CAJ | 학술논문

目的探讨淋球菌rpsE基因突变与淋球菌大观霉素耐药的相关性.方法对临床分离的4株大观霉素耐药的淋球菌株(MIC128 μg/ml、256 μg/ml)的rpsE基因进行PCR扩增测序分析,寻找可能的突变位点,通过DNA转化技术将含有突变基因的细菌基因组DNA转化入敏感的淋球菌株,检测转化成功的淋球菌的MIC并进行PCR扩增测序,分析发现的突变位点与淋球菌大观霉素耐药的相关性.结果 4株大观霉素耐药的菌株均发现rpsE基因A70C(Thr24Pro)突变,而16S rRNA大观霉素耐药决定区(SRDR)未发现任何突变;大观霉素敏感菌株的16S rRNA和rpsE基因均未发现突变.转化后获得的大观霉素耐药淋球菌中亦发现了同样的rpsE基因突变.结论 rpsE基因单点突变与淋球菌对大观霉素耐药相关.
목적 탐토림구균rpsE기인돌변여림구균대관매소내약적상관성.방법 대림상분리적4주대관매소내약적림구균주(MIC128 μg/ml、256 μg/ml)적rpsE기인진행PCR확증측서분석,심조가능적돌변위점,통과DNA전화기술장함유돌변기인적세균기인조DNA전화입민감적림구균주,검측전화성공적림구균적MIC병진행PCR확증측서,분석발현적돌변위점여림구균대관매소내약적상관성.결과 4주대관매소내약적균주균발현rpsE기인A70C(Thr24Pro)돌변,이16S rRNA대관매소내약결정구(SRDR)미발현임하돌변;대관매소민감균주적16S rRNA화rpsE기인균미발현돌변.전화후획득적대관매소내약림구균중역발현료동양적rpsE기인돌변.결론 rpsE기인단점돌변여림구균대대관매소내약상관.
Objective To evaluate the relationship between spectinomycin resistance in Neisseria gonorrhoeae and mutations in the rpsE gene.Methods Genomic DNA was extracted from 4 clinical isolates of Neisseria gonorrhoeae with different levels of spectinomycin resistance.Then,PCR was performed to amplify the entire rpsE gene and the spectinomycin resistance-determining region (SRDR) in the 16S rRNA gene followed by direct sequencing.Two spectinomycin-sensitive Neisseria gonorrhoeae strains were transformed with the genomic DNA containing the mutant rpsE gene.Subsequently,the susceptibility of the transformants to spectinomycin was determined,and PCR was performed to amplify the rpsE and 16S rRNA genes in the transformants followed by sequencing.Results All the 4 spectinomycin-resistant Neisseria gonorrhoeae strains harbored an A70C transversion in the rpsE gene,but no abnormality in the SRDR of the 16S rRNA gene.No mutations were detected in the spectinomycin-sensitive Neisseria gonorrhoeae strains.The A70C transversion in the rpsE gene was also detected in the two Neisseria gonorrhoeae transformants with spectinomycin resistance.Conclusion The A70C point mutation within the rpsE gene is associated with spectinomycin resistance in Neisseria gonorrhoeae.