CAJ | 학술논문

目的探讨胆囊癌组织胞内氯离子通道蛋白1(chloride intracellular channel 1,CLIC1)蛋白表达与胆囊癌侵袭转移的关系. 方法应用免疫组织化学SP染色法及Western blot技术,检测36例胆囊癌和相应癌旁组织中CLIC1蛋白的表达.采用Spearman等级相关分析、x2检验对结果进行分析.结果免疫组化结果提示,CLIC1蛋白高表达于胆囊癌组织,癌旁组织中CLIC1蛋白表达较少,阳性表达率分别为81％ (29/36)和44％ (16/36),差异有统计学意义(P＜0.05).胆囊癌组织CLIC1表达与患者性别和年龄无关(P＞0.05),而与肿瘤的淋巴转移、浸润深度、TNM分期和分化程度相关(P＜0.05).Western blot结果显示,CLIC1的特异性条带出现在Marker标准相对分子质量27 000左右,CLIC1在胆囊癌的阳性率高于癌旁组织(P＜0.05).结论 CLIC1可能在胆囊癌的发生发展过程中发挥重要的作用,CLIC1蛋白的高表达与胆囊癌的侵袭转移密切相关.
목적 탐토담낭암조직포내록리자통도단백1(chloride intracellular channel 1,CLIC1)단백표체여담낭암침습전이적관계. 방법 응용면역조직화학SP염색법급Western blot기술,검측36례담낭암화상응암방조직중CLIC1단백적표체.채용Spearman등급상관분석、x2검험대결과진행분석.결과 면역조화결과제시,CLIC1단백고표체우담낭암조직,암방조직중CLIC1단백표체교소,양성표체솔분별위81％ (29/36)화44％ (16/36),차이유통계학의의(P＜0.05).담낭암조직CLIC1표체여환자성별화년령무관(P＞0.05),이여종류적림파전이、침윤심도、TNM분기화분화정도상관(P＜0.05).Western blot결과현시,CLIC1적특이성조대출현재Marker표준상대분자질량27 000좌우,CLIC1재담낭암적양성솔고우암방조직(P＜0.05).결론 CLIC1가능재담낭암적발생발전과정중발휘중요적작용,CLIC1단백적고표체여담낭암적침습전이밀절상관.
Objective To investigate the correlation between the CLIC1 protein expression with the invasion and metastasis in gallbladder cancer.Methods The expressions of CLIC1 was detected in 36 matching-samples including primary gallbladder cancer tissues and paratumor normal tissues by immunohistochemistry and Western blot.All data were analyzed via Spearman rank correlation coefficient and chi-square test.Results The over-expression of CLIC1 was found in 81％ (29/36)of the gallbladder cancer tissues,whereas in 44％ (16/36) of paratumor normal tissues.There were significant differences in the expression of CLIC1 between them (P ＜0.05).The expression of CLIC1 did not correlate with age and sex of patients (P ＞ 0.05),but with tumor differentiation and lymph node metastasis (P ＜ 0.05).Western blot displayed clear bands of CLICI about 27 000 by molecular weight marker Mix,and there was a significant difference between the high expressions of CLIC1 in gallbladder cancer and the low expressions in paratumor normal tissues(P ＜ 0.05).Conclusions CLIC1 may play an important role in tumorigenesis of gallbladder cancer.The invasion and metastasis of gallbladder cancer lesions are correlated with the expression of CLIC1.