정상피부조직(P＜0.05).면역형광발현,반유당β-1,4-당감건균균분포재반흔흘탑조직성섬유세포포막화포질내,여정상피부조직상비기표체량명현증가,차여Ⅰ형전효원α1존재공정위.결론 반흔흘탑조직중존재착반유당β-1,4-당감건적표체변화,차주요정위우성섬유세포,제시반유당β-1,4-당감건가능삼여반흔흘탑수복과정중섬유과도증생상관인자적수식조공.
Objective To observe the expression and location of galactose β-1,4-glycosidic bonds (Gal β-1,4-GlcNAc) in keloid tissue,and to investigate the role of glycoprotein galactosylation in the formation of keloid.Methods This study included 10 keloid tissue specimens,7 hyperplastic scar tissue specimens,and 6 normal skin specimens.Lectin blot analysis was performed to measure the glycosylation level of glycoproteins,saturated picric acid-Sirius red staining followed by polarization microscopy to observe the type,expression and distribution of collagens in these specimens.The type Ⅰ/type Ⅲ collagen ratio was calculated.Immunofluorescence-based histochemistry was carried out by using Ricinus communis agglutinin I to analyze the expression and location of Gal β-1,4-GlcNAc in these skin samples,and double immunofluorescent staining to observe the colocalization of Gal β-1,4-GlcNAc and type Ⅰ procollagen α1.Results Compared with the normal skin tissue,the keloid tissue showed a significant increase in the expression of Gal β-1,4-GlcNAc in glycoproteins with a relative molecular mass of 30 000 to 40 000.Polarization microscopy revealed that there was a considerable expression of type Ⅰ collagen fibers,which amounted to (71.53 ± 4.03)％ in all the collagen fibers.The type Ⅰ/type Ⅲ collagen ratio was significantly higher in keloid tissue specimens than in normal tissue specimens (2.56 ± 0.53 vs.0.91 ± 0.11,P ＜0.05).Fluorescence microscopy showed that Gal β-1,4-GlcNAc was uniformly distributed in the membrane and cytoplasm of fibroblasts in keloid tissue,and the expression intensity of Gal β-1,4-GlcNAc in keloid tissue was notably stronger than that in normal skin tissue.There was a colocalization between Gal β-1,4-GlcNAc and type Ⅰ procollagen α1 in keloid tissue.Conclusions The expression of Gal β-1,4-GlcNAc,which is mainly observed in fibroblasts,is upregulated in keloid tissue,suggesting that Gal β-1,4-GlcNAc may be involved in the modulation of factors responsible for excessive fibre formation during the repair process of keloid.
