中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2013年
10期
707-710
,共4页
康尔恂%李杰%孙丽伟%韩春玉%闫丽萍%杨建
康爾恂%李傑%孫麗偉%韓春玉%閆麗萍%楊建
강이순%리걸%손려위%한춘옥%염려평%양건
荨麻疹%B淋巴细胞刺激因子%抗体,FcεRI%抗体,IgE%B淋巴细胞
蕁痳疹%B淋巴細胞刺激因子%抗體,FcεRI%抗體,IgE%B淋巴細胞
담마진%B림파세포자격인자%항체,FcεRI%항체,IgE%B림파세포
Urticaria%B cell activating factor%Antibodies,FcεRI%Antibodies,IgE%B-lymphocytes
目的 探讨B淋巴细胞刺激因子(BlyS)能否刺激慢性特发性荨麻疹(CIU)患者B淋巴细胞产生抗FcεRI抗体或抗IgE抗体.方法 设CIU患者组和健康对照组.ELISA法测定血清中BlyS、抗FcεRI抗体和抗IgE抗体水平,分离培养受试者外周血B淋巴细胞,在培养液中加入BlyS,检测培养液中抗FcεRI抗体和抗IgE抗体水平,分析BlyS与抗FcεRI抗体和抗IgE抗体产生的相关性.结果 CIU患者血清BlyS水平显著高于健康对照组(t=3.04,P＜ 0.01),抗FcεRI抗体和抗IgE抗体水平均显著高于健康对照组(t=3.51,P＜0.01;t=3.29,P＜ 0.01).CIU患者血清中抗FcεRI抗体和抗IgE抗体水平与BlyS水平呈正相关(r=0.93,P＜0.01;r=0.91,P＜ 0.01);CIU患者外周血B淋巴细胞培养液中加入有效浓度BlyS后,B淋巴细胞培养液中抗FcεRI抗体和抗IgE抗体水平均显著高于不加BlyS的空白对照(t=3.67,P＜ 0.01;t=3.56,P＜ 0.01),2种抗体在培养液中的水平与BlyS浓度呈正相关(r=0.96,P＜ 0.01;r=0.91,P＜ 0.01)；抗FcεRI抗体和抗IgE抗体在CIU患者血清与培养液中的检出符合率分别为94.76％和87.84％.结论 CIU患者血液中BlyS水平增高可刺激B淋巴细胞产生抗FcεRI抗体或抗IgE抗体,可能与CIU发病有关.
目的 探討B淋巴細胞刺激因子(BlyS)能否刺激慢性特髮性蕁痳疹(CIU)患者B淋巴細胞產生抗FcεRI抗體或抗IgE抗體.方法 設CIU患者組和健康對照組.ELISA法測定血清中BlyS、抗FcεRI抗體和抗IgE抗體水平,分離培養受試者外週血B淋巴細胞,在培養液中加入BlyS,檢測培養液中抗FcεRI抗體和抗IgE抗體水平,分析BlyS與抗FcεRI抗體和抗IgE抗體產生的相關性.結果 CIU患者血清BlyS水平顯著高于健康對照組(t=3.04,P＜ 0.01),抗FcεRI抗體和抗IgE抗體水平均顯著高于健康對照組(t=3.51,P＜0.01;t=3.29,P＜ 0.01).CIU患者血清中抗FcεRI抗體和抗IgE抗體水平與BlyS水平呈正相關(r=0.93,P＜0.01;r=0.91,P＜ 0.01);CIU患者外週血B淋巴細胞培養液中加入有效濃度BlyS後,B淋巴細胞培養液中抗FcεRI抗體和抗IgE抗體水平均顯著高于不加BlyS的空白對照(t=3.67,P＜ 0.01;t=3.56,P＜ 0.01),2種抗體在培養液中的水平與BlyS濃度呈正相關(r=0.96,P＜ 0.01;r=0.91,P＜ 0.01)；抗FcεRI抗體和抗IgE抗體在CIU患者血清與培養液中的檢齣符閤率分彆為94.76％和87.84％.結論 CIU患者血液中BlyS水平增高可刺激B淋巴細胞產生抗FcεRI抗體或抗IgE抗體,可能與CIU髮病有關.
