CAJ | 학술논문

目的探讨人乳头瘤病毒型别、载量与寻常疣临床特征的相关性.方法提取48例寻常疣组织DNA;正反向测序通用引物PCR扩增片段,通过blast比对分型,并通过型别特异性引物PCR来验证和补充测序结果;实时荧光定量PCR相对定量ACt法检测HPV病毒载量;HE染色法观察病理变化.结果 35例HPV PCR检测阳性的标本中HPV 7型32例;HPV 57型1例,为四肢末端多发;HPV 2和HPV 7混合感染2例,为头面部、躯干多发病例.单发组与多发组、病程＜6个月组与病程6个月至1年组病毒载量、空泡化细胞数目无明显差异,1年后病毒载量有下降趋势,病程＞2年的皮损,角化过度更明显,空泡化细胞减少.结论寻常疣的HPV感染型别主要是HPV 7;HPV 7型感染好发于头面部;对于病程1年内的寻常疣,HPV病毒载量及空泡化细胞数目与其数目、病程长短无明显相关性.
목적 탐토인유두류병독형별、재량여심상우림상특정적상관성.방법 제취48례심상우조직DNA;정반향측서통용인물PCR확증편단,통과blast비대분형,병통과형별특이성인물PCR래험증화보충측서결과;실시형광정량PCR상대정량ACt법검측HPV병독재량;HE염색법관찰병리변화.결과 35례HPV PCR검측양성적표본중HPV 7형32례;HPV 57형1례,위사지말단다발;HPV 2화HPV 7혼합감염2례,위두면부、구간다발병례.단발조여다발조、병정＜6개월조여병정6개월지1년조병독재량、공포화세포수목무명현차이,1년후병독재량유하강추세,병정＞2년적피손,각화과도경명현,공포화세포감소.결론 심상우적HPV감염형별주요시HPV 7;HPV 7형감염호발우두면부;대우병정1년내적심상우,HPV병독재량급공포화세포수목여기수목、병정장단무명현상관성.
Objective To study the association between human papillomavirus (HPV) genotypes and viral load and clinical features of verruca vulgaris.Methods Tissue samples were collected from 48 outpatients with verruca vulgaris,and DNA was extracted from these tissue samples.To determine the genotype of HPV,PCR was performed to amplify the L1 fragment of HPV with universal primers followed by bidirectional sequencing and BLAST.The genotyping results were validated by PCR with type-specific primers.Real-time fluorescence-based quantitative PCR was conducted to measure the viral load of HPV,and hematoxylin and eosin (HE) staining to observe histological changes in these tissue specimens.Results The L1 fragment of HPV was amplified from 35 out of the 48 tissue specimens.Of the 35 L1-positive specimens,32 harbored HPV 7,1 harbored HPV 57,and 2 harbored both HPV 2 and HPV 7.Multiple lesions were observed on extremities in the patient infected with HPV 57,but on the head,face and trunk in the patients coinfected with HPV 2 and HPV 7.There were no significant differences in HPV viral load or vacuolated cell number between patients with single lesions and those with multiple lesions,or between patients with a clinical course of ＜ 6 months and those with a clinical course of 6-12 months.However,HPV viral load tended to decrease one year after the onset,and there was pronounced hyperkeratosis and less vacuolated cells in lesions of long duration (more than 2 years) compared with those of short duration (less than 2 years).Conclusions HPV 7 appears to be the most common HPV genotype associated with verruca vulgaris,and HPV 7 infection usually occurs on the head and face.For verruca vulgaris of less than 1 year,neither HPV viral load nor vacuolated cell number is associated with the count or clinical course of warts.