CAJ | 학술논문

目的探讨胶原三螺旋重复蛋白1(CTHRC1)、β联蛋白和Wnt5a在色素痣和黑素瘤不同发展阶段中的表达,探讨三者在黑素瘤发生、发展过程中的可能作用及相关性.方法免疫组化SP法检测23例色素痣、14例原位黑素瘤、21例侵袭性黑素瘤和18例转移性黑素瘤组织中CTHRC1、β联蛋白和Wnt5a蛋白的表达,结合临床病理特征进行分析.结果色素痣和黑素瘤组织中CTHRC1的阳性表达率分别为34.8％、62.3％,两者比较,差异有统计学意义(P＜0.05);β联蛋白的阳性表达率分别为21.7％、62.3％,两者比较,差异有统计学意义(P＜0.05)；Wnt5a的阳性表达率分别为34.8％、69.8％,两者比较,差异有统计学意义(P＜0.05).CTHRC1、β联蛋白的阳性表达与黑素瘤的浸润深度和淋巴结转移相关(P＜0.05),Wnt5a的阳性表达仅与淋巴结转移相关(P＜0.05).黑素瘤中CTHRC1与β联蛋白之间以及CTHRC1与Wnt5a蛋白之间的阳性表达存在明显正相关(分别为r=0.798,P＜0.01;r=0.623,P＜0.01).结论 CTHRC1、β联蛋白和Wnt5a在黑素瘤的发生中起一定的作用,CTHRC1可能作为Wnt信号通路的辅助蛋白促进黑素瘤的发生和发展.
목적 탐토효원삼라선중복단백1(CTHRC1)、β련단백화Wnt5a재색소지화흑소류불동발전계단중적표체,탐토삼자재흑소류발생、발전과정중적가능작용급상관성.방법 면역조화SP법검측23례색소지、14례원위흑소류、21례침습성흑소류화18례전이성흑소류조직중CTHRC1、β련단백화Wnt5a단백적표체,결합림상병리특정진행분석.결과 색소지화흑소류조직중CTHRC1적양성표체솔분별위34.8％、62.3％,량자비교,차이유통계학의의(P＜0.05);β련단백적양성표체솔분별위21.7％、62.3％,량자비교,차이유통계학의의(P＜0.05)；Wnt5a적양성표체솔분별위34.8％、69.8％,량자비교,차이유통계학의의(P＜0.05).CTHRC1、β련단백적양성표체여흑소류적침윤심도화림파결전이상관(P＜0.05),Wnt5a적양성표체부여림파결전이상관(P＜0.05).흑소류중CTHRC1여β련단백지간이급CTHRC1여Wnt5a단백지간적양성표체존재명현정상관(분별위r=0.798,P＜0.01;r=0.623,P＜0.01).결론 CTHRC1、β련단백화Wnt5a재흑소류적발생중기일정적작용,CTHRC1가능작위Wnt신호통로적보조단백촉진흑소류적발생화발전.
Objective To compare the protein expressions of collagen triple helix repeat containing-1 (CTHRC1),β-catenin and WntSa in lesions of nevus versus melanoma in different stages,and to assess their possible roles and relationship in the initiation and progression of melanoma.Methods Totally,76 tissue specimens were studied in this study,including 23 cases of nevus,14 cases of melanoma in situ,21 cases of invasive melanoma and 18 cases of metastatic melanoma.Immunohistochemical SP method was used to detect the protein expressions of CTHRC1,β-catenin and Wnt5a in these tissue specimens.The clinical and pathological implications of these protein expressions were assessed.Results The expression rate in nevus and melanoma lesions was 34.8％ and 62.3％ respectively for CTHRC1 (P ＜0.05),21.7％ and 62.3％ respectively for β-catenin (P ＜0.05),34.8％ and 69.8％ respectively for Wnt5a (P ＜0.05).The expressions of CTHRC1 and β-catenin were associated with the invasion depth and lymph node metastasis of melanoma (both P ＜0.05),and the expression of Wnt5a was only related to the lymph node metastasis of melanoma (P ＜0.05).There was a positive correlation between the protein expression of CTHRC1 and β-catenin as well as between that of CTHRC1 and Wnt5a (r =0.798,0.623,respectively,both P ＜0.01).Conclusions CTHRC1,β-catenin and Wnt5a appear to play a certain role in the development of melanoma,and CTHRC 1 may serve as a cofactor of the Wnt signaling pathway in promoting the initiation and progression of melanoma.