목적 탐토B림파세포자격인자(BlyS)능부자격만성특발성담마진(CIU)환자B림파세포산생항FcεRI항체혹항IgE항체.방법 설CIU환자조화건강대조조.ELISA법측정혈청중BlyS、항FcεRI항체화항IgE항체수평,분리배양수시자외주혈B림파세포,재배양액중가입BlyS,검측배양액중항FcεRI항체화항IgE항체수평,분석BlyS여항FcεRI항체화항IgE항체산생적상관성.결과 CIU환자혈청BlyS수평현저고우건강대조조(t=3.04,P＜ 0.01),항FcεRI항체화항IgE항체수평균현저고우건강대조조(t=3.51,P＜0.01;t=3.29,P＜ 0.01).CIU환자혈청중항FcεRI항체화항IgE항체수평여BlyS수평정정상관(r=0.93,P＜0.01;r=0.91,P＜ 0.01);CIU환자외주혈B림파세포배양액중가입유효농도BlyS후,B림파세포배양액중항FcεRI항체화항IgE항체수평균현저고우불가BlyS적공백대조(t=3.67,P＜ 0.01;t=3.56,P＜ 0.01),2충항체재배양액중적수평여BlyS농도정정상관(r=0.96,P＜ 0.01;r=0.91,P＜ 0.01)；항FcεRI항체화항IgE항체재CIU환자혈청여배양액중적검출부합솔분별위94.76％화87.84％.결론 CIU환자혈액중BlyS수평증고가자격B림파세포산생항FcεRI항체혹항IgE항체,가능여CIU발병유관.
Objective To explore if B lymphocyte stimulator (BlyS) stimulates B lymphocytes from patients with chronic idiopathic urticaria (CIU) to produce anti-high affinity IgE receptor (FcεRI) or anti-IgE antibodies.Methods Totally,300 CIU patients and 300 health controls were enrolled in this study.Blood samples were obtained from these subjects.Peripheral blood B lymphocytes were isolated and cultured in vitro for 72 hours.Then,BlyS of various concentrations (2,4,8,16 ng/ml) was added to the culture medium of B lymphocytes followed by another 72-hour culture.Enzyme-linked immunosorbent assay was performed to determine the serum levels of BlyS,anti-FcεRI and anti-IgE antibodies,as well as the supernatant levels of anti-FcεRI and anti-IgE antibodies.The relationship between BlyS and anti-FcεRI and anti-IgE antibody production was assessed.SPSS software version 16.0 was used for statistical analysis.Chi-square test was performed to compare the positivity rate of antibodies,and analysis of variance and least significance difference-t test to assess numerical data.Results The CIU patients showed higher levels of serum BlyS (t =3.04,P ＜ 0.01),anti-FcεRI antibodies (t =3.51,P ＜ 0.01),and anti-IgE antibodies (t =3.29,P ＜ 0.01) compared with the health controls.The serum level of BlyS was positively correlated with that of anti-FcεRI antibodies (r =0.93,P ＜ 0.01) and anti-IgE antibodies (r =0.91,P ＜ 0.01).The levels of anti-FcεRI antibodies and anti-IgE antibodies were significantly increased in the culture supematant of patient-derived B lymphocytes treated with BlyS compared with those remaining untreated (t =3.67,3.56,respectively,both P ＜ 0.01),and the concentration of BlyS was positively correlated with the levels of both anti-FcεRI antibodies and anti-IgE antibodies (r =0.96,0.91,respectively,both P ＜ 0.01).The coincidence rate between the serum and supernatant was 94.76％ and 87.84％ in the detection of anti-FcεRI antibodies and anti-IgE antibodies respectively.Conclusions BlyS level is upregulated in the serum of patients with CIU,which may play an important role in the pathogenesis of CIU by stimulating B lymphocytes to produce anti-FcεRI antibodies or anti-IgE antibodies.
