CAJ | 학술논문

目的明确颅内动静脉畸形(AVM)病灶内信号转导与转录活化因子3(STAT3)及其磷酸化形式的表达.方法 35例颅内AVM患者行开颅脑AVM切除术,留取手术切除标本,5例脑外伤行内减压患者脑组织为正常对照.应用免疫组化方法检测STAT3及其磷酸化形式P-STAT3(Tyr705)、P-STAT3(Ser727)的表达,并比较出血和未出血、术前栓塞和未栓塞以及Sptzer-Martin分级低级别和高级别患者STAT3及其磷酸化形式表达是否存在差异.结果对照组中仅2例有STAT3弱表达,35例AVM患者STAT3、P-STAT3(Tyr705)和P-STAT3(Ser727)表达阳性率分别为100％、74％(26/35)和77％(27/35).P-STAT3(Tyr705)在栓塞组表达高于未栓塞组(P＜0.05).结论 STAT3及其磷酸化形式在颅内AVM中表达增高,STAT3通路激活可能参与颅内AVM的病理发展过程;栓塞可能促使颅内AVM病灶内STAT3活化.
목적 명학로내동정맥기형(AVM)병조내신호전도여전록활화인자3(STAT3)급기린산화형식적표체.방법 35례로내AVM환자행개로뇌AVM절제술,류취수술절제표본,5례뇌외상행내감압환자뇌조직위정상대조.응용면역조화방법검측STAT3급기린산화형식P-STAT3(Tyr705)、P-STAT3(Ser727)적표체,병비교출혈화미출혈、술전전새화미전새이급Sptzer-Martin분급저급별화고급별환자STAT3급기린산화형식표체시부존재차이.결과 대조조중부2례유STAT3약표체,35례AVM환자STAT3、P-STAT3(Tyr705)화P-STAT3(Ser727)표체양성솔분별위100％、74％(26/35)화77％(27/35).P-STAT3(Tyr705)재전새조표체고우미전새조(P＜0.05).결론 STAT3급기린산화형식재로내AVM중표체증고,STAT3통로격활가능삼여로내AVM적병리발전과정;전새가능촉사로내AVM병조내STAT3활화.
Objective To investigate the expression of STAT3 (signal transducers and activators of transcription-3) and phosphorylated STAT3 in patients of cerebral arteriovenous malformation (AVM).Methods 35 patients who underwent cerebral AVM removal had been enrolled in this study.Five patients who underwent decompression for brain trauma were normal control.The resected AVM nidus and control tissue were under immunohistochemisty analysis for STAT3,P-STAT3(Tyr705) and P-STAT3(Ser727).Results The expression of STAT3,P-STAT3 (Tyr705) and P-STAT3 (Ser727) were 100％ (35/35),74％ (24/35) and 77％ (24/35) respectively in AVM group.except weakly positive express of STAT3 in two patient,P-STAT3 (Tyr705) and P-STAT3 (Ser727) were all negative in control group.the expression of P-STAT3 (Tyr705) showed more intensive level in thrombosis group than unthrombosis group (P ＜ 0.05).Conclusions STAT3 was overexpressed and activated in cerebral AVM.The activation of STAT3 signal transduction way may mediate the blood vessels remolding and neovasculization in cerebral AVM.Thrombosis maybe increase STAT3 phosphorylation.