CAJ | 학술논문

目的检测神经生长因子(NGF)及其受体TrkA、p75NTR在扁平苔藓皮损中的表达.方法应用免疫组化ABC法检测32例扁平苔藓皮损和12例健康人皮肤石蜡标本NGF及其受体TrkA、p75NTR表达状况.结果 NGF及TrkA在32例扁平苔藓皮损表皮角质形成细胞中均有不同程度的表达(++～+++),表达部位为细胞质,高于健康人皮肤NGF(-～+)及TrkA(-～+)的表达,两组间差异均有统计学意义(P＜0.01);而p75NTR的表达两组差异无统计学意义.扁平苔藓皮损中NGF与TrkA表达呈正相关(R2=0.535,P＜ 0.01).NGF及其受体TrkA、p75NTR在扁平苔藓不同发病年龄、部位以及不同性别患者角质形成细胞中的表达差异均无统计学意义.结论 NGF通过其高亲和受体TrkA在扁平苔藓的发病中可能起着一定的作用.
목적 검측신경생장인자(NGF)급기수체TrkA、p75NTR재편평태선피손중적표체.방법 응용면역조화ABC법검측32례편평태선피손화12례건강인피부석사표본NGF급기수체TrkA、p75NTR표체상황.결과 NGF급TrkA재32례편평태선피손표피각질형성세포중균유불동정도적표체(++～+++),표체부위위세포질,고우건강인피부NGF(-～+)급TrkA(-～+)적표체,량조간차이균유통계학의의(P＜0.01);이p75NTR적표체량조차이무통계학의의.편평태선피손중NGF여TrkA표체정정상관(R2=0.535,P＜ 0.01).NGF급기수체TrkA、p75NTR재편평태선불동발병년령、부위이급불동성별환자각질형성세포중적표체차이균무통계학의의.결론 NGF통과기고친화수체TrkA재편평태선적발병중가능기착일정적작용.
Objective To detect the expressions of nerve growth factor (NGF) and its receptors tyrosine kinase A (TrkA) as well as p75 neurotrophin receptor (p75NTR) in the lesions of lichen planus.Methods Biopsy specimens were collected from the lesions of 32 patients with lichen planus and normal skin of 12 healthy human controls and subjected to paraffin embedding.Immunohistochemical avidin-biotin complex (ABC) method was used to detect the expressions of NGF,TrkA and p75NTR.Results NGF and TrkA,which were located in the cytoplasm of keratinocytes,were strongly or moderately expressed in the lesional skin specimens,but absent or weakly expressed in the normal skin specimens (both P ＜ 0.01).No significant differences were observed in the expression of p75NTR between the lesional and normal skin specimens,or in the expressions of NGF,TrkA or p75NTR among specimens from patients in different age groups,patients of different gender or lesions at different sites (all P ＞ 0.05).There was a positive correlation between the expression of NGF and TrkA in the lesions of lichen planus (R2 =0.535,P ＜ 0.01).Conclusion NGF may play a certain role in the development of lichen planus via its highaffinity receptor TrkA.