CAJ | 학술논문

目的了解北京市男男性行为(MSM)人群HIV及梅毒感染状况,探讨两者之间的相关性.方法对北京市艾滋病哨点监测MSM人群600例进行HIV及梅毒实验室检测.用ELISA方法对所有研究对象样本进行HIV初筛,初筛阳性者用免疫印迹方法进行确认;梅毒检测采用快速血浆反应素环状卡片试验(RPR)与梅毒螺旋体明胶凝集试验(TPPA)两种方法同时进行,TPPA阳性者即视为梅毒感染者(包括既往感染与现症感染);符合条件的HIV阳性血清还接受HIV-1 BED捕获酶联法(BED方法)检测并计算HIV在此人群中的发病率;对MSM人群HIV及梅毒感染状况进行统计学相关性分析.结果 MSM人群600例,HIV阳性49例,阳性率8.17％;HIV新近感染14例,发病率为4.68％.HIV感染者表现出外地户籍比重大、学历层次相对较高的特点.梅毒RPR与TPPA均阳性51例(8.5％),感染率(含既往感染)为18.5％(111/600).结论北京市哨点监测MSM人群HIV感染率及发病率、梅毒感染率均维持在较高水平;HIV感染与梅毒感染有显著相关性.
목적 료해북경시남남성행위(MSM)인군HIV급매독감염상황,탐토량자지간적상관성.방법 대북경시애자병초점감측MSM인군600례진행HIV급매독실험실검측.용ELISA방법대소유연구대상양본진행HIV초사,초사양성자용면역인적방법진행학인;매독검측채용쾌속혈장반응소배상잡편시험(RPR)여매독라선체명효응집시험(TPPA)량충방법동시진행,TPPA양성자즉시위매독감염자(포괄기왕감염여현증감염);부합조건적HIV양성혈청환접수HIV-1 BED포획매련법(BED방법)검측병계산HIV재차인군중적발병솔;대MSM인군HIV급매독감염상황진행통계학상관성분석.결과 MSM인군600례,HIV양성49례,양성솔8.17％;HIV신근감염14례,발병솔위4.68％.HIV감염자표현출외지호적비중대、학력층차상대교고적특점.매독RPR여TPPA균양성51례(8.5％),감염솔(함기왕감염)위18.5％(111/600).결론 북경시초점감측MSM인군HIV감염솔급발병솔、매독감염솔균유지재교고수평;HIV감염여매독감염유현저상관성.
Objective To determine the prevalence of and correlations between HIV infection and syphilis among men who have sex with men (MSM) in Beijing.Methods Totally,600 MSM collected from sentinel surveillance sites for AIDS in Beijing were recruited in this study,and underwent screening tests for HIV infection and syphilis.Enzyme-linked immunosorbent assay (ELISA) was used for the primary screening for HIV infection,and Western blot for its confirmation.Both nontreponemal test (rapid plasma regain (RPR) assay) and treponemal test (Treponema pallidum particle agglutination assay,TPPA) were performed to detect syphilis,and only those with a reactive TPPA result were defined as having syphilis (including past infection).Specimens confirmed as HIV-1 positive were further tested with BED-capture enzyme immunoassay (CEIA) to find recent HIV-1 infection,and the incidence of HIV infection was then calculated.The correlation between syphilis and HIV infection was statistically analyzed.Results Of the 600 MSM,49 (8.17％) had HIV infection,including 14 cases of recent infection,with the incidence rate of HIV infection being 4.68％; 51 were positive for both RPR and TPPA tests with the infection rate (including past infection) being 18.5％ (111/600).People without a Beijing household registration and those with high education levels accounted for a large proportion of HIV-infected MSM.Conclusions The prevalence and incidence of HIV infection as well as prevalence of syphilis maintain at a relatively high level in MSM in Beijing.HIV infection is markedly correlated with syphilis in MSM population.