中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
9期
637-641
,共5页
黎钊%王平%侯秀丽%刘东银%王媚%郑俊惠
黎釗%王平%侯秀麗%劉東銀%王媚%鄭俊惠
려쇠%왕평%후수려%류동은%왕미%정준혜
蕈样肉芽肿%T淋巴细胞,调节性%Th17细胞
蕈樣肉芽腫%T淋巴細胞,調節性%Th17細胞
심양육아종%T림파세포,조절성%Th17세포
Mycosis fungoides%T lymphocytes,regulatory%Th17 cells
目的 探讨调节性T(Treg)细胞和Th17细胞在蕈样肉芽肿不同分期中的变化.方法 流式细胞仪检测28例蕈样肉芽肿、13例大斑块型副银屑病、17例扁平苔藓患者及10例健康对照外周血Treg细胞和Th17细胞百分率,同时应用免疫组化法检测40例蕈样肉芽肿、13例副银屑病、17例扁平苔藓及10例健康对照蜡块组织中叉头状转录因子P3(FOXP3)和白细胞介素17(IL-17)的表达.结果 蕈样肉芽肿、副银屑病、扁平苔藓患者外周血Treg细胞百分率分别为(8.09±1.68)％,(6.53±1.67)％,(2.84±1.16)％较健康对照[(1.01±0.35)％]升高,差异均有统计学意义.蕈样肉芽肿、副银屑病患者外周血Treg细胞百分率亦高于扁平苔藓(均P＜ 0.05);蕈样肉芽肿与副银屑病患者差异无统计学意义(P＞0.05).外周血Th17细胞百分率在蕈样肉芽肿较副银屑病、扁平苔藓患者比健康人升高[(3.22±0.82)％比(2.46±0.79)％,(1.38±0.47)％和(0.59±0.30)％,均P＜ 0.05].FOXP3阳性率在蕈样肉芽肿、副银屑病及扁平苔藓均高于正常皮肤组织[(14.94±4.46)％,(11.95±4.72)％,(6.32±2.81)％比(3.43±1.79)％,均P＜ 0.05],蕈样肉芽肿及副银屑病比扁平苔藓高(均P＜ 0.05),蕈样肉芽肿比副银屑病差异无统计学意义(P＞0.05).IL-17阳性率在蕈样肉芽肿、副银屑病、扁平苔藓和正常组织中分别为(15.89±4.27)％,(12.02±3.34)％,(4.84±1.93)％和(2.62±0.89)％,其中,蕈样肉芽肿均高于副银屑病、扁平苔藓组织及正常组织(均P＜ 0.05).蕈样肉芽肿、副银屑病外周血Th 17/Treg细胞比率比扁平苔藓、健康对照低(0.41±0.11,0.39±0.12比0.50±0.06,0.57±0.19(均P＜0.05).早期蕈样肉芽肿Th17细胞与Treg细胞呈正相关(r=0.423,P＜ 0.05),肿瘤期蕈样肉芽肿Th17细胞有所下降,而Treg细胞继续升高,但蕈样肉芽肿各期差异无统计学意义,且二者在肿瘤期无相关性.结论 Treg和Th17细胞失衡可能参与了蕈样肉芽肿的发生与发展.
目的 探討調節性T(Treg)細胞和Th17細胞在蕈樣肉芽腫不同分期中的變化.方法 流式細胞儀檢測28例蕈樣肉芽腫、13例大斑塊型副銀屑病、17例扁平苔蘚患者及10例健康對照外週血Treg細胞和Th17細胞百分率,同時應用免疫組化法檢測40例蕈樣肉芽腫、13例副銀屑病、17例扁平苔蘚及10例健康對照蠟塊組織中扠頭狀轉錄因子P3(FOXP3)和白細胞介素17(IL-17)的錶達.結果 蕈樣肉芽腫、副銀屑病、扁平苔蘚患者外週血Treg細胞百分率分彆為(8.09±1.68)％,(6.53±1.67)％,(2.84±1.16)％較健康對照[(1.01±0.35)％]升高,差異均有統計學意義.蕈樣肉芽腫、副銀屑病患者外週血Treg細胞百分率亦高于扁平苔蘚(均P＜ 0.05);蕈樣肉芽腫與副銀屑病患者差異無統計學意義(P＞0.05).外週血Th17細胞百分率在蕈樣肉芽腫較副銀屑病、扁平苔蘚患者比健康人升高[(3.22±0.82)％比(2.46±0.79)％,(1.38±0.47)％和(0.59±0.30)％,均P＜ 0.05].FOXP3暘性率在蕈樣肉芽腫、副銀屑病及扁平苔蘚均高于正常皮膚組織[(14.94±4.46)％,(11.95±4.72)％,(6.32±2.81)％比(3.43±1.79)％,均P＜ 0.05],蕈樣肉芽腫及副銀屑病比扁平苔蘚高(均P＜ 0.05),蕈樣肉芽腫比副銀屑病差異無統計學意義(P＞0.05).IL-17暘性率在蕈樣肉芽腫、副銀屑病、扁平苔蘚和正常組織中分彆為(15.89±4.27)％,(12.02±3.34)％,(4.84±1.93)％和(2.62±0.89)％,其中,蕈樣肉芽腫均高于副銀屑病、扁平苔蘚組織及正常組織(均P＜ 0.05).蕈樣肉芽腫、副銀屑病外週血Th 17/Treg細胞比率比扁平苔蘚、健康對照低(0.41±0.11,0.39±0.12比0.50±0.06,0.57±0.19(均P＜0.05).早期蕈樣肉芽腫Th17細胞與Treg細胞呈正相關(r=0.423,P＜ 0.05),腫瘤期蕈樣肉芽腫Th17細胞有所下降,而Treg細胞繼續升高,但蕈樣肉芽腫各期差異無統計學意義,且二者在腫瘤期無相關性.結論 Treg和Th17細胞失衡可能參與瞭蕈樣肉芽腫的髮生與髮展.
