中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
1期
19-21
,共3页
张宝祥%林茂%段志武%张佃财
張寶祥%林茂%段誌武%張佃財
장보상%림무%단지무%장전재
皮炎,特应性%CD8阳性T淋巴细胞%皮肤淋巴细胞相关抗原%颗粒酶类%受体,趋化因子
皮炎,特應性%CD8暘性T淋巴細胞%皮膚淋巴細胞相關抗原%顆粒酶類%受體,趨化因子
피염,특응성%CD8양성T림파세포%피부림파세포상관항원%과립매류%수체,추화인자
Dermatitis,atopic%CD8-positive T-lymphocytes%Cutaneous lymphocyte-associated antigen%Grazymes%Receptors,chemokine
目的 探讨特应性皮炎(AD)患者外周血CD8+T细胞皮肤归巢及杀伤功能相关蛋白的表达.方法 15例AD患者和14例健康人,用流式细胞仪,检测外周血CD8+T细胞、表达皮肤淋巴细胞相关抗原的CD8+T细胞(CLA+CD8+T细胞)的比例.结果 CD8+T细胞比例在AD组和对照组之间差异无统计学意义(P＞ 0.05);CD8+T细胞穿孔素、颗粒酶B和FasL的表达在两组间差异亦无统计学意义(均P＞0.05).CLA+℃D8+T细胞比例在AD组(3.80％±1.46％)高于健康对照组(2.18％±0.85％)(t=3.636,P＜ 0.01),AD组CLA+CD8+T细胞比例与SCORAD(SCORing of Atopic Dermatitis)评分呈正相关(r=0.565,P＜0.05);CCR4在CD8+T细胞表达在AD组(13.86％±4.42％)高于健康对照组(9.50％±2.14％)(t=3.738,P＜ 0.01),而CCR10和CXCR6的表达在两组间差异均无统计学意义(P＞0.05).CLA+CD8+T细胞穿孔素的表达在AD组(74.27％±15.94％)高于健康对照组(57.20％±14.64％)(t=2.998,P＜ 0.01),颗粒酶B的表达在AD组(70.90％±13.85％)也高于健康对照组(56.41％±11.00％)(t=3.104,P＜ 0.01),而FasL的表达在两组间差异无统计学意义(P＞0.05).CLA+CD8+T细胞CCR4、CCR10和CXCR6的表达在AD组与对照组间差异无统计学意义(均P＞0.05).结论 AD患者外周血CLA+CD8+T细胞数量增加,杀伤功能相关蛋白穿孔素、颗粒酶B的表达增强,可能参与了AD的发病.
目的 探討特應性皮炎(AD)患者外週血CD8+T細胞皮膚歸巢及殺傷功能相關蛋白的錶達.方法 15例AD患者和14例健康人,用流式細胞儀,檢測外週血CD8+T細胞、錶達皮膚淋巴細胞相關抗原的CD8+T細胞(CLA+CD8+T細胞)的比例.結果 CD8+T細胞比例在AD組和對照組之間差異無統計學意義(P＞ 0.05);CD8+T細胞穿孔素、顆粒酶B和FasL的錶達在兩組間差異亦無統計學意義(均P＞0.05).CLA+℃D8+T細胞比例在AD組(3.80％±1.46％)高于健康對照組(2.18％±0.85％)(t=3.636,P＜ 0.01),AD組CLA+CD8+T細胞比例與SCORAD(SCORing of Atopic Dermatitis)評分呈正相關(r=0.565,P＜0.05);CCR4在CD8+T細胞錶達在AD組(13.86％±4.42％)高于健康對照組(9.50％±2.14％)(t=3.738,P＜ 0.01),而CCR10和CXCR6的錶達在兩組間差異均無統計學意義(P＞0.05).CLA+CD8+T細胞穿孔素的錶達在AD組(74.27％±15.94％)高于健康對照組(57.20％±14.64％)(t=2.998,P＜ 0.01),顆粒酶B的錶達在AD組(70.90％±13.85％)也高于健康對照組(56.41％±11.00％)(t=3.104,P＜ 0.01),而FasL的錶達在兩組間差異無統計學意義(P＞0.05).CLA+CD8+T細胞CCR4、CCR10和CXCR6的錶達在AD組與對照組間差異無統計學意義(均P＞0.05).結論 AD患者外週血CLA+CD8+T細胞數量增加,殺傷功能相關蛋白穿孔素、顆粒酶B的錶達增彊,可能參與瞭AD的髮病.
