CAJ | 학술논문

目的探讨蛋白质芯片技术在乳腺癌新辅助化疗敏感与耐药相关蛋白筛查中的价值.方法提取10例TA方案(蒽环类+紫杉类)新辅助化疗患者术前空芯针穿刺标本的总蛋白;根据Miller & Payne分级将患者分为两组,将grade 4～5合并为化疗敏感组,grade 1～2合并为化疗耐药组,每组各有5例患者;应用抗体芯片与提取蛋白进行杂交,检测656种肿瘤相关蛋白表达水平;运用上述10例新辅助化疗患者的蛋白质表达结果筛选差异蛋白,并进行聚类分析.结果空芯针穿刺活检可以为蛋白质芯片研究提供足够量的蛋白;分析化疗敏感组与化疗耐药组患者的蛋白表达谱差异,筛选出差异蛋白61个;以此差异蛋白为基础的聚类分析可将此两组乳腺癌标本聚成两类,准确率100%.结论以蛋白质芯片技术为基础的分子分型对于预测乳腺癌新辅助化疗的敏感性有潜在的临床应用价值,但我们需要进一步的大样本研究验证此模型的稳定性和准确性.
목적 탐토단백질심편기술재유선암신보조화료민감여내약상관단백사사중적개치.방법 제취10례TA방안(은배류+자삼류)신보조화료환자술전공심침천자표본적총단백;근거Miller & Payne분급장환자분위량조,장grade 4～5합병위화료민감조,grade 1～2합병위화료내약조,매조각유5례환자;응용항체심편여제취단백진행잡교,검측656충종류상관단백표체수평;운용상술10례신보조화료환자적단백질표체결과사선차이단백,병진행취류분석.결과 공심침천자활검가이위단백질심편연구제공족구량적단백;분석화료민감조여화료내약조환자적단백표체보차이,사선출차이단백61개;이차차이단백위기출적취류분석가장차량조유선암표본취성량류,준학솔100%.결론 이단백질심편기술위기출적분자분형대우예측유선암신보조화료적민감성유잠재적림상응용개치,단아문수요진일보적대양본연구험증차모형적은정성화준학성.
Objective To screen the proteins that are related to the sensitivity or resistance of neoadjuvant chemotherapy in breast cancer and to provide molecular basis on the prediction model.Methods 10 patients were included in this study,who received neo-adjuvant taxane and anthracyclinebased chemotherapy,total protein was extracted from core needle biopsy samples of which 5 were sensitive to neo-adjuvant chemotherapy and 5 were resistant to neo-adjuvant chemotherapy.The sensitivity criterion was based on Miller & Payne grading,grade 4 and grade 5 were considered as sensitive,grade 1 and grade 2 were considered as resistant.Protein profiling was analyzed by antibody microarray,then screening of sensitive and resistant related proteins and clustering analysis were conducted.Results Core needle biopsy can provide sufficient protein to conduct the antibody microarray analysis,61 differentially expressed proteins were identified,10 patients were grouped as sensitive or resistant to neo-adjuvant chemotherapy by clustering analysis with an accuracy of 100%.Conclusion Molecular subtype of breast cancer based on proteomic analysis has a potential value to predict the sensitivity to neo-adjuvant chemotherapy.