CAJ | 학술논문

目的探讨钠氢交换子调节因子1 (NHERF1)和β联蛋白在原发性乳房外Paget病组织中的表达及其意义.方法应用免疫组化方法,检测18例原位乳房外Paget病、22例侵袭性乳房外Paget病石蜡组织切片中NHERF1和β联蛋白的表达情况.结果 NHERF1在22例侵袭性乳房外Paget病中18例(81.82％)为高表达,18例原位乳房外Paget病中7例(38.89％)高表达,两组差异有统计学意义(x2=7.78,P＜0.01);β联蛋白在侵袭性乳房外Paget病中有0例胞膜高表达和18例(81.82％)胞质或核高表达,原位乳房外Paget病中分别为6例(33.33％)和8例(44.44％),两组差异均有统计学意义(x2值分别为8.63和6.08,P值分别＜ 0.01和＜0.05).原发性乳房外Paget病组织中NHERF1的表达与β联蛋白胞膜表达呈负相关(ρ=-0.488,P＜0.01),与β联蛋白胞质或核表达呈正相关(ρ=0.623,P＜ 0.01);β联蛋白胞膜表达与胞质或核表达呈负相关(ρ=-0.572,P＜ 0.01).结论乳房外Paget病组织中NHERF1和β联蛋白表达异常,并可能与原发性乳房外Paget病的发生发展及侵袭有关.
목적 탐토납경교환자조절인자1 (NHERF1)화β련단백재원발성유방외Paget병조직중적표체급기의의.방법 응용면역조화방법,검측18례원위유방외Paget병、22례침습성유방외Paget병석사조직절편중NHERF1화β련단백적표체정황.결과 NHERF1재22례침습성유방외Paget병중18례(81.82％)위고표체,18례원위유방외Paget병중7례(38.89％)고표체,량조차이유통계학의의(x2=7.78,P＜0.01);β련단백재침습성유방외Paget병중유0례포막고표체화18례(81.82％)포질혹핵고표체,원위유방외Paget병중분별위6례(33.33％)화8례(44.44％),량조차이균유통계학의의(x2치분별위8.63화6.08,P치분별＜ 0.01화＜0.05).원발성유방외Paget병조직중NHERF1적표체여β련단백포막표체정부상관(ρ=-0.488,P＜0.01),여β련단백포질혹핵표체정정상관(ρ=0.623,P＜ 0.01);β련단백포막표체여포질혹핵표체정부상관(ρ=-0.572,P＜ 0.01).결론 유방외Paget병조직중NHERF1화β련단백표체이상,병가능여원발성유방외Paget병적발생발전급침습유관.
Objective To investigate the expressions of Na+/H+ exchanger regulatory factor 1 (NHERF1) and β-catenin in extramammary Paget's disease tissue as well as their significance.Methods Immunohistochemistry was performed to detect the protein expressions of NHERF1 and β-catenin in paraffin-embeded tissue samples from 18 patients with in situ and 22 patients with invasive extramammary Paget's disease.Results There was a high expression of NHERF1 protein in 18 (81.82％) invasive and 7 (38.89％) in situ extramammary Paget's disease samples (x2 =7.78,P ＜ 0.01).Statistical differences were observed in the membrane expression rate and cytoplasmic or nuclear expression rate of β-catenin between invasive and in situ extramammary Paget's disease tissue samples (0 (0/22) vs.33.33％ (6/18),x2 =8.63,P ＜ 0.01; 81.82％ (18/22) vs.44.44％ (8/18),x2 =6.08,P ＜ 0.05).In extramammary Paget's disease in situ tissue samples,the expression of NHERF1 was negatively correlated with the cytomembrane expression of β-catenin (ρ =-0.488,P ＜ 0.01),but positively correlated with the cytoplasmic or nuclear expression of β-catenin (ρ =0.623,P ＜ 0.01),and there was a negative correlation between the cytomembrane and cytoplasmic or nuclear expression of β-catenin (ρ =-0.572,P ＜ 0.01).Conclusions There is an abnormal expression of NHERF1 and β-catenin in extramammary Paget's disease tissue,which may be associated with the initiation,progression,and invasion of primary extramammary Paget's disease.