中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
8期
555-558
,共4页
唐梦丹%郑建峰%沈亮亮%姜淼%赵敬军
唐夢丹%鄭建峰%瀋亮亮%薑淼%趙敬軍
당몽단%정건봉%침량량%강묘%조경군
曲霉菌,烟%微管蛋白%基因型
麯黴菌,煙%微管蛋白%基因型
곡매균,연%미관단백%기인형
Aspergillus fumigatus%Tubulin%Genotype
目的 探讨我国不同地域致病烟曲霉临床分离株CSP基因型分布情况,以及不同地域、不同CSP基因型烟曲霉药物敏感性有无差异.方法 对福建、上海、河北及北京四地临床分离到的112株烟曲霉分别通过形态学、温度试验及β微管蛋白分子生物学测序鉴定,再对烟曲霉进行CSP基因分型,同时用M38-A方法测定伏立康唑、伊曲康唑及两性霉素B对不同型别烟曲霉的最低抑菌浓度(MIC).结果 112株曲霉均鉴定为经典烟曲霉,共确定11个CSP基因型,其中t04A、t03、t01最多见,分别为32株、17株、24株.福建分离株以tl0、t04A、t01最常见,上海分离株以t04A、t01最常见,河北分离株以t01、t03、t04A最常见,北京分离株以t02、t04A、t01和t03最常见,t25为1个新CSP基因分型,新基因型菌株的菌落形态、生长速度、最适生长温度与其他菌株并无显著差别.不同CSP基因型烟曲霉对抗真菌药物敏感性差异无统计学意义,但福建地区分离株对伊曲康唑的MIC值低于其他三地的分离株.结论 烟曲霉临床株CSP基因型存在一定的地域特点,不同基因型的烟曲霉对抗真菌药物的敏感性差异无统计学意义,而不同地域来源烟曲霉对伊曲康唑敏感性存在差异.
目的 探討我國不同地域緻病煙麯黴臨床分離株CSP基因型分佈情況,以及不同地域、不同CSP基因型煙麯黴藥物敏感性有無差異.方法 對福建、上海、河北及北京四地臨床分離到的112株煙麯黴分彆通過形態學、溫度試驗及β微管蛋白分子生物學測序鑒定,再對煙麯黴進行CSP基因分型,同時用M38-A方法測定伏立康唑、伊麯康唑及兩性黴素B對不同型彆煙麯黴的最低抑菌濃度(MIC).結果 112株麯黴均鑒定為經典煙麯黴,共確定11箇CSP基因型,其中t04A、t03、t01最多見,分彆為32株、17株、24株.福建分離株以tl0、t04A、t01最常見,上海分離株以t04A、t01最常見,河北分離株以t01、t03、t04A最常見,北京分離株以t02、t04A、t01和t03最常見,t25為1箇新CSP基因分型,新基因型菌株的菌落形態、生長速度、最適生長溫度與其他菌株併無顯著差彆.不同CSP基因型煙麯黴對抗真菌藥物敏感性差異無統計學意義,但福建地區分離株對伊麯康唑的MIC值低于其他三地的分離株.結論 煙麯黴臨床株CSP基因型存在一定的地域特點,不同基因型的煙麯黴對抗真菌藥物的敏感性差異無統計學意義,而不同地域來源煙麯黴對伊麯康唑敏感性存在差異.
목적 탐토아국불동지역치병연곡매림상분리주CSP기인형분포정황,이급불동지역、불동CSP기인형연곡매약물민감성유무차이.방법 대복건、상해、하북급북경사지림상분리도적112주연곡매분별통과형태학、온도시험급β미관단백분자생물학측서감정,재대연곡매진행CSP기인분형,동시용M38-A방법측정복립강서、이곡강서급량성매소B대불동형별연곡매적최저억균농도(MIC).결과 112주곡매균감정위경전연곡매,공학정11개CSP기인형,기중t04A、t03、t01최다견,분별위32주、17주、24주.복건분리주이tl0、t04A、t01최상견,상해분리주이t04A、t01최상견,하북분리주이t01、t03、t04A최상견,북경분리주이t02、t04A、t01화t03최상견,t25위1개신CSP기인분형,신기인형균주적균락형태、생장속도、최괄생장온도여기타균주병무현저차별.불동CSP기인형연곡매대항진균약물민감성차이무통계학의의,단복건지구분리주대이곡강서적MIC치저우기타삼지적분리주.결론 연곡매림상주CSP기인형존재일정적지역특점,불동기인형적연곡매대항진균약물적민감성차이무통계학의의,이불동지역래원연곡매대이곡강서민감성존재차이.
Objective To describe the CSP genotypic profile in clinical isolates of Aspergillus fumigates from different regions of China,and to investigate if there is a difference in antifungal susceptibility among A.fumigates of different CSP genotypes and from different regions.Methods Totally,112 A.fumigates strains clinically isolated from Fujian,Shanghai,Hebei and Beijing were included in this study,and identified according to macro-and micro-morphological characters,growth temperature and β-tubulin sequence.Classic A.fumigatus strains were typed according to CSP gene sequence.The minimal inhibitory concentrations (MICs) of voriconazole,itraconazole and amphotericin B to A.fumigates were determined in accordance with the National Committee for Clinical Laboratory Standards (NCCLS) M38-A protocol.Results All the strains were identified as classic A.fumigates,and fall into 11 CSP genotypes.The most common genotypes were t04A (n =32),t03 (n =17) and t01 (n =24) in all the strains,tl0,t04A and t01 in Fujian,t04A and t01 in Shanghai,t01,t03 and t04A in Hebei,t02,t04A,t01and t03 in Beijing.One A.fumigatus strain was identified as a new CSP type t25 in Fujian,which showed no obvious difference in morphology,growth rate or appropriate growth temperature from the other CSP genotypes of A.fumigatus strains.No statistical difference was found in the susceptibility to amphotericin B,itraconazole or fluconazole among different genotypes of A.fumigates,whereas the MICs of itraconazole were significantly lower in A.fumigates isolates from Fujian than in those from the other three regions.Conclusions The CSP genotypic profile of A.fumigates varies in clinical isolates from different regions.No significant difference is observed in the susceptibility to amphotericin B,itraconazole or fluconazole among different CSP genotypes of A.fumigates,but the susceptibility to itraconazole is somewhat different between A.fumigates strains from different regions.
