CAJ | 학술논문

目的探讨Caveolin-1对新西兰兔颈总动脉血管吻合口再狭窄的作用,以及与细胞外调节蛋白激酶(extracellular regulated protein kinases,ERK)的关系.方法 40只新西兰兔,随机分为正常对照组、手术组、空转染组和Caveolin-1转染组.行左侧颈总动脉血管端端吻合,并局部转染Caveolin-1质粒(转染组)或空质粒(空转染组).于术后第7天各组中分别取5只新西兰兔血管标本,用于Western blot和逆转录-聚合酶链反应(RT-PCR)检测蛋白和mRNA的表达；其余新西兰兔于术后28 d处死取颈总动脉,通过HE染色观察内膜增生情况,用Image-Pro Plus6.0软件测量内膜与中膜面积比值(IA/MA).结果血管HE染色后测量内膜与中膜面积比值:Caveolin-1转染组的血管内膜/中膜面积比值较手术组降低约50％；与手术组比较,转染组Caveolin-1的mRNA和蛋白的表达明显升高,差异有统计学意义(t=36.59,P＜0.01)；而在高表达Caveolin-1的血管吻合口上,ERK1/2的mRNA的表达以及蛋白质的活性都显著低于手术组(t值分别为:32.64和7.28,两者均P＜0.01).结论 Caveolin-1抑制吻合口再狭窄的作用可能与调节ERK1/2的活化有关.
목적 탐토Caveolin-1대신서란토경총동맥혈관문합구재협착적작용,이급여세포외조절단백격매(extracellular regulated protein kinases,ERK)적관계.방법 40지신서란토,수궤분위정상대조조、수술조、공전염조화Caveolin-1전염조.행좌측경총동맥혈관단단문합,병국부전염Caveolin-1질립(전염조)혹공질립(공전염조).우술후제7천각조중분별취5지신서란토혈관표본,용우Western blot화역전록-취합매련반응(RT-PCR)검측단백화mRNA적표체；기여신서란토우술후28 d처사취경총동맥,통과HE염색관찰내막증생정황,용Image-Pro Plus6.0연건측량내막여중막면적비치(IA/MA).결과 혈관HE염색후측량내막여중막면적비치:Caveolin-1전염조적혈관내막/중막면적비치교수술조강저약50％；여수술조비교,전염조Caveolin-1적mRNA화단백적표체명현승고,차이유통계학의의(t=36.59,P＜0.01)；이재고표체Caveolin-1적혈관문합구상,ERK1/2적mRNA적표체이급단백질적활성도현저저우수술조(t치분별위:32.64화7.28,량자균P＜0.01).결론 Caveolin-1억제문합구재협착적작용가능여조절ERK1/2적활화유관.
Objective To investigate the effect of Caveolin-1 on extracellular regulated protein kinases of rabbit carotid artery anastomotic restenosis.Methods 40 New Zealand white rabbits were randomly divided into normal control group,carotid artery end to end anastomosis surgical group,empty vector transfection on the site of anastomosis group and Caveolin-1 transfected group.Left carotid artery endto-end anastomosis was performed,and the mixture of Caveolin-1 plasmid and liposome lipofectin 2000 (transfected group) or empty plasmid and lipofectin 2000 mixture (empty vector group) were transfected on anastomosis.Specimens were taken at 7 d after surgery for Western blot and RT-PCR to detect the expression of protein and mRNA.Specimens were taken for HE staining at 28 d to observe the proliferation of intima,and measured the ratio of intima/media area by Image-Pro Plus 6.0 software.Results Compared with surgical group,the ratio of intima/media area in Caveolin-1 transfected group decreased by about 50％.Compared with surgical group and empty vector group,the Caveolin-1 mRNA expression and protein activity significantly increased (t =36.59,P ＜ 0.01) ; the ERK1/2 mRNA expression and protein activity significantly decreased on rabbit carotid artery anastomotic site in Caveolin-1 transfected group (t =32.64and 7.27,P ＜ 0.01).Conclusions Caveolin-1 inhibits anastomotic restenosis possibly by regulating the activation of ERK.