CAJ | 학술논문

目的探讨姜黄素对体外、体内直肠癌CD133+肿瘤干细胞样细胞群放疗敏感性的影响.方法采用免疫磁珠分选法对直肠癌HRT-18细胞进行干细胞分选后随机分成姜黄素组、放疗组、姜黄素联合放疗组,应用MTT法检测24、48和72 h各组细胞生长抑制情况；采用Annexin V/PI双染流式细胞仪检测各组细胞凋亡情况.将分选的CD133+直肠癌细胞移植于裸鼠右脚垫建立移植瘤模型,将模型鼠分为姜黄素组、放疗组、姜黄素联合放疗组,每组8只,观察瘤体动态生长情况并用原位末端标记技术检测瘤体内细胞凋亡情况.结果低剂量姜黄素联合放疗对直肠癌CD133+肿瘤干细胞样细胞群(24 h生长抑制率:18.7％±1.7％)较放疗组(14.6％±1.0％)具有明显的生长抑制作用(P＜0.01)；姜黄素联合放疗组细胞凋亡率(28.8％±3.7％)与放疗组(13.1％±1.4％)比较差异有统计学意义(P＜0.01)；治疗6d时,姜黄素联合放疗组的瘤体体积为(521 ±79) mm3,与放疗组的(717 ±134) mm3比较差异有统计学意义(P＜0.01)；姜黄素联合放疗组瘤体细胞凋亡指数(26.1％±3.3％)较放疗组(12.0％±2.1％)明显增高,两者比较差异有统计学意义(P＜0.01).结论姜黄素可显著提高CD133+肿瘤干细胞样细胞群的放疗敏感性.
목적 탐토강황소대체외、체내직장암CD133+종류간세포양세포군방료민감성적영향.방법 채용면역자주분선법대직장암HRT-18세포진행간세포분선후수궤분성강황소조、방료조、강황소연합방료조,응용MTT법검측24、48화72 h각조세포생장억제정황；채용Annexin V/PI쌍염류식세포의검측각조세포조망정황.장분선적CD133+직장암세포이식우라서우각점건립이식류모형,장모형서분위강황소조、방료조、강황소연합방료조,매조8지,관찰류체동태생장정황병용원위말단표기기술검측류체내세포조망정황.결과 저제량강황소연합방료대직장암CD133+종류간세포양세포군(24 h생장억제솔:18.7％±1.7％)교방료조(14.6％±1.0％)구유명현적생장억제작용(P＜0.01)；강황소연합방료조세포조망솔(28.8％±3.7％)여방료조(13.1％±1.4％)비교차이유통계학의의(P＜0.01)；치료6d시,강황소연합방료조적류체체적위(521 ±79) mm3,여방료조적(717 ±134) mm3비교차이유통계학의의(P＜0.01)；강황소연합방료조류체세포조망지수(26.1％±3.3％)교방료조(12.0％±2.1％)명현증고,량자비교차이유통계학의의(P＜0.01).결론 강황소가현저제고CD133+종류간세포양세포군적방료민감성.
Objective To investigate the effect of curcumin an extract of a Chinese medical herb on the sensitivity of CD133 + rectal cancer cells to radiotherapy.Methods In vitro experiments:CD133 +cells were purified with immunomagnetic beads from HRT-18 cell line and divided into curcumin group,radiotherapy group and curcumin plus radiotherapy group.MTT assay and Annexin V/PI staining were used to measure the proliferation and apoptosis of the cells.In vivo experiments:Transplanted rectal tumor was established in 46 nude mice and randomly divided into curcumin group,radiotherapy group and curcumin plus radiotherapy group.Tumor size and apoptosis were detected by daily observation and TUNEL staining respectively.Results Curcumin inhibited proliferation and apoptosis of CD133 + rectal cancer cells when combined with radiotherapy.It also significantly increased the growth inhibition of rectal tumor and promoted the apoptosis of rectal cancer in vivo.MTT assay showed that after 24 hours,compared with that of radiotherapy group(14.6％ ± 1.0％),curcumin plus radiotherapy group (18.7％ ± 1.7％) inhibited the growth of the tumor(P ＜ 0.01).Annexin V/PI showed that curcumin plus radiotherapy group (28.8％ ±3.7％) was significantly different from the radiotherapy group(13.1％ ± 1.4％) in cell apoptosis (P ＜0.01).In vivo,after 6 days,tumor volume (521 ± 79) mm3 in curcumin plus radiotherapy group was significantly lower than that of radiotherapy group(717 ± 134) mm3 (P ＜ 0.01) ; TUNEL staining results indicated that the RCST in curcumin plus radiotherapy group (26.1％ ± 3.3％) were higher than that in radiotherapy group (12.0％ ± 2.1％) (P ＜ 0.01).Conclusions Curcumin significantly enhances the radiosensitizing effect for CD133 + rectal cancer cells.