中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
10期
728-731
,共4页
吴艳华%汤楠%蔡兰花%李其林
吳豔華%湯楠%蔡蘭花%李其林
오염화%탕남%채란화%리기림
阿魏酸%黑素细胞%细胞增殖%酪氨酸%原癌基因蛋白质c-kit
阿魏痠%黑素細胞%細胞增殖%酪氨痠%原癌基因蛋白質c-kit
아위산%흑소세포%세포증식%락안산%원암기인단백질c-kit
Ferulic acid%Melanocytes%Cell proliferation%Tyrosine%Proto-oncogene proteins c-kit
目的 探讨阿魏酸对体外培养的正常人表皮黑素细胞增殖、黑素合成、酪氨酸酶活性及c-kit、ERK蛋白表达的影响.方法 以不同浓度的阿魏酸干预体外培养的正常人表皮黑素细胞,用MTS法分别检测培养24、48、72 h后黑素细胞的增殖活性.用NaOH裂解法检测培养72 h后黑素细胞的黑素合成.用多巴氧化反应法测定培养72 h后黑素细胞酪氨酸酶活性.用Western印迹法测定培养72 h黑素细胞c-kit蛋白及ERK1/2蛋白表达水平.结果 与对照组相比,0.01、0.1、1 mg/ml阿魏酸在作用24、48、72 h后,抑制黑素细胞增殖作用的差异有统计学意义(均P＜ 0.05),其中1 mg/ml阿魏酸作用72 h抑制黑素细胞活性最高.0.01、0.1、1 mg/ml阿魏酸均能显著影响黑素合成和酪氨酸酶活性,并可降低黑素细胞中c-kit蛋白及ERK1/2蛋白表达水平,与对照组相比,差异有统计学意义(均P＜ 0.05).结论 阿魏酸可抑制培养的人表皮黑素细胞增殖、黑素合成及酪氨酸酶活性,并可下调黑素细胞c-kit蛋白及ERK蛋白的表达.
目的 探討阿魏痠對體外培養的正常人錶皮黑素細胞增殖、黑素閤成、酪氨痠酶活性及c-kit、ERK蛋白錶達的影響.方法 以不同濃度的阿魏痠榦預體外培養的正常人錶皮黑素細胞,用MTS法分彆檢測培養24、48、72 h後黑素細胞的增殖活性.用NaOH裂解法檢測培養72 h後黑素細胞的黑素閤成.用多巴氧化反應法測定培養72 h後黑素細胞酪氨痠酶活性.用Western印跡法測定培養72 h黑素細胞c-kit蛋白及ERK1/2蛋白錶達水平.結果 與對照組相比,0.01、0.1、1 mg/ml阿魏痠在作用24、48、72 h後,抑製黑素細胞增殖作用的差異有統計學意義(均P＜ 0.05),其中1 mg/ml阿魏痠作用72 h抑製黑素細胞活性最高.0.01、0.1、1 mg/ml阿魏痠均能顯著影響黑素閤成和酪氨痠酶活性,併可降低黑素細胞中c-kit蛋白及ERK1/2蛋白錶達水平,與對照組相比,差異有統計學意義(均P＜ 0.05).結論 阿魏痠可抑製培養的人錶皮黑素細胞增殖、黑素閤成及酪氨痠酶活性,併可下調黑素細胞c-kit蛋白及ERK蛋白的錶達.
목적 탐토아위산대체외배양적정상인표피흑소세포증식、흑소합성、락안산매활성급c-kit、ERK단백표체적영향.방법 이불동농도적아위산간예체외배양적정상인표피흑소세포,용MTS법분별검측배양24、48、72 h후흑소세포적증식활성.용NaOH렬해법검측배양72 h후흑소세포적흑소합성.용다파양화반응법측정배양72 h후흑소세포락안산매활성.용Western인적법측정배양72 h흑소세포c-kit단백급ERK1/2단백표체수평.결과 여대조조상비,0.01、0.1、1 mg/ml아위산재작용24、48、72 h후,억제흑소세포증식작용적차이유통계학의의(균P＜ 0.05),기중1 mg/ml아위산작용72 h억제흑소세포활성최고.0.01、0.1、1 mg/ml아위산균능현저영향흑소합성화락안산매활성,병가강저흑소세포중c-kit단백급ERK1/2단백표체수평,여대조조상비,차이유통계학의의(균P＜ 0.05).결론 아위산가억제배양적인표피흑소세포증식、흑소합성급락안산매활성,병가하조흑소세포c-kit단백급ERK단백적표체.
Objective To evaluate the in vitro effect of ferulic acid on the proliferation of,as well as melanin synthesis,tyrosinase activity and expressions of c-kit and ERK proteins in cultured normal human epidermal melanocytes.Methods Cultured normal human epidermal melanocytes were treated with various concentrations of ferulic acid for different durations,and those remaining untreated served as the control.Then,3-(4,5-dimethylthiazol2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,inner salt (MTS) assay was performed to estimate cell proliferative activity at 24,48 and 72 hours,sodium hydroxide solubilization method to quantify melanin content in melanocytes at 72 hours,dopa oxidation assay to evaluate tyrosinase activity at 72 hours,Western blot to measure the expressions of c-kit and ERK1/2 proteins at 72 hours.Results Cellular proliferative activity was significantly inhibited in melanocytes treated with ferulic acid of 0.01,0.1 and 1 mg/ml for 24,48 and 72 hours compared with untreated melanocytes (all P ＜ 0.05),and the 72-hour treatment with ferulic acid of 1 mg/ml showed the strongest inhibitory effect.Ferulic acid at 0.01,0.1 and 1 mg/ml all markedly suppressed melanin synthesis and tyrosinase activity,decreased the expressions of c-kit and ERK1/2 proteins in melanocytes,with significant differences in these parameters between ferulic acid-treated and untreated melanocytes (all P ＜ 0.05).Conclusions Ferulic acid could downregulate the proliferation of,as well as melanin synthesis,tyrosinase activity,and expressions of c-kit and ERK proteins in cultured human epidermal melanocytes.
