CAJ | 학술논문

目的探讨血浆微小RNA在早期胃癌中的表达情况,研究其在胃癌发生发展中的意义.方法本研究首先对30例胃癌患者和30例健康对照人群血浆中的15种微小RNA(miR-1、miR-106a、miR-106b、miR-17-5p、miR-20a、miR-21、miR-221、miR-27a、miR-34、miR-376c、miR-378、miR-423-5p、miR-451、miR-486、miR-744)进行检测,然后通过定量逆转录聚合酶链反应来确定另外60例胃癌患者和60例健康对照人群的血浆微小RNA是否存在差异.通过受试者工作特征曲线(ROC曲线)下面积来检测被筛查出的血浆微小RNA诊断胃癌的敏感性和特异性.结果胃癌患者的3种血浆微小RNA(miR-106b、miR-20a和miR-221)水平较健康对照人群有显著增高(P＜0.05).用miR-106b、miR-20a和miR-221检测胃癌的ROC曲线下面积分别为0.773(95％CI0.776～0.841)、0.859(95％CI0.805～ 0.914)和0.796(95％ CI0.726 ～0.866).胃癌患者的这3种微小RNA在每一个TNM分期中的表达水平与健康对照人群比较差异有统计学意义(P＜0.05),而在各TNM分期表达水平之间比较差异无统计学意义(P＞0.05).结论血浆miR-106b、miR-20a和miR-221可作为胃癌早期检测潜在的生物标志物.
목적 탐토혈장미소RNA재조기위암중적표체정황,연구기재위암발생발전중적의의.방법 본연구수선대30례위암환자화30례건강대조인군혈장중적15충미소RNA(miR-1、miR-106a、miR-106b、miR-17-5p、miR-20a、miR-21、miR-221、miR-27a、miR-34、miR-376c、miR-378、miR-423-5p、miR-451、miR-486、miR-744)진행검측,연후통과정량역전록취합매련반응래학정령외60례위암환자화60례건강대조인군적혈장미소RNA시부존재차이.통과수시자공작특정곡선(ROC곡선)하면적래검측피사사출적혈장미소RNA진단위암적민감성화특이성.결과 위암환자적3충혈장미소RNA(miR-106b、miR-20a화miR-221)수평교건강대조인군유현저증고(P＜0.05).용miR-106b、miR-20a화miR-221검측위암적ROC곡선하면적분별위0.773(95％CI0.776～0.841)、0.859(95％CI0.805～ 0.914)화0.796(95％ CI0.726 ～0.866).위암환자적저3충미소RNA재매일개TNM분기중적표체수평여건강대조인군비교차이유통계학의의(P＜0.05),이재각TNM분기표체수평지간비교차이무통계학의의(P＞0.05).결론 혈장miR-106b、miR-20a화miR-221가작위위암조기검측잠재적생물표지물.
Objective To study the exprssion of miR-106b,miR-20a and miR-221 in early development of gastric cancer.Methods The plasma expression of 15 selected microRNAs (miR-1,miR-106a,miR-106b,miR-17-5p,miR-20a,miR-21,miR-221,miR-27a,miR-34,miR-376c,miR-378,miR-423-5p,miR-451,miR-486,miR-744) in 30 gastric cancer (GC) patients and 30 age-and gendermatched healthy controls were measured and then the differences of those micro-RNAs expressions in other 60 GC patients and 60 matched controls were evaluated by using quantitative reverse transcription polymerase chain reaction.The areas under the receiver operating characteristic (ROC) curves were used to test the sensitivity and specificity of GC diagnosis using these identified plasma microRNAs.Results Three plasma microRNAs miR-106b,miR-20a,and miR-221,were significantly elevated in GC patients than in healthy controls (P ＜ 0.05).Furthermore,the areas under the ROC curves using miR-106b,miR-20a,and miR-221 for GC diagnosis were 0.773 (95％ CI 0.776-0.841),0.859 (95％ CI 0.805-0.914),and 0.796 (95％ CI 0.726-0.866),respectively.These three microRNAs were on a statistically significant elevation in GC patients compared with healthy controls at each of the four stages.However,there were no significant differences in the plasma levels of the three microRNAs among the four TNM stages (P ＞ 0.05).Conclusions Plasma miR-106b,miR-20a,and miR-221 may be used as biomarkers for the detection of early GC.