中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2013年
2期
125-128
,共4页
癌,肝细胞%抗原,CD31%多态性,单核苷酸%单倍性
癌,肝細胞%抗原,CD31%多態性,單覈苷痠%單倍性
암,간세포%항원,CD31%다태성,단핵감산%단배성
Carcinoma,hepatocellular%Antigens,CD31%Polymorphism,single nucleotide%Haploidy
目的 研究CD31基因单核苷酸多态性及血清水平与肝癌的相关性.方法 选取190例肝癌患者和210例健康对照者为研究对象,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对CD31基因Leu125Val、Asn563Ser和Gly670Arg单核苷酸多态性进行基因分型,同时采用酶联免疫吸附试验检测血清CD31水平.结果 肝癌组和对照组的CD31基因Asn563Ser和Gly670Arg多态性比较,差异无统计学意义(x2=0.491,P＞0.05),而CD31基因Leu125Val多态性比较差异有统计学意义(x2=10.988,P＜0.05),等位基因频率的相对风险分析发现,Val等位基因携带者患肝癌的风险是Leu等位基因的1.583倍(OR=1.583,95％ CI,1.197 ～2.093,P=0.001)；携带Val等位基因的肝癌患者血清CD31水平显著高于不携带者(x2=10.408,P＜0.05).联合基因型分析显示,CD31基因Leu125Val、Asn563Ser和Gly670Arg单核苷酸多态性存在着强烈的连锁不平衡,与对照组比较,Val-Ser-Arg单倍型携带者显著增加肝癌的发病风险(OR=1.496,95％ CI:1.095 ～2.046,P=0.011). 结论 CD31基因Leu125Va1多态性和Val-Ser-Arg单倍型与肝癌的发病具有相关性,其中Val等位基因可能是肝癌的遗传易感基因,携带Val等位基因的个体可能通过促进CD31的高度表达进而增加肝癌的发病风险.
目的 研究CD31基因單覈苷痠多態性及血清水平與肝癌的相關性.方法 選取190例肝癌患者和210例健康對照者為研究對象,應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)技術對CD31基因Leu125Val、Asn563Ser和Gly670Arg單覈苷痠多態性進行基因分型,同時採用酶聯免疫吸附試驗檢測血清CD31水平.結果 肝癌組和對照組的CD31基因Asn563Ser和Gly670Arg多態性比較,差異無統計學意義(x2=0.491,P＞0.05),而CD31基因Leu125Val多態性比較差異有統計學意義(x2=10.988,P＜0.05),等位基因頻率的相對風險分析髮現,Val等位基因攜帶者患肝癌的風險是Leu等位基因的1.583倍(OR=1.583,95％ CI,1.197 ～2.093,P=0.001)；攜帶Val等位基因的肝癌患者血清CD31水平顯著高于不攜帶者(x2=10.408,P＜0.05).聯閤基因型分析顯示,CD31基因Leu125Val、Asn563Ser和Gly670Arg單覈苷痠多態性存在著彊烈的連鎖不平衡,與對照組比較,Val-Ser-Arg單倍型攜帶者顯著增加肝癌的髮病風險(OR=1.496,95％ CI:1.095 ～2.046,P=0.011). 結論 CD31基因Leu125Va1多態性和Val-Ser-Arg單倍型與肝癌的髮病具有相關性,其中Val等位基因可能是肝癌的遺傳易感基因,攜帶Val等位基因的箇體可能通過促進CD31的高度錶達進而增加肝癌的髮病風險.
목적 연구CD31기인단핵감산다태성급혈청수평여간암적상관성.방법 선취190례간암환자화210례건강대조자위연구대상,응용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술대CD31기인Leu125Val、Asn563Ser화Gly670Arg단핵감산다태성진행기인분형,동시채용매련면역흡부시험검측혈청CD31수평.결과 간암조화대조조적CD31기인Asn563Ser화Gly670Arg다태성비교,차이무통계학의의(x2=0.491,P＞0.05),이CD31기인Leu125Val다태성비교차이유통계학의의(x2=10.988,P＜0.05),등위기인빈솔적상대풍험분석발현,Val등위기인휴대자환간암적풍험시Leu등위기인적1.583배(OR=1.583,95％ CI,1.197 ～2.093,P=0.001)；휴대Val등위기인적간암환자혈청CD31수평현저고우불휴대자(x2=10.408,P＜0.05).연합기인형분석현시,CD31기인Leu125Val、Asn563Ser화Gly670Arg단핵감산다태성존재착강렬적련쇄불평형,여대조조비교,Val-Ser-Arg단배형휴대자현저증가간암적발병풍험(OR=1.496,95％ CI:1.095 ～2.046,P=0.011). 결론 CD31기인Leu125Va1다태성화Val-Ser-Arg단배형여간암적발병구유상관성,기중Val등위기인가능시간암적유전역감기인,휴대Val등위기인적개체가능통과촉진CD31적고도표체진이증가간암적발병풍험.
Objective To investigate the relationship between single nucleotide polymorphisms (SNPs),serum CD31 and HCC.Methods We analyzed three single nucleotide polymorphisms of CD31 gene Leu125Val,Asn563Ser and Gly670Arg in 190 HCC patients and 210 age and sex matched controls in a Chinese population,using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy,and the serum level of CD31 was determined by enzyme-linked immunosorbent assay (ELISA).Results The distributions of CD31 gene Asn563Ser and Gly670Arg polymorphisms were not significantly different between HCC and control group (x2 =0.491,P ＞ 0.05),but the CD31 gene Leu125Val polymorphism was significantly different (x2 =10.988,P ＜ 0.05).The relative risk suffering from HCC of Val allele was 1.583 times of the Leu allele carriers(OR =1.583,95％ CI,1.197-2.093,P =0.001) ; Serum level of CD31 Val allele carriers was significantly higher than that of no carriers(x2 =10.408,P ＜ 0.05).Consistent with the results of the genotyping analyses,CD31 gene Leu125Val,Asn563Ser and Gly670Arg polymorphisms showed strong linkage disequilibrium,the Val-Ser-Arg haplotype was associated with a significantly increased risk of HCC as compared with the controls (OR =1.496,95％CI:1.095-2.046,P =0.011).Conclusions CD31 gene Leu125Val polymorphism and its Val-Ser-Arg haplotype were associated with HCC,Val allele is an important genetic susceptibility gene for HCC.CD31 Val allele carriers may subject to higher risk of HCC with enhanced CD31 expression.
