中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
8期
593-595
,共3页
娄银飞%马丽俐%郑明警%周慧%方一妙
婁銀飛%馬麗俐%鄭明警%週慧%方一妙
루은비%마려리%정명경%주혜%방일묘
龙胆属%角蛋白细胞%细胞增殖%细胞凋亡%受体,表皮生长因子
龍膽屬%角蛋白細胞%細胞增殖%細胞凋亡%受體,錶皮生長因子
룡담속%각단백세포%세포증식%세포조망%수체,표피생장인자
Gentian%Keratinocytes%Cell proliferation%Apoptosis%Receptor,epidermal growth factor
目的 探讨龙胆草提取物对表皮生长因子(EGF)刺激后HaCaT细胞增殖和凋亡及表皮生长因子受体(EGFR)磷酸化的影响.方法 取0～ 50g/L龙胆草提取物作用于EGF刺激后的HaCaT细胞24 h,噻唑蓝法检测细胞增殖率,流式细胞仪检测细胞凋亡率,Western印迹法检测磷酸化EGFR.结果 龙胆草提取物浓度依赖性地抑制EGF刺激后HaCaT细胞的增殖(r=-0.991,P＜0.01),促进EGF刺激后HaCaT细胞的凋亡(r=0.996,P＜ 0.05).同时,龙胆草提取物使EGF刺激后的HaCaT细胞EGFR的磷酸化水平降低,该作用随着药物浓度的增加而增加.结论 龙胆草可能通过降低EGFR的磷酸化,阻断相关细胞内信号通路来抑制角质形成细胞的过度增殖并促进细胞的凋亡.
目的 探討龍膽草提取物對錶皮生長因子(EGF)刺激後HaCaT細胞增殖和凋亡及錶皮生長因子受體(EGFR)燐痠化的影響.方法 取0～ 50g/L龍膽草提取物作用于EGF刺激後的HaCaT細胞24 h,噻唑藍法檢測細胞增殖率,流式細胞儀檢測細胞凋亡率,Western印跡法檢測燐痠化EGFR.結果 龍膽草提取物濃度依賴性地抑製EGF刺激後HaCaT細胞的增殖(r=-0.991,P＜0.01),促進EGF刺激後HaCaT細胞的凋亡(r=0.996,P＜ 0.05).同時,龍膽草提取物使EGF刺激後的HaCaT細胞EGFR的燐痠化水平降低,該作用隨著藥物濃度的增加而增加.結論 龍膽草可能通過降低EGFR的燐痠化,阻斷相關細胞內信號通路來抑製角質形成細胞的過度增殖併促進細胞的凋亡.
목적 탐토룡담초제취물대표피생장인자(EGF)자격후HaCaT세포증식화조망급표피생장인자수체(EGFR)린산화적영향.방법 취0～ 50g/L룡담초제취물작용우EGF자격후적HaCaT세포24 h,새서람법검측세포증식솔,류식세포의검측세포조망솔,Western인적법검측린산화EGFR.결과 룡담초제취물농도의뢰성지억제EGF자격후HaCaT세포적증식(r=-0.991,P＜0.01),촉진EGF자격후HaCaT세포적조망(r=0.996,P＜ 0.05).동시,룡담초제취물사EGF자격후적HaCaT세포EGFR적린산화수평강저,해작용수착약물농도적증가이증가.결론 룡담초가능통과강저EGFR적린산화,조단상관세포내신호통로래억제각질형성세포적과도증식병촉진세포적조망.
Objective To evaluate the effect of Chinese gentian extracts on the proliferation of,apoptosis and phosphorylation of epidermal growth factor receptor (EGFR) in HaCaT cells induced by epidermal growth factor (EGF).Methods Methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate the proliferation of HaCaT cells pretreated with EGF of 20 μg/L for 24 hours followed by 24 hours of treatment with various concentrations of Chinese gentian extracts.Flow cytometry was carried out to detect apoptosis in HaCaT cells pretreated with EGF of 20 μg/L for 24 hours followed by 4 hours of treatment with different concentrations of Chinese gentian extracts.Western blot was conducted to measure the level of phosphorylated EGFR in HaCaT cells treated with different concentrations of Chinese gentian extracts for 24 hours followed by treatment with EGF of 20 μg/L for 10 minutes.Results Chinese gentian extracts inhibited the proliferation (r =-0.991,P ＜ 0.01),but promoted the apoptosis (r =0.996,P ＜ 0.05) of HaCaT cells induced by EGF in a dose-dependent manner.At the same time,the extracts suppressed the phosphorylation of EGFR in HaCaT cells induced by EGF,and the suppressing effect increased with the rise in the concentration of the extracts.Conclusions Chinese gentian may inhibit the proliferation,but promote the apoptosis of keratinocytes by decreasing EGFR phosphorylation and blocking relevant intracellular signaling pathways.
