CAJ | 학술논문

目的探讨cortactin在结直肠癌组织、癌前病变(腺瘤性息肉)、癌旁正常组织中的表达及其意义,结直肠癌细胞cortactin表达水平与细胞分裂状态的关系.方法采用免疫组织化学方法,检测cortactin在结直肠癌组织、癌前病变(腺瘤性息肉)、癌旁正常组织中的表达.用Kaplan-Meier法比较cortactin不表达或者低表达患者组与高表达患者组5年生存率.分析cortactin在结直肠癌组织中的表达与临床病理参数的关系.FISH技术观察cortactin编码基因CTTN在结直肠组织中的扩增.体外合成cortactin siRNA后转染结直肠癌细胞株SW620,用Western blot和免疫荧光观察转染后cortactin表达量的变化及细胞分裂状态.结果 Cortactin在结直肠癌组织中的表达明显高于腺瘤性息肉和癌旁结直肠正常组织.在结直肠癌组织中cortactin表达增高与结直肠癌不良分化(P＜0.01)、有淋巴结转移(P =0.006)、TNM分期(P =0.022)相关.Cortactin阴性表达或者低表达组结直肠癌患者术后5年生存率明显高于cortactin高表达组.结直肠癌组织中cortactin编码基因CTTN扩增明显,结肠腺瘤与癌旁正常组织中未见扩增.导入cortactin siRNA的结直肠癌细胞cortaetin蛋白表达下降.结论 Cortactin异常表达可能与结直肠癌的生长、侵袭及转移有关.
목적 탐토cortactin재결직장암조직、암전병변(선류성식육)、암방정상조직중적표체급기의의,결직장암세포cortactin표체수평여세포분렬상태적관계.방법 채용면역조직화학방법,검측cortactin재결직장암조직、암전병변(선류성식육)、암방정상조직중적표체.용Kaplan-Meier법비교cortactin불표체혹자저표체환자조여고표체환자조5년생존솔.분석cortactin재결직장암조직중적표체여림상병리삼수적관계.FISH기술관찰cortactin편마기인CTTN재결직장조직중적확증.체외합성cortactin siRNA후전염결직장암세포주SW620,용Western blot화면역형광관찰전염후cortactin표체량적변화급세포분렬상태.결과 Cortactin재결직장암조직중적표체명현고우선류성식육화암방결직장정상조직.재결직장암조직중cortactin표체증고여결직장암불량분화(P＜0.01)、유림파결전이(P =0.006)、TNM분기(P =0.022)상관.Cortactin음성표체혹자저표체조결직장암환자술후5년생존솔명현고우cortactin고표체조.결직장암조직중cortactin편마기인CTTN확증명현,결장선류여암방정상조직중미견확증.도입cortactin siRNA적결직장암세포cortaetin단백표체하강.결론 Cortactin이상표체가능여결직장암적생장、침습급전이유관.
Objective To investigate cortactin expression in malignant colorectal tissues and corresponding adjacent non-cancerous colon tissues,precancerous lesions (adenomatous polyps) and the relationship between the expression of cortactin and cell division in colorectal cancer cells.Methods The expression of cortactin was detected by immunohistochemistry in colorectal cancer,colorectal adenomatous polyp (precancerous lesions) and colorectal tissues adjacent to adenocarcinomas (normal tissues).Kaplan-Meier method was employed to compare the survival between the groups.Cortactin expression and cell division were detected by Western blot and immunofluorescence in SW-620 colon cancer cells treated with cortactin siRNA.Results The positive expression rate of cortactin was significantly higher in colorectal cancer tissues than in adenomatous polyp tissues and pericarcinomatous normal tissues.Overexpression of cortactin in colorectal cancer tissues was correlated with poor differentiation (P ＜ 0.01),lymph node metastasis (P =0.006),and TNM stage (P =0.022).The 5 year survival rate of the group of negative/weak positive expression of eortactin was significantly higher than the group of strong positive expression of cortactin.CTTN gene amplification in colorectal cancer tissues was obvious.Cortactin siRNA induction caused a lower cortactin protein expression in colorectal cancer cells.Conclusions It is suggested that the excessive expression of cortactin contributes to the growth of cancer cells in colorectal cancer.