瘢痕,肥大性%维生素A酸类%疾病模型,动物
瘢痕,肥大性%維生素A痠類%疾病模型,動物
반흔,비대성%유생소A산류%질병모형,동물
Cicatrix,hypertrophic%Retinoids%Disease models,animal
目的 研究维A酸涂膜剂对兔耳早期增生性瘢痕的影响,探讨其防治瘢痕的可行性.方法 选取新西兰白兔24只,建立兔耳增生性瘢痕模型后,随机分为4组,每组6只.A组:对照组,B组:涂膜剂组,C组:0.05％维A酸涂膜剂组,D组:0.1％维A酸涂膜剂组,连续用药6周,期间观察记录瘢痕大小、厚度、颜色、硬度,6周后分别切取兔耳瘢痕组织,HE染色,胶原染色(VG法),行病理学观察、检测及分析.结果 A组瘢痕颜色深、厚而硬,并明显高于皮肤,表面凹凸不平;B组、C组、D组瘢痕颜色浅,质地软,厚度薄,皮下结节小,其中C、D组与周围正常皮肤接近,D组瘢痕表面有脱皮现象.HE、VG染色中,A组胶原排列紊乱,有旋涡状结构;C组和D组单位面积内成纤维细胞、微血管数量、胶原沉积量较A组和B组少,且胶原排列整齐,与瘢痕长轴平行.瘢痕增生指数(HI):A组3.17±0.26,B组2.46±0.19,C组1.91±0.21,D组1.90±0.23;成纤维细胞密度(NA):A组5836.70±527.03,B组4128.73±387.66,C组3207.59±439.17,D组3200.28±421.48;胶原纤维的面密度(AA):A组45.38±5.83,B组36.57±6.84,C组28.09±3.82,D组28.07±3.47.A组与B、C、D组比较,HI、NA、AA值差异均有统计学意义(P＜0.01);B组与C、D组比较,HI、NA、AA值差异均有统计学意义(P＜0.01);C组与D组比较,HI、NA、AA值差异无统计学意义(P＞0.05).结论 维A酸涂膜剂可以抑制兔耳早期瘢痕增生,可为防治瘢痕提供一种新的外用方法.
目的 研究維A痠塗膜劑對兔耳早期增生性瘢痕的影響,探討其防治瘢痕的可行性.方法 選取新西蘭白兔24隻,建立兔耳增生性瘢痕模型後,隨機分為4組,每組6隻.A組:對照組,B組:塗膜劑組,C組:0.05％維A痠塗膜劑組,D組:0.1％維A痠塗膜劑組,連續用藥6週,期間觀察記錄瘢痕大小、厚度、顏色、硬度,6週後分彆切取兔耳瘢痕組織,HE染色,膠原染色(VG法),行病理學觀察、檢測及分析.結果 A組瘢痕顏色深、厚而硬,併明顯高于皮膚,錶麵凹凸不平;B組、C組、D組瘢痕顏色淺,質地軟,厚度薄,皮下結節小,其中C、D組與週圍正常皮膚接近,D組瘢痕錶麵有脫皮現象.HE、VG染色中,A組膠原排列紊亂,有鏇渦狀結構;C組和D組單位麵積內成纖維細胞、微血管數量、膠原沉積量較A組和B組少,且膠原排列整齊,與瘢痕長軸平行.瘢痕增生指數(HI):A組3.17±0.26,B組2.46±0.19,C組1.91±0.21,D組1.90±0.23;成纖維細胞密度(NA):A組5836.70±527.03,B組4128.73±387.66,C組3207.59±439.17,D組3200.28±421.48;膠原纖維的麵密度(AA):A組45.38±5.83,B組36.57±6.84,C組28.09±3.82,D組28.07±3.47.A組與B、C、D組比較,HI、NA、AA值差異均有統計學意義(P＜0.01);B組與C、D組比較,HI、NA、AA值差異均有統計學意義(P＜0.01);C組與D組比較,HI、NA、AA值差異無統計學意義(P＞0.05).結論 維A痠塗膜劑可以抑製兔耳早期瘢痕增生,可為防治瘢痕提供一種新的外用方法.
