CAJ | 학술논문

目的探讨过氧化物酶增殖物激活受体β/δ (PPARβ/δ)在银屑病患者表皮角质形成细胞(KC)中的表达和调节因素.方法免疫组化方法检测PPARβ/δ在银屑病患者皮损及非皮损表皮中的表达.分离、培养银屑病患者非皮损区和皮损区的KC,以RT-PCR和Western印迹法分别检测银屑病患者皮损区和非皮损区KC中PPARβ/δ mRNA和蛋白质的表达水平.利用PPARβ/δ外源性激动剂GW501516及Ca2+刺激银屑病非皮损区KC,观察其对PPARβ/δ表达的调节影响.结果免疫组化显示,银屑病皮损区PPARβ/δ的表达强度显著高于正常对照组(t=19.28,P＜0.01)和非皮损区(t=23.26,P＜0.01).银屑病皮损区PPARβ/δ mRNA和蛋白质的表达水平均高于正常对照组(P＜0.01)和非皮损区(P＜ 0.01).10 ng/ml GW501516最大效能促进PPARβ/δ的表达(P＜ 0.01);1.0 mmol/L Ca2+对KC中PPARβ/δ的表达促进效应最明显(P＜0.01).结论 PPARβ/δ在银屑病患者皮损区表达显著升高,GW501516和CaCa2+能够促进角质形成细胞PPARβ/δ表达水平.
목적 탐토과양화물매증식물격활수체β/δ (PPARβ/δ)재은설병환자표피각질형성세포(KC)중적표체화조절인소.방법 면역조화방법검측PPARβ/δ재은설병환자피손급비피손표피중적표체.분리、배양은설병환자비피손구화피손구적KC,이RT-PCR화Western인적법분별검측은설병환자피손구화비피손구KC중PPARβ/δ mRNA화단백질적표체수평.이용PPARβ/δ외원성격동제GW501516급Ca2+자격은설병비피손구KC,관찰기대PPARβ/δ표체적조절영향.결과 면역조화현시,은설병피손구PPARβ/δ적표체강도현저고우정상대조조(t=19.28,P＜0.01)화비피손구(t=23.26,P＜0.01).은설병피손구PPARβ/δ mRNA화단백질적표체수평균고우정상대조조(P＜0.01)화비피손구(P＜ 0.01).10 ng/ml GW501516최대효능촉진PPARβ/δ적표체(P＜ 0.01);1.0 mmol/L Ca2+대KC중PPARβ/δ적표체촉진효응최명현(P＜0.01).결론 PPARβ/δ재은설병환자피손구표체현저승고,GW501516화CaCa2+능구촉진각질형성세포PPARβ/δ표체수평.
Objective To measure the expression of peroxisome proliferator-activated receptor β/δ (PPARβ/δ) in epidermal keratinocytes from patients with psoriasis,and to investigate its regulatory factors.Methods Tissue specimens were obtained from both lesional and non-lesional skin of 20 patients with psoriasis as well as from normal skin of 15 human controls.An immunohistochemical method was used to examine the expression of PPARβ/δ in these tissue specimens.Epidermal keratinocytes were isolated from these tissue specimens and subjected to a primary culture.After several passages of subculture,non-lesional psoriatic keratinocytes were stimulated with different concentrations of GW501516 (an agonist of PPARβ/δ,0-100 ng/ml) and Ca2+ (0-3.0 mmol/L).Reverse transcription-PCR and Western blot were performed to measure the mRNA and protein expressions of PPARβ/δ in the primary keratinocytes and stimulated keratinocytes respectively.Results Immunohistochemistry showed that the expression intensity of PPARβ/δ was significantly higher in lesional psoriatic skin than in normal control skin (t =19.28,P ＜ 0.01) and non-lesional psoriatic skin (t =23.26,P ＜ 0.01).Increased mRNA and protein levels of PPARβ/δ were observed in lesional psoriatic keratinocytes as compared to normal control keratinocytes (both P ＜0.01) and non-lesional psoriatic keratinocytes (both P ＜ 0.01).Among these stimulated non-lesional psoriatic keratinocytes,those treated with GW501516 at 10 ng/ml and those with Ca2+ of 1.0 mmol/L showed the strongest expression of PPARβ/δ (both P ＜ 0.01).Conclusions The expression of PPARβ/δ,which is higher in lesional psoriatic skin,can be enhanced by GW501516 and Ca2+ in keratinocytes.