中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2013年
8期
531-535
,共5页
王玉飞%石聪聪%焦连龙%郭笑逸%张帅%付一群%贺颖
王玉飛%石聰聰%焦連龍%郭笑逸%張帥%付一群%賀穎
왕옥비%석총총%초련룡%곽소일%장수%부일군%하영
脑缺血%卒中%5-脂氧合酶激活蛋白质类%多态现象,遗传%基因环境相互作用
腦缺血%卒中%5-脂氧閤酶激活蛋白質類%多態現象,遺傳%基因環境相互作用
뇌결혈%졸중%5-지양합매격활단백질류%다태현상,유전%기인배경상호작용
Brain ischemia%Stroke%5-Lipoxygenase-activating proteins%Polymorphism,genetic%Gene-environment interaction
目的 探讨5-脂氧合酶激活蛋白基因(ALOX5AP基因)启动子区rs17222919位点基因多态性与环境因素在河南汉族人群缺血性卒中(IS)发病中的交互作用.方法 对河南省汉族622例IS患者和631名健康对照者进行环境危险因素调查,运用TaqMan-PCR技术对ALOX5AP基因rs17222919位点进行基因分型,探讨其与IS发生的相关性,并根据交互作用系数(Υ,Υ=βeg/βe)判断交互作用及其作用模型.结果 IS组rs17222919位点的TG(189/622,30.4％)、GG(18/622,2.9％)基因型频率及G等位基因频率(225/1244,18.1％)显著低于健康对照组(221/631,35.0％；31/631,4.9％;283/1262,22.4％),差异具有统计学意义(x2 =4.117,P=0.042;x2=4.457,P=0.035;x2 =7.294,P=0.007).TG+GG基因型与高血压、糖尿病、吸烟之间在IS发病中均存在负向交互作用(y =0.943、0.922、0.830),其作用模型均为超相乘模型.结论 IS的发生是遗传及环境因素相互作用的结果.在河南汉族人群中,rs17222919位点G等位基因可能对IS发病的环境危险因素高血压、糖尿病、吸烟暴露的效应具有减弱作用.
目的 探討5-脂氧閤酶激活蛋白基因(ALOX5AP基因)啟動子區rs17222919位點基因多態性與環境因素在河南漢族人群缺血性卒中(IS)髮病中的交互作用.方法 對河南省漢族622例IS患者和631名健康對照者進行環境危險因素調查,運用TaqMan-PCR技術對ALOX5AP基因rs17222919位點進行基因分型,探討其與IS髮生的相關性,併根據交互作用繫數(Υ,Υ=βeg/βe)判斷交互作用及其作用模型.結果 IS組rs17222919位點的TG(189/622,30.4％)、GG(18/622,2.9％)基因型頻率及G等位基因頻率(225/1244,18.1％)顯著低于健康對照組(221/631,35.0％；31/631,4.9％;283/1262,22.4％),差異具有統計學意義(x2 =4.117,P=0.042;x2=4.457,P=0.035;x2 =7.294,P=0.007).TG+GG基因型與高血壓、糖尿病、吸煙之間在IS髮病中均存在負嚮交互作用(y =0.943、0.922、0.830),其作用模型均為超相乘模型.結論 IS的髮生是遺傳及環境因素相互作用的結果.在河南漢族人群中,rs17222919位點G等位基因可能對IS髮病的環境危險因素高血壓、糖尿病、吸煙暴露的效應具有減弱作用.
목적 탐토5-지양합매격활단백기인(ALOX5AP기인)계동자구rs17222919위점기인다태성여배경인소재하남한족인군결혈성졸중(IS)발병중적교호작용.방법 대하남성한족622례IS환자화631명건강대조자진행배경위험인소조사,운용TaqMan-PCR기술대ALOX5AP기인rs17222919위점진행기인분형,탐토기여IS발생적상관성,병근거교호작용계수(Υ,Υ=βeg/βe)판단교호작용급기작용모형.결과 IS조rs17222919위점적TG(189/622,30.4％)、GG(18/622,2.9％)기인형빈솔급G등위기인빈솔(225/1244,18.1％)현저저우건강대조조(221/631,35.0％；31/631,4.9％;283/1262,22.4％),차이구유통계학의의(x2 =4.117,P=0.042;x2=4.457,P=0.035;x2 =7.294,P=0.007).TG+GG기인형여고혈압、당뇨병、흡연지간재IS발병중균존재부향교호작용(y =0.943、0.922、0.830),기작용모형균위초상승모형.결론 IS적발생시유전급배경인소상호작용적결과.재하남한족인군중,rs17222919위점G등위기인가능대IS발병적배경위험인소고혈압、당뇨병、흡연폭로적효응구유감약작용.
Objective To explore the interaction between polymorphisms of rs17222919 which located in the 5-1ipoxygenase-activating protein(ALOX5AP) gene promoter and environmental risk factors in ischemic stroke(IS).Methods We conducted a case-control study involving a total of 622 cases and 631 unrelated healthy controls which were selected from Henan Han populations,and the environment risk factors were recorded.Genotyping aimed at detecting both genetic and environmental factors in relation to IS was performed by TaqMan-polymerase chain reaction technology while interaction indexes (Υ) were calculated to determine interactions and their role models.Results The rs17222919 TG (189/622,30.4％),GG (18/622,2.9％)genotype frequencies and G (225/1244,18.1％)allele frequencies in IS subjects were significantly lower than those in controls (221/631,35.0％ ; 31/631,4.9％ ; 283/1262,22.4％ ; x2 =4.117,P =0.042 ; x2 =4.457,P =0.035 ; x2 =7.294,P =0.007).Negative interactions between TG + GG genotype and hypertension,diabetes or cigarette smoking in the occurrence of IS (Υ =0.943,0.922,0.830) were observed,whose role models were all super-multiplicative models.Conclusions According to our study,ischemic stroke is the result of the interaction of genetic and environmental factors and G allele of rs17222919 may have weakened the role of environmental factors for hypertension,diabetes and cigarette smoking in IS incidence.
