CAJ | 학술논문

目的探讨转录因子CCAAT增强子结合蛋白α(C/EBP-α)在寻常性银屑病皮损中的表达及与角质形成细胞异常增殖及皮损严重程度间的相关性.方法用免疫组化二步法及Western印迹检测30例寻常性银屑病患者皮损和30例健康对照皮肤的表皮中C/EBP-α.免疫组化法检测Ki-67的表达,并根据Ki-67阳性表达数量计算增殖指数,Pearson相关分析法分析寻常性银屑病皮损中C/EBP-α的表达水平与角质形成细胞Ki-67增殖指数及银屑病皮损面积和严重程度指数(PASI评分)间的相关性.结果 C/EBP-α主要在角质形成细胞胞质中表达,寻常性银屑病皮损中C/EBP-α的表达较健康对照皮肤明显下调(f=7.82,P＜ 0.05).Ki-67主要在角质形成细胞胞核中表达,寻常性银屑病皮损中角质形成细胞的增殖指数明显高于健康对照皮肤(t=4.54,P＜0.05).经Pearson相关分析,寻常性银屑病皮损中C/EBP-α表达水平与增殖指数呈负相关,与PASI评分呈负相关.Western印迹结果亦显示,C/EBP-α在银屑病皮损处的表达量显著下调.结论 C/EBP-α在寻常性银屑病皮损中表达下调,可能参与了寻常性银屑病的发病过程.
목적 탐토전록인자CCAAT증강자결합단백α(C/EBP-α)재심상성은설병피손중적표체급여각질형성세포이상증식급피손엄중정도간적상관성.방법 용면역조화이보법급Western인적검측30례심상성은설병환자피손화30례건강대조피부적표피중C/EBP-α.면역조화법검측Ki-67적표체,병근거Ki-67양성표체수량계산증식지수,Pearson상관분석법분석심상성은설병피손중C/EBP-α적표체수평여각질형성세포Ki-67증식지수급은설병피손면적화엄중정도지수(PASI평분)간적상관성.결과 C/EBP-α주요재각질형성세포포질중표체,심상성은설병피손중C/EBP-α적표체교건강대조피부명현하조(f=7.82,P＜ 0.05).Ki-67주요재각질형성세포포핵중표체,심상성은설병피손중각질형성세포적증식지수명현고우건강대조피부(t=4.54,P＜0.05).경Pearson상관분석,심상성은설병피손중C/EBP-α표체수평여증식지수정부상관,여PASI평분정부상관.Western인적결과역현시,C/EBP-α재은설병피손처적표체량현저하조.결론 C/EBP-α재심상성은설병피손중표체하조,가능삼여료심상성은설병적발병과정.
Objective To detect the expression of the transcription factor CCAAT/enhancer-binding protein alpha (C/EBP-α) in the epidermis of psoriasis vulgaris lesions,and to investigate its correlation with abnormal keratinocyte proliferation and disease severity.Methods Biopsy specimens were obtained from the lesions of 30 patients with psoriasis vulgaris and normal skin of 30 healthy human controls.A two-step immunohistochemical procedure was performed to detect the expressions of C/EBP-αt and Ki-67 in these specimens,and Western blot to quantify the expression of C/EBP-α.The proliferation index of keratinocytes was calculated according to the expression intensity of Ki-67.Statistical analysis was carried out by using the SPSS 17.0 software,and Pearson correlation analysis was conducted to assess the relationship of C/EBP-α expression level with proliferation index of keratinocytes and psoriasis area and severity index (PASI) score.Results C/EBP-α was predominantly expressed in the cytoplasm of keratinocytes,while Ki-67 in the nuclei of keratinocytes.Compared with the normal skin,the psoriatic lesions showed a significantly lower expression of Ki-67 (t =7.82,P ＜ 0.05),but higher proliferation index of keratinocytes (t =4.54,P ＜ 0.05).The expression level of C/EBP-α was negatively correlated with the proliferation index of keratinocytes and PASI score in the patients (both P ＜ 0.05).Western blot also showed an obvious decrease in the expression of C/EBP-α in psoriatic lesions.Conclusions The expression of C/EBP-α is decreased in lesions of psoriasis vulgaris,which might be involved in the pathogenesis of psoriasis vulgaris.