CAJ | 학술논문

目的探讨ENC1基因与无功能垂体腺瘤(NFPAs)的发生及侵袭性的相关性,并比较其在裸细胞瘤和瘤样细胞瘤这两种NFPAs亚型中表达是否存在差异.方法选择23例裸细胞瘤、20例瘤样细胞瘤和3例正常垂体组织.采用QPCR和Western blot检测ENC1在正常垂体组织和肿瘤组织中的mRNA和蛋白表达情况;采用免疫组化检测ENC1蛋白在肿瘤中的表达情况.结果 QPCR和Western blot结果显示在裸细胞瘤和瘤样细胞瘤中ENC1的表达明显低于正常垂体组织,且ENC1在侵袭性裸细胞瘤的表达明显低于非侵袭性裸细胞瘤的表达(P＜0.0001和P＜0.005),而ENC1在侵袭性和非侵袭性瘤样细胞瘤中的表达差异无统计学意义(P＞0.05).免疫组化结果显示ENC1蛋白在正常垂体组织表达呈强阳性,在非侵袭性裸细胞瘤为中等表达,而在侵袭性裸细胞瘤中呈弱阳性.结论 ENC1与裸细胞瘤的侵袭性相关,而与瘤样细胞瘤的侵袭性不相关,其可能是与裸细胞瘤侵袭性特异相关的一个重要基因.
목적 탐토ENC1기인여무공능수체선류(NFPAs)적발생급침습성적상관성,병비교기재라세포류화류양세포류저량충NFPAs아형중표체시부존재차이.방법 선택23례라세포류、20례류양세포류화3례정상수체조직.채용QPCR화Western blot검측ENC1재정상수체조직화종류조직중적mRNA화단백표체정황;채용면역조화검측ENC1단백재종류중적표체정황.결과 QPCR화Western blot결과현시재라세포류화류양세포류중ENC1적표체명현저우정상수체조직,차ENC1재침습성라세포류적표체명현저우비침습성라세포류적표체(P＜0.0001화P＜0.005),이ENC1재침습성화비침습성류양세포류중적표체차이무통계학의의(P＞0.05).면역조화결과현시ENC1단백재정상수체조직표체정강양성,재비침습성라세포류위중등표체,이재침습성라세포류중정약양성.결론 ENC1여라세포류적침습성상관,이여류양세포류적침습성불상관,기가능시여라세포류침습성특이상관적일개중요기인.
Objective Non-functioning pituitary adenomas (NFPAs) may be locally invasive.Surgery is a treatment option,but unlike the case for functional pituitary adenomas,there are almost no drug treatments available for NFPAs.To identify the relationship of ENC1 gene with invasiveness in NFPAs and to observe whether the ENC1 expression level was different between invasive null cell adenomas and invasive oncocytomas.Methods In this study,we selected 23 null cell adenomas (14 invasive and 9 non-invasive tumors),20 oncocytomas (10 invasive and 10 non-invasive tumors) and 3 normal pituitary glands.ENC1 mRNA and protein expression level in all pituitary tumors and normal pituitary glands was detected by QPCR and western-blot.ENC1 protein expression level was observed by immunohistochemistry (IHC).Results Low levels of ENC1 were found in null cell adenomas and oncocytoma,while high levels were detected in normal pituitary glands.ENC1 mRNA expression level was reduced in invasive null cell adenomas compared to non-invasive null cell adenomas (P ＜ 0.0001) but the results were not found between invasive and non-invasive oncocytomas.Western blot analyses showed that ENC1 expression was significantly lower in invasive null cell adenomas compared with non-invasive null cell adenomas (P ＜ 0.005) and normal pituitary glands.The immunostaining results indicated the ENC1 staining was lighter in invasive null cell adenomas than in non-invasive null cell adenomas,and it was darker in normal pituitary tissues.Conclusion ENC1 may play an important role in invasive null cell adenomas.It could be a specific biomarker for invasive null cell adenomas.