목적 연구유A산도막제대토이조기증생성반흔적영향,탐토기방치반흔적가행성.방법 선취신서란백토24지,건립토이증생성반흔모형후,수궤분위4조,매조6지.A조:대조조,B조:도막제조,C조:0.05％유A산도막제조,D조:0.1％유A산도막제조,련속용약6주,기간관찰기록반흔대소、후도、안색、경도,6주후분별절취토이반흔조직,HE염색,효원염색(VG법),행병이학관찰、검측급분석.결과 A조반흔안색심、후이경,병명현고우피부,표면요철불평;B조、C조、D조반흔안색천,질지연,후도박,피하결절소,기중C、D조여주위정상피부접근,D조반흔표면유탈피현상.HE、VG염색중,A조효원배렬문란,유선와상결구;C조화D조단위면적내성섬유세포、미혈관수량、효원침적량교A조화B조소,차효원배렬정제,여반흔장축평행.반흔증생지수(HI):A조3.17±0.26,B조2.46±0.19,C조1.91±0.21,D조1.90±0.23;성섬유세포밀도(NA):A조5836.70±527.03,B조4128.73±387.66,C조3207.59±439.17,D조3200.28±421.48;효원섬유적면밀도(AA):A조45.38±5.83,B조36.57±6.84,C조28.09±3.82,D조28.07±3.47.A조여B、C、D조비교,HI、NA、AA치차이균유통계학의의(P＜0.01);B조여C、D조비교,HI、NA、AA치차이균유통계학의의(P＜0.01);C조여D조비교,HI、NA、AA치차이무통계학의의(P＞0.05).결론 유A산도막제가이억제토이조기반흔증생,가위방치반흔제공일충신적외용방법.
Objective To estimate the effect of tretinoin gel sheeting on early-stage hyperplastic scars in rabbit ears,and to evaluate the feasibility to prevent and treat hyperplastic scars with it.Methods The ears of 24 rabbits were used to establish a model of hyperplastic scar according to previously reported methods.Then,the rabbit ears were randomly divided into four groups:control group receiving no treatment,gel sheeting group treated with the vehicle of the tretinoin gel sheeting,0.05％ tretinoin group treated with 0.05％ tretinoin gel sheeting,0.1％ tretinoin group treated with 0.1％ tretinoin gel sheeting.All the gel sheetings were topically used twice daily for six consecutive weeks.During the treatment,the size,thickness,color and texture of scars were estimated.After six weeks of treatment,all the scar tissues were resected from the rabbit ears and subjected to hematoxylin and eosin (HE) staining and van Gieson (VG) staining.Statistical analysis was carried out by analysis of variance and rank sum test.Results The scars were deeply colored,thickened,hard and elevated with an uneven surface in the control group,but lightly colored,thinned and soft with the presence of small subcutaneous nodules in the other three groups.The surface of scars in the two tretinoin groups was similar to that of adjacent normal skin,and scaling was observed on the scar surface in the 0.1％ tretinoin group.HE and VG staining showed a disarrangement of collagen fibers with the formation of vortex-like structures in the control group.A significant decrease was noted in the number of fibroblasts and microvessels as well as amount of collagen deposition per unit cross-sectional area in the two tretinoin groups compared with the control group and gel sheeting group.Additionally,the collagen fibers were regularly arranged and parallel to the long axis of scars in the two tretinoin groups.The scar hyperplasia index (HI) was 3.173 ± 0.26,2.465 ± 0.19,1.906 ± 0.21 and 1.903 ± 0.23 in the control group,gel sheeting group,0.05％ tretinoin group and 0.1％ tretinoin group respectively,the fibroblast density (NA) was 5836.7 ± 527.03,4128.73 ± 387.66,3207.59 ± 439.17 and 3200.28 ± 421.48 respectively,and the area density of collagen fiber (AA) was 45.38 ± 5.83,36.57 ± 6.84,28.09 ± 3.82 and 28.07 ± 3.47 respectively.As far as HI,NA and AA were concerned,the control group differed significantly from the other three groups (all P ＜ 0.01),and the gel sheeting group from the two tretinoin groups (all P ＜ 0.01),but no significant difference was observed between the two tretinoin groups (all P ＞ 0.05).Conclusions Topical tretinoin gel sheeting can inhibit scar proliferation at early stage in rabbit ears,and may provide a new choice for the prevention and treatment of hyperplastic scars.
