CAJ | 학술논문

目的探讨CD4+CD25+T细胞与斑秃发病之间的关系.方法收集了3组研究对象,其中健康对照组25例、稳定期斑秃患者25例、进展期斑秃患者23例.抽取所有对象外周血,提取CD4+CD25+T细胞,培养4d,收集培养上清液,ELISA法检测上清液IL-10和TGF-β1水平.结果进展期斑秃患者外周血CD4+CD25+T细胞培养的IL-10和TGF-β1分别为(31.68±6.78) pg/ml和(32.29±6.80) pg/ml,明显低于健康对照组(57.34±14.15) pg/ml、(57.43±15.16) pg/ml和稳定期斑秃患者(52.56±13.02) pg/ml和(61.75±14.10) pg/ml(P＜ 0.05),而健康对照组和稳定期斑秃患者之间差异无统计学意义(P＞0.05).结论 CD4+CD25+T细胞分泌IL-10和TGF-β1功能降低,在斑秃发病中有一定意义.
목적 탐토CD4+CD25+T세포여반독발병지간적관계.방법 수집료3조연구대상,기중건강대조조25례、은정기반독환자25례、진전기반독환자23례.추취소유대상외주혈,제취CD4+CD25+T세포,배양4d,수집배양상청액,ELISA법검측상청액IL-10화TGF-β1수평.결과 진전기반독환자외주혈CD4+CD25+T세포배양적IL-10화TGF-β1분별위(31.68±6.78) pg/ml화(32.29±6.80) pg/ml,명현저우건강대조조(57.34±14.15) pg/ml、(57.43±15.16) pg/ml화은정기반독환자(52.56±13.02) pg/ml화(61.75±14.10) pg/ml(P＜ 0.05),이건강대조조화은정기반독환자지간차이무통계학의의(P＞0.05).결론 CD4+CD25+T세포분비IL-10화TGF-β1공능강저,재반독발병중유일정의의.
Objective To evaluate the potential association of CD4+CD25+ T cells with alopecia areata.Methods Totally,this study enrolled 23 patients with progressive alopecia areata,25 patients with stable alopecia areata,and 25 healthy controls.Peripheral blood was isolated from these subjects followed by isolation of CD4+ CD25+ regulatory T cells,which were then cuhured with the presence of anti-CD3 and-CD28 monoclonal antibodies for four days.Subsequently,enzyme-linked immunosorbent assay was performed to measure the levels of interleukin (IL)-10 and transforming growth factor (TGF)-β1 in the culture supematant of these T cells.Results The levels of IL-10 and TGF-β1 were (31.68 ± 6.78) pg/ml and (32.29 ± 6.8) pg/ml respectively in the culture supernatant of CD4+CD25+ regulatory T cells from patients with progressive alopecia areata,significantly lower than those from the healthy controls ((57.34 ± 14.15) pg/ml and (57.43 ± 15.16) pg/ml,both P ＜ 0.05) and patients with stable alopecia areata ((52.56 ± 13.02) pg/ml and (61.75 ± 14.10) pg/ml,both P ＜ 0.05).However,no significant difference was observed in the supernatant levels of IL-10 or TGF-β1 between the healthy controls and patients with stable alopecia areata.Conclusions The secretion of IL-10 and TGF-β1 by CD4+CD25+ T cells is decreased in patients with progressive alopecia areata,which may contribute to the pathogenesis of alopecia areata.