CAJ | 학술논문

目的构建小鼠毛发周期的动物模型,筛选差异表达的微RNA.方法 30只雌性6～8周龄近交系C57BL/6小鼠,体重15 ～ 18 g.1％水合氯醛麻醉,松香/石蜡拔毛法拔除小鼠背部毛发.在拔毛后第0、8、20天,分别取3只小鼠,颈椎脱臼法处死,背部相同部位平行于脊椎取皮肤组织,按常规方法提取总RNA,进行微RNA微阵列芯片检测并行数据分析,发现其中有差异表达的微RNA.结果与休止期毛发皮肤组织相比,在生长期上调表达的有:miR-690、obselote-49、miR-1308,下调表达的有:miR-291a-5p、miR-212;在退行期上调表达的有:miR-690、obselote-49、MiR-31,下调表达的有:miR-127-3p、miR-212.结论初步筛选出小鼠毛发周期中皮肤组织存在的7个差异表达的微RNA,为今后其功能研究提供方向.
목적 구건소서모발주기적동물모형,사선차이표체적미RNA.방법 30지자성6～8주령근교계C57BL/6소서,체중15 ～ 18 g.1％수합록철마취,송향/석사발모법발제소서배부모발.재발모후제0、8、20천,분별취3지소서,경추탈구법처사,배부상동부위평행우척추취피부조직,안상규방법제취총RNA,진행미RNA미진렬심편검측병행수거분석,발현기중유차이표체적미RNA.결과 여휴지기모발피부조직상비,재생장기상조표체적유:miR-690、obselote-49、miR-1308,하조표체적유:miR-291a-5p、miR-212;재퇴행기상조표체적유:miR-690、obselote-49、MiR-31,하조표체적유:miR-127-3p、miR-212.결론 초보사선출소서모발주기중피부조직존재적7개차이표체적미RNA,위금후기공능연구제공방향.
Objective To screen differentially expressed microRNAs at different stages of hair cycle in a murine model.Methods This study included 30 inbred female C57BL/6 mice (age,6-8 weeks; body weight,15-18 grams).Hair growth cycle was induced in the back skin of C57BL/6 mice by application of wax/rosin followed by depilation under anesthesia witl 1％ chloral hydrate.Three mice were sacrificed by cervical dislocation on day 0,8 and 20 after the induction,and skin tissue was achieved from the same depilated areas parallel to the spine.Total RNAs were extracted from the murine skin and subjected to microarray analysis of microRNA expression.Results Compared with telogen skin,the murine anagen skin showed a higher expression of miR-690,obselote-49 and miR-1308,but a lower expression of miR-291a-5p and miR-212.The expressions of miR-690,obselote-49 and miR-31 were significantly up-regulated,while those of miR-127-3p and miR-212 were downregulated in the catagen skin in comparison with the telogen skin.Conclusion Seven microRNAs are identified in this study to be differentially expressed in murine skin between different stages of hair cycle,which may provide a direction for future research.