中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2013年
2期
116-119
,共4页
骆成玉%杨鋆%于德明%季晓昕%赵新飞
駱成玉%楊鋆%于德明%季曉昕%趙新飛
락성옥%양윤%우덕명%계효흔%조신비
结直肠肿瘤%肿瘤转移%微RNAs%基因调控网络
結直腸腫瘤%腫瘤轉移%微RNAs%基因調控網絡
결직장종류%종류전이%미RNAs%기인조공망락
Colorectal neoplasms%Neoplasm metastasis%microRNAs%Gene regulatory networks
目的 研究结直肠癌肝脏转移微小RNA (miRNA)表达的差异及相关特性,并对结直肠癌肝脏转移miRNA对应的靶基因和生物信息学特征进行针对性分析.方法 收集10例伴有或不伴有肝转移的结直肠癌患者肿瘤组织标本.应用miRNA芯片方法对两组样本的miRNA表达差异情况进行研究,利用软件预测靶基因.进一步构建和分析肝转移结直肠癌相关的miRNA-Target转录调控网络及基因本体论功能模块.结果 芯片结果筛选出6种结直肠癌肝转移组较非转移组表达失调的miRNA(上调的miR-224、miR-1236和miR-622；下调的miR-155、miR-342-5p、miR-363).最显著上调的miR-224不但可以与其对应靶基因行使调控作用,而且可以与其他miRNA协同作用于其下游的靶基因功能团,行使相应功能.结论 miR-224作为调控网络的核心,可同时或异时性调控相关的重要基因功能团,进而完成对结直肠癌肝脏转移的转录后水平操控,在肝转移过程中起重要作用.
目的 研究結直腸癌肝髒轉移微小RNA (miRNA)錶達的差異及相關特性,併對結直腸癌肝髒轉移miRNA對應的靶基因和生物信息學特徵進行針對性分析.方法 收集10例伴有或不伴有肝轉移的結直腸癌患者腫瘤組織標本.應用miRNA芯片方法對兩組樣本的miRNA錶達差異情況進行研究,利用軟件預測靶基因.進一步構建和分析肝轉移結直腸癌相關的miRNA-Target轉錄調控網絡及基因本體論功能模塊.結果 芯片結果篩選齣6種結直腸癌肝轉移組較非轉移組錶達失調的miRNA(上調的miR-224、miR-1236和miR-622；下調的miR-155、miR-342-5p、miR-363).最顯著上調的miR-224不但可以與其對應靶基因行使調控作用,而且可以與其他miRNA協同作用于其下遊的靶基因功能糰,行使相應功能.結論 miR-224作為調控網絡的覈心,可同時或異時性調控相關的重要基因功能糰,進而完成對結直腸癌肝髒轉移的轉錄後水平操控,在肝轉移過程中起重要作用.
목적 연구결직장암간장전이미소RNA (miRNA)표체적차이급상관특성,병대결직장암간장전이miRNA대응적파기인화생물신식학특정진행침대성분석.방법 수집10례반유혹불반유간전이적결직장암환자종류조직표본.응용miRNA심편방법대량조양본적miRNA표체차이정황진행연구,이용연건예측파기인.진일보구건화분석간전이결직장암상관적miRNA-Target전록조공망락급기인본체론공능모괴.결과 심편결과사선출6충결직장암간전이조교비전이조표체실조적miRNA(상조적miR-224、miR-1236화miR-622；하조적miR-155、miR-342-5p、miR-363).최현저상조적miR-224불단가이여기대응파기인행사조공작용,이차가이여기타miRNA협동작용우기하유적파기인공능단,행사상응공능.결론 miR-224작위조공망락적핵심,가동시혹이시성조공상관적중요기인공능단,진이완성대결직장암간장전이적전록후수평조공,재간전이과정중기중요작용.
Objective To explore the microRNA expression changes and related characteristics and analyze the corresponding miRNA target genes and their bioinformatics in colorectal cancer with liver metastasis.Methods The fresh specimens of primary CRC were collected in 10 patients during operation,with liver metastasis or without.The miRNA expression levels were compared by miRNA microarray between two groups.Then,target genes were identified using target prediction algorithms.The liver metastasis related miRNA-target networks and gene ontology (GO) bioinformatics analysis were further performed.Results A total of six dysregulated miRNAs were identified in colorectal cancer liver metastasis comparing with no metastasis,including 3 up-regulated miRNAs (miR-224,miR-1236,miR-622) and 3 downregulated miRNAs (miR-155,miR-342-5p,miR-363).miR-224 with most significantly up-regulation played regulation role not only with corresponding target-genes but also downstream genes.Conclusions As a core of the regulation networks,miR-224 can regulate the related gene functional groups simultaneously and asynchronously.It further implements the post-transcriptional regulation and plays a vital role in liver metastasis of colorectal cancer.