목적 탐토특응성피염(AD)환자외주혈CD8+T세포피부귀소급살상공능상관단백적표체.방법 15례AD환자화14례건강인,용류식세포의,검측외주혈CD8+T세포、표체피부림파세포상관항원적CD8+T세포(CLA+CD8+T세포)적비례.결과 CD8+T세포비례재AD조화대조조지간차이무통계학의의(P＞ 0.05);CD8+T세포천공소、과립매B화FasL적표체재량조간차이역무통계학의의(균P＞0.05).CLA+℃D8+T세포비례재AD조(3.80％±1.46％)고우건강대조조(2.18％±0.85％)(t=3.636,P＜ 0.01),AD조CLA+CD8+T세포비례여SCORAD(SCORing of Atopic Dermatitis)평분정정상관(r=0.565,P＜0.05);CCR4재CD8+T세포표체재AD조(13.86％±4.42％)고우건강대조조(9.50％±2.14％)(t=3.738,P＜ 0.01),이CCR10화CXCR6적표체재량조간차이균무통계학의의(P＞0.05).CLA+CD8+T세포천공소적표체재AD조(74.27％±15.94％)고우건강대조조(57.20％±14.64％)(t=2.998,P＜ 0.01),과립매B적표체재AD조(70.90％±13.85％)야고우건강대조조(56.41％±11.00％)(t=3.104,P＜ 0.01),이FasL적표체재량조간차이무통계학의의(P＞0.05).CLA+CD8+T세포CCR4、CCR10화CXCR6적표체재AD조여대조조간차이무통계학의의(균P＞0.05).결론 AD환자외주혈CLA+CD8+T세포수량증가,살상공능상관단백천공소、과립매B적표체증강,가능삼여료AD적발병.
Objective To quantify the percentage of CD8+ T cells and their expressions of cytotoxic molecules and homing-related chemokine receptors in peripheral blood from patients with atopic dermatitis (AD).Methods Peripheral blood was obtained from 15 patients with AD and 14 healthy controls.Flow cytometric analysis was performed to determine the percentages of CD8+ T cells and CD8+CLA+ T cells in the peripheral blood samples,as well as the expression levels of cytotoxic molecules and homing-related chemokine receptors on these cells.Differences in these parameters were analyzed using t test,and relationship between these parameters was evaluated using Pearson correlation coefficient.Results No significant difference was observed between the patients with AD and healthy controls in the percentage of CD8+ T cells,the expressions of perforin,granzyme B,CCR10,CCR6 or FasL on CD8+ T cells,or the expressions of CCR4,CCR10,CXCR6 or FasL on CLA+CD8+ T cells (all P ＞ 0.05).A significant increase was noted in the percentage of CLA+CD8+ T cells (3.80％ ± 1.46％ vs.2.18％ ± 0.85％,t =3.636,P ＜ 0.01) and expression rates of CCR4 on CD8+ T cells (13.86％ ± 4.42％ vs.9.50％ ± 2.14％,t =3.738,P ＜ 0.01) as well as perforin and granzyme B on CLA+CD8+ T cells (74.27％ ± 15.94％ vs.57.20％ ± 14.64％,t =2.998,P ＜ 0.01; 70.90％ ± 13.85％ vs.56.41％ ± 11.00％,t =3.104,P ＜ 0.01) in the patients with AD compared with the healthy controls.Conclusions The proportion of CLA+CD8+ T cells is increased with enhanced expressions of cytotoxic molecules such as perforin and granzyme B in peripheral blood of patients with AD,which may contribute to the pathogenesis of AD.
