CAJ | 학술논문

目的探讨相同条件下,解脲脲原体血清1型(Up1)和8型(Uu8)在BALB/c小鼠生殖道感染模型中的致病性.方法 48只BALB/c小鼠随机分为空白对照组、雌激素组、Up1感染组、Uu8感染组,每组12只.分别接种无菌液体培养基、Up1、Uu8标准菌液.接种后第3、7、14、21天,每组随机取3只小鼠,采集阴道灌洗液后处死,取阴道、子宫标本固定后,HE染色镜检.阴道灌洗液用于培养和肿瘤坏死因子(TNF)-α检测.结果接种后空白组和雌激素组均无解脲脲原体生长,TNF-α为(4.17±0.85) pg/ml.两个感染组各时间点小鼠阴道冲洗液经培养均为阳性,随着时间延长,测定颜色改变单位逐渐下降.TNF-α在感染后第14天达峰值,分别为(14.93±1.11) pg/ml和(27.04±24.26) pg/ml.组织病理结果显示,Up1和Uu8感染后均可导致急慢性炎症反应,以子宫病变为主,Up1可导致宫腔粘连.结论 BALB/c小鼠下生殖道感染Up1和Uu8后,主要导致宫颈炎,两组致病性未见明显差异.Up1可能与宫腔粘连有关.
목적 탐토상동조건하,해뇨뇨원체혈청1형(Up1)화8형(Uu8)재BALB/c소서생식도감염모형중적치병성.방법 48지BALB/c소서수궤분위공백대조조、자격소조、Up1감염조、Uu8감염조,매조12지.분별접충무균액체배양기、Up1、Uu8표준균액.접충후제3、7、14、21천,매조수궤취3지소서,채집음도관세액후처사,취음도、자궁표본고정후,HE염색경검.음도관세액용우배양화종류배사인자(TNF)-α검측.결과 접충후공백조화자격소조균무해뇨뇨원체생장,TNF-α위(4.17±0.85) pg/ml.량개감염조각시간점소서음도충세액경배양균위양성,수착시간연장,측정안색개변단위축점하강.TNF-α재감염후제14천체봉치,분별위(14.93±1.11) pg/ml화(27.04±24.26) pg/ml.조직병리결과현시,Up1화Uu8감염후균가도치급만성염증반응,이자궁병변위주,Up1가도치궁강점련.결론 BALB/c소서하생식도감염Up1화Uu8후,주요도치궁경염,량조치병성미견명현차이.Up1가능여궁강점련유관.
Objective To compare the pathogenicity between Ureaplasma urealyticum serotype 1 (Up1)and 8 (Uu8) in the genital tract of BALB/c mice.Methods A total of 48 BALB/c mice were randomly and equally divided into four groups:blank control group receiving no treatment,estradiol group pretreated with intramuscular injection of estradiol followed by intravaginal inoculation with sterial liquid culture media,Up1 and Uu8 groups pretreated with intramuscular injection of estradiol followed by intravaginal inoculation with suspensions of Up1 and Uu8 respectively.Three mice were randomly selected from each group to be sacrificed after the collection of vaginal lavage fluid on day 3,7,14 and 21 after the inoculation.Vaginal and uterine tissue specimens were obtained from these sacrificed mice and underwent hematoxylin and eosin (HE) staining.Vaginal lavage fluid samples were subjected to culture of Uu and measurement of tumor necrosis factor-α (TNF-α).Results No evidences were observed for Uu growth in either the blank control group or estradiol group at any of the time points after the inoculation,with the average level of TNF-α in vaginal lavage fluid being (4.17 ± 0.85) pg/ml at these time points in both groups.Uu grew in all the vaginal lavage fluid samples from the Up1 and Uu8 groups at the four time points,with the color change unit (CCU) value decreasing with time.The level of TNF-α in vaginal lavage fluid peaked on day 14 after the inoculation in the Up 1 ((14.93 ± 1.11) pg/ml) and Uu8 ((27.04 ± 24.26) pg/ml) groups.Both Up1 and Uu8 infection caused acute and chronic inflammatory responses in the mice,which were mainly located in the uterus,and Up1 might cause intrauterine adhesion.Conclusions At the same inoculation concentration,no significant difference is found in the pathogenicity between Up1 and Uu8,both of which appear to mainly cause cervicitis.Upl might be partially responsible for intrauterine adhesion in mice.