CAJ | 학술논문

目的探讨慢性荨麻疹患者外周血中白介素(IL)-25、IL-33的表达及意义.方法慢性荨麻疹患者93例,健康人对照30例.慢性荨麻疹组口服氯雷他定治疗4周.采用酶联免疫吸附试验(ELISA)检测外周血IL-25、IL-33水平,分析慢性荨麻疹患者治疗前IL-25、IL-33水平与病情严重程度的相关性.结果慢性荨麻疹患者治疗前IL-25、IL-33水平分别为(139.86±28.48)、(91.95±21.88) ng/L,健康对照组分别为(114.41±34.00)、(79.80±30.72) ng/L,慢性荨麻疹患者IL-25、IL-33水平较健康对照组显著升高(P＜0.01和＜0.05),且与患者病情呈正相关(r分别为0.38和0.42,均P＜0.01).慢性荨麻疹患者口服氯雷他定治疗4周后,有效率为81.72％,患者IL-25表达水平为(116.48±21.94) ng/L,较治疗前明显降低(P＜0.01),与健康对照组相比差异无统计学意义(P＞ 0.05);IL-33表达水平为(90.88±20.62) ng/L,较治疗前差异无统计学意义(P＞0.05).结论 IL-25、IL-33在慢性荨麻疹患者外周血中表达水平升高,并与病情严重程度呈正相关.
목적 탐토만성담마진환자외주혈중백개소(IL)-25、IL-33적표체급의의.방법 만성담마진환자93례,건강인대조30례.만성담마진조구복록뢰타정치료4주.채용매련면역흡부시험(ELISA)검측외주혈IL-25、IL-33수평,분석만성담마진환자치료전IL-25、IL-33수평여병정엄중정도적상관성.결과 만성담마진환자치료전IL-25、IL-33수평분별위(139.86±28.48)、(91.95±21.88) ng/L,건강대조조분별위(114.41±34.00)、(79.80±30.72) ng/L,만성담마진환자IL-25、IL-33수평교건강대조조현저승고(P＜0.01화＜0.05),차여환자병정정정상관(r분별위0.38화0.42,균P＜0.01).만성담마진환자구복록뢰타정치료4주후,유효솔위81.72％,환자IL-25표체수평위(116.48±21.94) ng/L,교치료전명현강저(P＜0.01),여건강대조조상비차이무통계학의의(P＞ 0.05);IL-33표체수평위(90.88±20.62) ng/L,교치료전차이무통계학의의(P＞0.05).결론 IL-25、IL-33재만성담마진환자외주혈중표체수평승고,병여병정엄중정도정정상관.
Objective To detect the expression levels of interleukin (IL)-25 and IL-33 in peripheral blood of patients with chronic urticaria.Methods Ninety-three patients with chronic urticaria were included in this study along with 30 healthy individuals.All the patients were treated with loratadine for four weeks.Blood samples were collected from the healthy controls and patients before and after the four-week treatment.Enzyme-linked immunosorbent assay (ELISA) was performed to detect the serum levels of IL-25 and IL-33.The relationship between the expression levels of the two cytokines and urticaria severity was analyzed.Results The serum levels of IL-25 and IL-33 in the patients before treatment were significantly higher than those in the healthy controls (IL-25,139.86 ± 28.48 vs.114.41 ± 34.00 ng/L,P ＜ 0.01; IL-33,91.95 ± 21.88 vs.79.80 ± 30.72 ng/L,P ＜ 0.05),and positively correlated with the severity of urticaria (r =0.38,0.42,respectively,both P ＜ 0.01).After four weeks of treatment,clinical improvement was observed in 81.72％ of these patients with a significant decrease in the serum IL-25 level (116.48 ± 21.94 vs.139.86 ± 28.48 ng/L,P ＜ 0.01),but no obvious changes in the serum IL-33 level (90.88 ± 20.62 vs.91.95 ± 21.88 ng/L,P ＞ 0.05) compared with those before treatment.Conclusions The expressions of IL-25 and IL-33 are elevated in peripheral blood of patients with chronic urticaria,and positively correlated with the severity of urticaria.