中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2014年
11期
796-799
,共4页
任纪祯%陈振雨%杨廷%万金娥%赵明
任紀禎%陳振雨%楊廷%萬金娥%趙明
임기정%진진우%양정%만금아%조명
高压氧%瘢痕%缺氧诱导因子1,α亚基%胰岛素样生长因子-Ⅰ受体
高壓氧%瘢痕%缺氧誘導因子1,α亞基%胰島素樣生長因子-Ⅰ受體
고압양%반흔%결양유도인자1,α아기%이도소양생장인자-Ⅰ수체
Hyperbaric oxygenation%Cicatrix%Hypoxia-inducible factor 1,alpha subunit%Receptor,IGF type 1
目的 探讨高压氧对兔耳增生性瘢痕的形成及瘢痕组织中缺氧诱导因子1α(HIF-1α)、胰岛素样生长因子Ⅰ受体(IGF-1R)表达的影响.方法 选取20只新西兰大白兔建立兔耳瘢痕模型,随机分成实验组和对照组,实验组根据给予高压氧时间不同分为7d、14 d、21 d及28 d组,每组4只共48个创面,实验组术后立即给予高压氧处理,对照组处于常氧压空气环境中.术后第29天切取瘢痕组织进行苏木精-伊红(HE)染色,观察形态学差异,计算瘢痕增生指数;免疫组化法检测HIF-1α、IGF-1R的表达.结果 HE染色可见实验组成纤维细胞数量及胶原纤维含量较对照组明显减少.对照组瘢痕增生指数为4.28±0.22,7d、14 d、21 d及28 d实验组分别为3.64±0.29、3.46±0.21、3.29±0.21、3.16±0.15,各组间差异有统计学意义(F=77.70,P＜0.05).免疫组化检测可见,各实验组HIF-1α、IGF-1R的表达量均较对照组显著降低(P＜0.01),14 d组两因子表达量较7d组、21 d组较14 d组减少(均P＜0.05),28 d组与21 d组比较,差异无统计学意义(均P＞0.05).结论 高压氧可降低兔耳瘢痕组织中HIF-1α、IGF-1R的表达,对兔耳增生性瘢痕的形成有明显抑制作用.
目的 探討高壓氧對兔耳增生性瘢痕的形成及瘢痕組織中缺氧誘導因子1α(HIF-1α)、胰島素樣生長因子Ⅰ受體(IGF-1R)錶達的影響.方法 選取20隻新西蘭大白兔建立兔耳瘢痕模型,隨機分成實驗組和對照組,實驗組根據給予高壓氧時間不同分為7d、14 d、21 d及28 d組,每組4隻共48箇創麵,實驗組術後立即給予高壓氧處理,對照組處于常氧壓空氣環境中.術後第29天切取瘢痕組織進行囌木精-伊紅(HE)染色,觀察形態學差異,計算瘢痕增生指數;免疫組化法檢測HIF-1α、IGF-1R的錶達.結果 HE染色可見實驗組成纖維細胞數量及膠原纖維含量較對照組明顯減少.對照組瘢痕增生指數為4.28±0.22,7d、14 d、21 d及28 d實驗組分彆為3.64±0.29、3.46±0.21、3.29±0.21、3.16±0.15,各組間差異有統計學意義(F=77.70,P＜0.05).免疫組化檢測可見,各實驗組HIF-1α、IGF-1R的錶達量均較對照組顯著降低(P＜0.01),14 d組兩因子錶達量較7d組、21 d組較14 d組減少(均P＜0.05),28 d組與21 d組比較,差異無統計學意義(均P＞0.05).結論 高壓氧可降低兔耳瘢痕組織中HIF-1α、IGF-1R的錶達,對兔耳增生性瘢痕的形成有明顯抑製作用.
목적 탐토고압양대토이증생성반흔적형성급반흔조직중결양유도인자1α(HIF-1α)、이도소양생장인자Ⅰ수체(IGF-1R)표체적영향.방법 선취20지신서란대백토건립토이반흔모형,수궤분성실험조화대조조,실험조근거급여고압양시간불동분위7d、14 d、21 d급28 d조,매조4지공48개창면,실험조술후립즉급여고압양처리,대조조처우상양압공기배경중.술후제29천절취반흔조직진행소목정-이홍(HE)염색,관찰형태학차이,계산반흔증생지수;면역조화법검측HIF-1α、IGF-1R적표체.결과 HE염색가견실험조성섬유세포수량급효원섬유함량교대조조명현감소.대조조반흔증생지수위4.28±0.22,7d、14 d、21 d급28 d실험조분별위3.64±0.29、3.46±0.21、3.29±0.21、3.16±0.15,각조간차이유통계학의의(F=77.70,P＜0.05).면역조화검측가견,각실험조HIF-1α、IGF-1R적표체량균교대조조현저강저(P＜0.01),14 d조량인자표체량교7d조、21 d조교14 d조감소(균P＜0.05),28 d조여21 d조비교,차이무통계학의의(균P＞0.05).결론 고압양가강저토이반흔조직중HIF-1α、IGF-1R적표체,대토이증생성반흔적형성유명현억제작용.
Objective To evaluate the effects of hyperbaric oxygen on the expressions of hypoxia-inducible factor 1α (HIF-1α) and insulin-like growth factor-1 receptor (IGF-1R) in the formation of hyperplastic scar in rabbit ears.Methods The ears of 20 New Zealand rabbits were used to construct an animal model for hyperplastic scar by operation.After the establishment of scar models,the rabbits were randomly divided into 4 experimental groups and one control group with 4 mice (48 wound surfaces) in each group.The mice in the 4 experimental groups were treated with hyperbaric oxygen for 7,14,21 and 28 days,respectively,and those in the control group remained in normoxic environment after operation.Scar tissues were resected from all the rabbit ears on day 29 after operation.Hematoxylin and eosin (HE) staining was conducted for the observation of morphological changes and calculation of scar elevation index,and immunohistochemistry to measure the expressions of HIF-1α and IGF-1R.Statistical analysis was carried out by one-way analysis of variance followed by least significant difference t-test.Results HE staining showed that both the number of fibroblasts and amount of collagen fibers were significantly reduced in the experimental groups compared with the control group.Scar elevation index was 4.28 ± 0.22 in the control group,3.64 ± 0.29,3.46 ± 0.21,3.29 ± 0.21,3.16 ± 0.15 in the 7-,14-,21-and 28-day experimental groups respectively,with significant differences among these groups (F =77.70,P ＜ 0.05).The expressions of HIF-1α and IGF-1R were significantly lower in these experimental groups than in the control group (all P ＜ 0.01),lower in the 14-day group than in the 7-day group (P ＜ 0.05),and lower in the 21-day group than in the 14-day group (P ＜ 0.05),with no significant differences between the 28-day group and 21-day group (both P ＞ 0.05).Conclusion Hyperbaric oxygen can effectively down-regulate the expressions of HIF-1α and IGF-1R in scar tissue,and significantly inhibit the formation of hyperplastic scar in rabbit ears.
