CAJ | 학술논문

目的明确Wnt/PCP-JNK通路在牛磺酸预防神经畸形中的介导作用及机制.方法采用维甲酸诱导昆明小鼠胚胎神经管畸形模型及设立应用牛磺酸预防的动物模型,采用免疫组化、蛋白质印迹法(Western blot)检测Wnt/PCP-JNK通路中Dvl、RhoA、p-JNK/JNK在胚胎发育神经管中的表达变化,及各个蛋白表达变化的相互关系.结果健康对照组中未见神经管畸形胚胎;采用免疫组化、Western Blot检测Dvl、RhoA、p-JNK/JNK表达较低.维甲酸致畸组神经畸形发生率为57.1％,较健康对照组明显增高(P＜0.05);Dvl、RhoA、p-JNK/JNK表达较健康对照组明显增高(P＜0.05).牛磺酸干预组神经畸形发生率为31.7％,较维甲酸致畸组明显减低(P＜0.05);Dvl、RhoA、p-JNK/JNK表达较维甲酸致畸组明显降低(P＜0.05).结论 Wnt/PCP-JNK通路介导了牛磺酸预防神经管畸形发生的过程
목적 명학Wnt/PCP-JNK통로재우광산예방신경기형중적개도작용급궤제.방법 채용유갑산유도곤명소서배태신경관기형모형급설립응용우광산예방적동물모형,채용면역조화、단백질인적법(Western blot)검측Wnt/PCP-JNK통로중Dvl、RhoA、p-JNK/JNK재배태발육신경관중적표체변화,급각개단백표체변화적상호관계.결과 건강대조조중미견신경관기형배태;채용면역조화、Western Blot검측Dvl、RhoA、p-JNK/JNK표체교저.유갑산치기조신경기형발생솔위57.1％,교건강대조조명현증고(P＜0.05);Dvl、RhoA、p-JNK/JNK표체교건강대조조명현증고(P＜0.05).우광산간예조신경기형발생솔위31.7％,교유갑산치기조명현감저(P＜0.05);Dvl、RhoA、p-JNK/JNK표체교유갑산치기조명현강저(P＜0.05).결론 Wnt/PCP-JNK통로개도료우광산예방신경관기형발생적과정
Objective To clarify the mediating role and mechanism of Wnt/PCP-JNK pathway in prevention of neural malformation of taurine.Methods Kunming mouse model of embryonic neural tube defects were induced by using retinoic acid and the animal models of these defects were established by using taurine.Immunohistochemistry,Western Blotting were used to detect Dvl,RhoA,p-JNK/JNK expression of Wnt/PCP-JNK pathway in the neural tube during embryonic development and the relationships among expression changes of various proteins.Results In the control group,no embryos with neural tube defects were observed and low expressions of Dvl,RhoA and p-JNK/JNK were detected by using immunohistochemistry and Western blotting.In the teratogenic group,the incidence of neurological malformation (57.1％) was significantly higher than that in the control group (P ＜ 0.05).Compared with the control group,the expressions of Dvl,RhoA and p-JNK/JNK significantly increased (P ＜ 0.05).The incidence of neurological malformation following taurine treatment was 31.7％,significant lower than that in the teratogenic group (P ＜0.05).The expressions of Dvl,RhoA and p-JNK/ JNK following taurine intervention significantly decreased compared to the teratogenic group (P ＜ 0.05).Conclusion Wnt/PCP-JNK pathway mediates the process for the taurine to prevent the occurrence of neural malformation.