CAJ | 학술논문

目的探讨缝隙连接在蛛网膜下腔出血后脑血管痉挛病理机制中的作用.方法 120只SD大鼠随机分对照组、假手术组、手术组及治疗组(n=30),且各组分第1、3、5、7、14天共5亚组(n=6) 视交叉前池二次注血法建立SD大鼠蛛网膜下腔出血动物模型,HE染色鉴定模型,压力机动仪测量基底动脉外径,Western blot检测基底动脉缝隙连接蛋白.结果手术组较对照组及假手术组变化显著(P＜0.01),手术组基底动脉外径、Cx 40减低,Cx 43、Cx 45升高,手术组组内不同时间变化显著(P＜0.01),基底动脉第1天开始痉挛,第7天达峰值,第14 d逐渐缓解;治疗组较手术组变化显著(P＜0.01),治疗组基底动脉外径、Cx40升高,Cx43、Cx45减低,治疗组组内无显著变化(P＞0.05),18β-甘草次酸明显抑制脑血管痉挛;对照组、假手术组及治疗组间无显著变化(P ＞0.05);Cx37未检测到;Cx40与基底动脉呈正相关(P＜0.01,r=0.823),Cx43与 Cx45呈正相关(P＜0.01,r=0.939),Cx40与 Cx43、Cx45呈负相关(P＜0.01,r=0.840;P＜0.01,r=-0.839),基底动脉直径与Cx43、Cx45呈负相关(P＜0.01,r=0.828;P＜0.01,r=-0.823).结论 Cx43及Cx45在蛛网膜下腔出血后脑血管痉挛的发生中可能起促进作用,而Cx40则相反;蛛网膜下腔出血后,Cx43与Cx45间存在正相关,Cx40分别与 Cx43及Cx45 间存在负相关;18β-甘草次酸可缓解蛛网膜下腔出血后脑血管痉挛.
목적 탐토봉극련접재주망막하강출혈후뇌혈관경련병리궤제중적작용.방법 120지SD대서수궤분대조조、가수술조、수술조급치료조(n=30),차각조분제1、3、5、7、14천공5아조(n=6) 시교차전지이차주혈법건립SD대서주망막하강출혈동물모형,HE염색감정모형,압력궤동의측량기저동맥외경,Western blot검측기저동맥봉극련접단백.결과 수술조교대조조급가수술조변화현저(P＜0.01),수술조기저동맥외경、Cx 40감저,Cx 43、Cx 45승고,수술조조내불동시간변화현저(P＜0.01),기저동맥제1천개시경련,제7천체봉치,제14 d축점완해;치료조교수술조변화현저(P＜0.01),치료조기저동맥외경、Cx40승고,Cx43、Cx45감저,치료조조내무현저변화(P＞0.05),18β-감초차산명현억제뇌혈관경련;대조조、가수술조급치료조간무현저변화(P ＞0.05);Cx37미검측도;Cx40여기저동맥정정상관(P＜0.01,r=0.823),Cx43여 Cx45정정상관(P＜0.01,r=0.939),Cx40여 Cx43、Cx45정부상관(P＜0.01,r=0.840;P＜0.01,r=-0.839),기저동맥직경여Cx43、Cx45정부상관(P＜0.01,r=0.828;P＜0.01,r=-0.823).결론 Cx43급Cx45재주망막하강출혈후뇌혈관경련적발생중 가능기촉진작용,이Cx40칙상반;주망막하강출혈후,Cx43여Cx45간존재정상관,Cx40분별여 Cx43급Cx45 간존재부상관;18β-감초차산가완해주망막하강출혈후뇌혈관경련.
Objective To investigate the role of gap junction in the pathomechanism and treatment of cerebral vasospasm after experimental subarachnoid hemorrhage.Methods HE dyed was used to identify the model.The diameters of basilar arteries were determined by pressure myograph.The connexins in the basilar arteries of different groups were examined by western blot.Results The diameters,connexins in basilar arteries were compared in and between groups respectively.There are more significant differences in operation group compared with control,sham and treatment group(P ＜ 0.01),that is Cx40 and basilar arteries diameters are reduced,Cx43 and Cx45 are elevated.But the differences in and between Control,sham and treatment group are not obvious (P ＞ 0.05); Cx37 can't examined; Cx40 and basilar arteries diameters are positive correlation(P ＜0.01,r =0.823),with Cx43 and Cx45 are negative correlation(P ＜0.01,r =-0.840; P ＜0.01,r =-0.839).Cx43 and Cx45 with basilar arteries diameters are negative(P ＜0.01,r =-0.828;P ＜0.01,r =-0.823).Cx43 and Cx45 are positive correlation(P ＜ 0.01,r =0.939).Conclusions Cx43 and Cx45 proteins play an important role which is promotion in the pathomechanism of cerebral vasospasm after experimental subarachnoid hemorrhage,but Cx40 is opposite; There are correlation among Cx40 and Cx43 Cx45,Cx43 and Cx45 are positively correlated,Cx40 and Cx43,Cx45 are negatively correlated,respectively.Gap junction inhibitor 18β-glycyrrhetinic-acid can relieve cerebral vasospasm after experimental subarachnoid hemorrhage in rats in vivo.