CAJ | 학술논문

目的探讨窄谱中波紫外线(NB-UVB)对角质形成细胞角蛋白17(K17)表达的影响.方法不同剂量NB-UVB照射角质形成细胞后,培养6、12、24h,分别用CCK8法检测细胞增殖情况,实时PCR、Western印迹检测K17 mRNA和蛋白表达以及Erk 1/2、磷酸化Erk 1/2的表达水平.结果低剂量(50、100 mJ/cm2)NB-UVB照射可刺激角质形成细胞增殖和K17表达,而较高剂量(200、400 mJ/cm2)NB-UVB照射则抑制细胞增殖和K17表达,其差异有统计学意义(P＜0.05或0.01).通过对NB-UVB照射后相关信号通路进行筛查,发现100 mJ/em2 NB-UVB照射可以上调角质形成细胞Erk1/2的磷酸化水平,而400 mJ/cm2 NB-UVB照射后Erk1/2的磷酸化水平明显下降,阻断这一信号通路可以抑制低剂量NB-UVB对K17的上调.结论 NB-UVB对K17表达的影响受Erk1/2通路的调控.
목적 탐토착보중파자외선(NB-UVB)대각질형성세포각단백17(K17)표체적영향.방법 불동제량NB-UVB조사각질형성세포후,배양6、12、24h,분별용CCK8법검측세포증식정황,실시PCR、Western인적검측K17 mRNA화단백표체이급Erk 1/2、린산화Erk 1/2적표체수평.결과 저제량(50、100 mJ/cm2)NB-UVB조사가자격각질형성세포증식화K17표체,이교고제량(200、400 mJ/cm2)NB-UVB조사칙억제세포증식화K17표체,기차이유통계학의의(P＜0.05혹0.01).통과대NB-UVB조사후상관신호통로진행사사,발현100 mJ/em2 NB-UVB조사가이상조각질형성세포Erk1/2적린산화수평,이400 mJ/cm2 NB-UVB조사후Erk1/2적린산화수평명현하강,조단저일신호통로가이억제저제량NB-UVB대K17적상조.결론 NB-UVB대K17표체적영향수Erk1/2통로적조공.
Objective To investigate the effects of narrow-band ultraviolet B (NB-UVB) on the expression of keratin 17 (K17) in the human keratinocyte cell line HaCaT.Methods Cultured HaCaT cells were irradiated with different doses (0,50,100,200,400 mJ/cm2) of NB-UVB followed by additional culture for 6,12 and 24 hours respectively.To explore the mechanisms underlying the effects of NB-UVB on K17 expression,some HaCaT cells were pretreated with PD98059 (an inhibitor of mitogen-activated protein kinase kinase) followed by UVB radiation and additional culture as described above.Cell counting kit-8 (CCK 8) was used to evaluate the proliferation of,real time PCR to measure the mRNA expressions of K17 in,and Western blot to determine the protein expression of K17,Erk1/2 and phosphorylated Erk1/2 in,HaCaT ceils.Results Both the proliferation of and K17 expression in HaCaT cells were promoted by NB-UVB radiation at low doses (50,100 mJ/cm2),but inhibited by NB-UVB radiation at high doses (200,400 mJ/cm2).Significant differences were observed for the proliferative activity between HaCaT cells irradiated with NB-UVB at 100 or 400 mJ/cm2 and unirradiated HaCaT ceils at 12 and 24 hours (P ＜ 0.01 or 0.05).The phosphorylation of Erk1/2 was upregulated by NB-UVB radiation at 100 mJ/cm2,but downregulated by that at 400 mJ/cm2,and the upregulation induced by low dose NB-UVB could be suppressed by blocking the Erk 1/2 pathway.Conclusion The effects of NB-UVB radiation on K17 expression may be modulated by the Erk 1/2 pathway.