목적 탐토조절성T(Treg)세포화Th17세포재심양육아종불동분기중적변화.방법 류식세포의검측28례심양육아종、13례대반괴형부은설병、17례편평태선환자급10례건강대조외주혈Treg세포화Th17세포백분솔,동시응용면역조화법검측40례심양육아종、13례부은설병、17례편평태선급10례건강대조사괴조직중차두상전록인자P3(FOXP3)화백세포개소17(IL-17)적표체.결과 심양육아종、부은설병、편평태선환자외주혈Treg세포백분솔분별위(8.09±1.68)％,(6.53±1.67)％,(2.84±1.16)％교건강대조[(1.01±0.35)％]승고,차이균유통계학의의.심양육아종、부은설병환자외주혈Treg세포백분솔역고우편평태선(균P＜ 0.05);심양육아종여부은설병환자차이무통계학의의(P＞0.05).외주혈Th17세포백분솔재심양육아종교부은설병、편평태선환자비건강인승고[(3.22±0.82)％비(2.46±0.79)％,(1.38±0.47)％화(0.59±0.30)％,균P＜ 0.05].FOXP3양성솔재심양육아종、부은설병급편평태선균고우정상피부조직[(14.94±4.46)％,(11.95±4.72)％,(6.32±2.81)％비(3.43±1.79)％,균P＜ 0.05],심양육아종급부은설병비편평태선고(균P＜ 0.05),심양육아종비부은설병차이무통계학의의(P＞0.05).IL-17양성솔재심양육아종、부은설병、편평태선화정상조직중분별위(15.89±4.27)％,(12.02±3.34)％,(4.84±1.93)％화(2.62±0.89)％,기중,심양육아종균고우부은설병、편평태선조직급정상조직(균P＜ 0.05).심양육아종、부은설병외주혈Th 17/Treg세포비솔비편평태선、건강대조저(0.41±0.11,0.39±0.12비0.50±0.06,0.57±0.19(균P＜0.05).조기심양육아종Th17세포여Treg세포정정상관(r=0.423,P＜ 0.05),종류기심양육아종Th17세포유소하강,이Treg세포계속승고,단심양육아종각기차이무통계학의의,차이자재종류기무상관성.결론 Treg화Th17세포실형가능삼여료심양육아종적발생여발전.
Objective To investigate the changes of T helper type 17 (Thl7) cells and regulatory T (Treg) cells in different stages of mycosis fungoides.Methods Flow cytometry was performed to determine the percentage of Treg and Th17 cells in peripheral blood from 28 patients with mycosis fungoides (MF),13 patients with large plaque parapsoriasis (PP),17 patients with lichen planus (LP) and 10 healthy human controls,and immunohistochemistry to detect the expressions of forkhead box protein 3 (FOXP3) and interleukin (IL)-17 in tissue specimens from 40 patients with MF,13 with PP,17 with LP and 10 healthy human controls.Statistical analysis was carried out by one-way analysis of variance and Pearson correlation analysis.Results As far as the percentage of Treg cells in peripheral blood was concerned,MF,PP and LP patients were significantly higher than the healthy controls (8.09％ ± 1.68％,6.53％ ± 1.67％ and 2.84 ％ ± 1.16％ vs.1.01％ ± 0.35,all P＜ 0.05),PP patients were higher than LP patients and healthy controls (both P ＜ 0.05),and LP patients were higher than healthy controls (P ＜ 0.05).The percentage of Th17 cells in peripheral blood was significantly increased in MF patients compared with PP patients,LP patients and healthy controls (3.22％ ± 0.82％ vs.2.46％ ± 0.79％,1.38％ ± 0.47％ and 0.59％ ± 0.30％,all P ＜ 0.05).Elevated expression rate of FOXP3 was observed in MF,PP and LP lesions as compared with normal skin (14.94％ ± 4.46％,11.95％ ± 4.72％,6.32％ ± 2.81％ vs.3.43％ ± 1.79％,all P ＜ 0.05),and in MF and PP lesions compared with LP lesions (both P ＜ 0.05),but no significant difference was observed between MF and PP lesions (P ＞ 0.05).There was a significant increase in the expression rate of IL-17 in MF lesions compared with PP lesions,LP lesions and normal skin (15.89％ ± 4.27％ vs.12.02％ ± 3.34％,4.84％ ± 1.93％ and 2.62％ ± 0.89％,all P ＜ 0.05).The Th17/Treg ratio in peripheral blood was significantly lower in MF and PP patients than in LP patients and healthy controls (0.41 ± 0.11 and 0.39 ± 0.12 vs.0.50 ± 0.06 and 0.57 ± 0.19,all P ＜ 0.05).A positive correlation was observed between the proportion of Thl7 cells and Treg cells (r =0.423,P ＜ 0.05) in patients with early-stage MF,but not in those with tumor-stage MF.The proportion of Th17 cells decreased,but that of Treg cells continuously increased in patients with tumor-stage MF.However,no significant difference was noted in the proportion of Thl7 cells or Treg cells among patients with different stages of MF.Conclusion The imbalance between Treg and Th17 cells may be involved in the occurrence and development of MF.
